Proteomic analysis of the excretory/secretory products and antigenic proteins of Echinococcus granulosus adult worms from infected dogs

Wang, Ying; Xiao, Di; Shen, Yujuan; Han, Xiuming; Zhao, Fei; Li, Xiaohong; Wu, Wei; Zhou, He-Jun; Zhang, Jianzhong; Cao, Jianping

doi:10.1186/s12917-015-0423-8

Cited by 27 publications

(14 citation statements)

References 39 publications

(30 reference statements)

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“…Our results show that among cysticercoid proteins of H . diminuta , there are also proteins previously noticed as antigens or proteins being involved in mechanisms of host immune evasion and/or immune modulation in other helminths such as: actin, calpain, HSP70 and HSP60, major egg antigen, myosin, paramyosin [ 14 , 19 , 21 , 28 , 29 ]; however, we also pointed out some molecules with roles in parasite-host crosstalk that have never been considered in cestodes (e.g. procollagen, collagens, RBBP).…”

Section: Discussionmentioning

confidence: 99%

“…Better understanding of a parasite’s adaptations to its way of life and the complexity of interactions between the parasite and the host is possible by introduction of modern techniques of proteomic and genomic research [ 10 – 13 ]. Proteomic studies of helminths have shown new aspects in the parasite-host interrelations involving selected species of tapeworms Echinococcus granulosus [ 14 – 19 ] , Echinococcus multilocularis [ 20 , 21 ] and Taenia solium [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta

et al. 2017

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BackgroundA wide range of molecules are used by tapeworm metacestodes to establish successful infection in the hostile environment of the host. Reports indicating the proteins in the cestode-host interactions are limited predominantly to taeniids, with no previous data available for non-taeniid species. A non-taeniid, Hymenolepis diminuta, represents one of the most important model species in cestode biology and exhibits an exceptional developmental plasticity in its life-cycle, which involves two phylogenetically distant hosts, arthropod and vertebrate.ResultsWe identified H. diminuta cysticercoid proteins that were recognized by sera of H. diminuta-infected rats using two-dimensional gel electrophoresis (2DE), 2D-immunoblotting, and LC-MS/MS mass spectrometry. Proteomic analysis of 42 antigenic spots revealed 70 proteins. The largest number belonged to structural proteins and to the heat-shock protein (HSP) family. These results show a number of the antigenic proteins of the cysticercoid stage, which were present already in the insect host prior to contact with the mammal host. These are the first parasite antigens that the mammal host encounters after the infection, therefore they may represent some of the molecules important in host-parasite interactions at the early stage of infection.ConclusionsThese results could help in understanding how H. diminuta and other cestodes adapt to their diverse and complex parasitic life-cycles and show universal molecules used among diverse groups of cestodes to escape the host response to infection.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2519-4) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta

et al. 2017

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“…and the host that may help differentiate subpopulations of patients. Characterization of ES proteins from E. granulosus adult worms (119) and protoscoleces (120,121) and E. multilocularis protoscoleces (122) also shows promise for identification of potential diagnostic markers.…”

Section: Figmentioning

confidence: 99%

Echinococcosis: Advances in the 21st Century

Wen¹,

Vuitton²,

et al. 2019

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SUMMARY Echinococcosis is a zoonosis caused by cestodes of the genus Echinococcus (family Taeniidae). This serious and near-cosmopolitan disease continues to be a significant public health issue, with western China being the area of highest endemicity for both the cystic (CE) and alveolar (AE) forms of echinococcosis. Considerable advances have been made in the 21st century on the genetics, genomics, and molecular epidemiology of the causative parasites, on diagnostic tools, and on treatment techniques and control strategies, including the development and deployment of vaccines. In terms of surgery, new procedures have superseded traditional techniques, and total cystectomy in CE, ex vivo resection with autotransplantation in AE, and percutaneous and perendoscopic procedures in both diseases have improved treatment efficacy and the quality of life of patients. In this review, we summarize recent progress on the biology, epidemiology, diagnosis, management, control, and prevention of CE and AE. Currently there is no alternative drug to albendazole to treat echinococcosis, and new compounds are required urgently. Recently acquired genomic and proteomic information can provide a platform for improving diagnosis and for finding new drug and vaccine targets, with direct impact in the future on the control of echinococcosis, which continues to be a global challenge.

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“…The EgEnolase in various parasites shows antigenic properties and in E. granulosus is also involved in the invasion and evasion mechanisms through the immunomodulation of the host immune system. 77,78 An epitope-based vaccine administrated to animal or human host body should ideally (i) be immunogenic sufficiently, (ii) has a high ability to stimulate significant humoral and cellular immune response, and finally (iii) activate the memory cells against the pathogenic epitopes. In our study, seven E. granulosus enolase protein sequences were checked in terms of antigenicity and the best ones were selected for further immunoinformatics analysis (Table S1, Supplementary file 1).…”

Section: Discussionmentioning

confidence: 99%

A multi-method and structure-based in silico vaccine designing against Echinococcus granulosus through investigating enolase protein

et al. 2019

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Introduction: Hydatid disease is a ubiquitous parasitic zoonotic disease, which causes different medical, economic and serious public health problems in some parts of the world. The causal organism is a multi-stage parasite named Echinococcus granulosus whose life cycle is dependent on two types of mammalian hosts viz definitive and intermediate hosts. Methods: In this study, enolase, as a key functional enzyme in the metabolism of E. granulosus (EgEnolase), was targeted through a comprehensive in silico modeling analysis and designing a host-specific multi-epitope vaccine. Three-dimensional (3D) structure of enolase was modeled using MODELLER v9.18 software. The B-cell epitopes (BEs) were predicted based on the multi-method approach and via some authentic online predictors. ClusPro v2.0 server was used for docking-based T-helper epitope prediction. The 3D structure of the vaccine was modeled using the RaptorX server. The designed vaccine was evaluated for its immunogenicity, physicochemical properties, and allergenicity. The codon optimization of the vaccine sequence was performed based on the codon usage table of E. coli K12. Finally, the energy minimization and molecular docking were implemented for simulating the vaccine binding affinity to the TLR-2 and TLR-4 and the complex stability. Results: The designed multi-epitope vaccine was found to induce anti-EgEnolase immunity which may have the potential to prevent the survival and proliferation of E. granulosus into the definitive host. Conclusion: Based on the results, this step-by-step immunoinformatics approach could be considered as a rational platform for designing vaccines against such multi-stage parasites. Furthermore, it is proposed that this multi-epitope vaccine is served as a promising preventive anti-echinococcosis agent.

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Proteomic analysis of the excretory/secretory products and antigenic proteins of Echinococcus granulosus adult worms from infected dogs

Cited by 27 publications

References 39 publications

Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta

Identification of immunogenic proteins of the cysticercoid of Hymenolepis diminuta

Echinococcosis: Advances in the 21st Century

A multi-method and structure-based in silico vaccine designing against Echinococcus granulosus through investigating enolase protein

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