Proteomic analysis of the effects of simulated microgravity in human gastric mucosal cells

Lu, Sheng-Yu; Guo, Song; Chai, Shao-Bin; Yang, Jiaqi; Yuan, Yawei; Li, Hao; Yan, Hu; Zhang, Tao; Sun, Peng; Sun, Hongwei; Zhou, Jianan; Yang, Jianwu; Li, Zhengpeng; Cui, Yan

doi:10.1016/j.lssr.2021.10.001

Cited by 5 publications

(5 citation statements)

References 55 publications

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“…In addition, studies have revealed structural changes in the mucosa and other layers of the stomach and intestines, resulting in a risk of progressive accumulation of changes at the tissue level up to the formation of morphological signs of atrophy and a loss of muscle mass [12][13][14][15]. All these adverse effects threaten the health and performance of the crew and the overall success of the spaceflight mission [12,[16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents

Samoilenko,

Shishkina,

Antakova

et al. 2023

IJMS

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Smooth muscle tissue (SMT) is one of the main structural components of visceral organs, acting as a key factor in the development of adaptive and pathological conditions. Despite the crucial part of SMT in the gastrointestinal tract activity, the mechanisms of its gravisensitivity are still insufficiently studied. The study evaluated the content of smooth muscle actin (α-SMA) in the membranes of the gastric fundus and jejunum in C57BL/6N mice (30-day space flight), in Mongolian gerbils Meriones unguiculatus (12-day orbital flight) and after anti-orthostatic suspension according to E.R. Morey-Holton. A morphometric analysis of α-SMA in the muscularis externa of the stomach and jejunum of mice and Mongolian gerbils from space flight groups revealed a decreased area of the immunopositive regions, a fact indicating a weakening of the SMT functional activity. Gravisensitivity of the contractile structures of the digestive system may be due to changes in the myofilament structural components of the smooth myocytes or myofibroblast actin. A simulated antiorthostatic suspension revealed no significant changes in the content of the α-SMA expression level, a fact supporting an alteration in the functional properties of the muscularis externa of the digestive hollow organs under weightless environment. The data obtained contribute to the novel mechanisms of the SMT contractile apparatus remodeling during orbital flights and can be used to improve preventive measures in space biomedicine.

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Section: Introductionmentioning

confidence: 99%

Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents

Samoilenko,

Shishkina,

Antakova

et al. 2023

IJMS

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“…Функционирование многих систем организма является гравитационно зависимым, как в условиях невесомости, так и в модельных экспериментах. Так в работах авторов были показаны значимые изменения со стороны сердечно-сосудистой [10,15], иммунной [16,19], пищеварительной [1,3,12] систем, а также сенсомоторного и опорнодвигательного аппаратов [9,17]. Грависенситивность проявляется также на тканевом, клеточном и молекулярном уровнях [1,3,5,12,18].…”

Section: Introductionunclassified

“…Так в работах авторов были показаны значимые изменения со стороны сердечно-сосудистой [10,15], иммунной [16,19], пищеварительной [1,3,12] систем, а также сенсомоторного и опорнодвигательного аппаратов [9,17]. Грависенситивность проявляется также на тканевом, клеточном и молекулярном уровнях [1,3,5,12,18]. Тучные клетки (ТК) являются повсеместно распространенными многофункциональными клетками соединительной ткани, активно вовлеченными в развитие как адаптивных, так и патологических процессов [1,8].…”

Section: Introductionunclassified

“…Немаловажную роль ТК играют и в развитии патофизиологических механизмов многих заболеваний, включая аллергию, астму, анафилаксию, онкогенез, расстройства сердечно-сосудистой и пищеварительной систем [8,9,11]. Так изменение барьерного баланса слизистой оболоч-ки желудка, ремоделирование волокнистого компонента соединительной ткани, снижение сократительной способности гладких миоцитов под воздействием факторов измененной гравитации могут привести к серьезным последствиям, таким, как развитие гастрита и язвы желудка, нарушению эвакуаторной функции [3,6,12,18]. Принимая во внимание роль ТК в регуляции функций органов желудочно-кишечного тракта (ЖКТ), их изучение в космической гастроэнтерологии представляет несомненную актуальность.…”

Section: Introductionunclassified

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Gastric mast cell population in rats under ground-simulated weightlessness conditions and in early readaptation period

Zhukov

Alexeeva

Шишкина

et al. 2023

Žurnal anatomii i gistopatologii

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The effect of altered gravity on the digestive system is one of the challenging issues in space biology and medicine. The study of the effect of weightlessness on the mast cell (MC) functioning in the gastrointestinal organs is determined by their multifunctionality and participation in the developing adaptive and pathological processes. The aim of the study was to investigate the morphofunctional state of MCs in the stomach membranes of Wistar rats during 14 days of ground-based simulated weightlessness conditions and in a 3-day recovery period. Material and methods. The experiment involved 3 groups of Wistar rats: control, experimental – exposed to antiorthostatic suspension – and a 3-day-recovery group, 7 animals each, respectively. Identification of MCs was carried out using May-Grunwald staining with additional staining with Giemsa solution and immunohistochemical tryptase detection. The results obtained were statistically processed with SPSS 13 using parametric and non-parametric criteria. Results. A significantly increased number of metachromatic MC population was found only in the mucosa in animals of the antiorthostatic suspension and 3-day-recovery groups compared to the vivarium control. There was a sharp decrease in degranulating forms of MCs in the submucosalcoat in animals of the 3-day-recovery group compared to the control. A significantly increased number of degranulating forms of MCs was revealed in the muscle membrane in animals of the antiorthostatic suspension group. Immunohistochemical staining of MCs with antibodies to tryptase, in contrast to staining for metachromasia, allowed identifying a significant representation of tryptase-positive cells in the gastric mucosa in animals of the 3-day-recovery group. Conclusion. The results obtained support gravisensitivity of certain structural components of the stomach, and also evidence MC and their secretome participation in the developing adaptive responses to the effects of altered gravity both at the cellular and tissue levels.

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“…After a 24 h incubation in a sim-µG, a human seminoma cell line exhibited markers of oxidative stress, such as enlarged mitochondria and increased intracellular ROS, protein and lipid oxidation, and SOD [10]. Human gastric mucosal cells showed signs of stress and inflammation following 3 days of sim-µG, whereas oxidative stress on the same cells was detected after 7 days of exposure to microgravity [11]. Our own studies on fetal human NSC-derived oligodendrocyte progenitors (OLPs) revealed increased mitochondrial respiration and increased glycolysis after 24 h of exposure to sim-µG.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Profiling of the Secretome from Human Neural Stem Cells Flown into Space

Biancotti,

Espinosa-Jeffrey

2023

Bioengineering

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The change in gravitational force has a significant effect on biological tissues and the entire organism. As with any alteration in the environment, microgravity (µG) produces modifications in the system inducing adaptation to the new condition. In this study, we analyzed the effect of µG on neural stem cells (NSCs) following a space flight to the International Space Station (ISS). After 3 days in space, analysis of the metabolome in culture medium revealed increased glycolysis with augmented pyruvate and glycerate levels, and activated catabolism of branched-chain amino acids (BCAA) and glutamine. NSCs flown into space (SPC-NSCs) also showed increased synthesis of NADH and formation of polyamine spermidine when compared to ground controls (GC-NSCs). Overall, the space environment appears to increase energy demands in response to the µG setting.

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Proteomic analysis of the effects of simulated microgravity in human gastric mucosal cells

Cited by 5 publications

References 55 publications

Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents

Smooth Muscle Actin as a Criterion for Gravisensitivity of Stomach and Jejunum in Laboratory Rodents

Gastric mast cell population in rats under ground-simulated weightlessness conditions and in early readaptation period

Metabolomic Profiling of the Secretome from Human Neural Stem Cells Flown into Space

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