Proteomic analysis of the effect of heat stress on hexaploid wheat grain: Characterization of heat‐responsive proteins from total endosperm

Abstract

High temperatures during grain filling have been reported to be one of the factors that can affect the dough properties and quality characteristics of wheat. Responses to high temperature have been related to changes in protein composition at both quantitative and qualitative levels. The present study was conducted to determine the influence of high temperature during grain filling on the protein composition of bread wheat evaluated by proteomic tools. Plants were grown in the field and transferred to cabinets soon after flowering. They were subjected to two thermal regimes 18 degrees C/10 degrees C (day/night) and 34 degrees C/10 degrees C. Total proteins were extracted from control grains and treated plants at three different post-anthesis stages. The proteins were separated by two-dimensional gel electrophoresis and analysed by Melanie 3 software. Of the total number of mature wheat grain proteins, 37 were identified as significantly changed by heat treatment. Analysis by matrix-assisted laser desorption/ionization mass spectrometry and tandem mass spectrometry coupled with database searching allowed the characterization of 25 heat-induced proteins and only one heat-decreased protein spot. To learn more about the function of the identified proteins, we examined their expression during treatment.

A proteomic approach in analyzing heat‐responsive proteins in rice leaves

“…Although a group of HSPs was highly up-regulated by heat stress in this study, the induction of sHSPs suggests that they play a crucial and precise role against heat stress by reestablishing normal protein confirmations and thus, cellular homeostasis. The up-regulation or induction of sHSPs has also been noticed in several heat stress related proteomic analyses, which suggest that sHSPs may be used as a biomarker for heat stress [3,11,13]. Earlier, we showed that the overexpression of a rice chloroplast sHSP in Escherichia coli conferred better tolerance not only to heat stress, but it also provided tolerance to oxidative stress [29].…”

“…The current status of rice proteomic analysis has been reviewed recently [10], but there still are no reports regarding the effects of heat stress on rice leaves. Thus far, there have been only a few proteomic studies regarding heat stress in plants [3,5,[11][12][13]. To the best of our knowledge, only one proteomic analysis has been carried out during the caryopsis development of rice under heat stress [3].…”

Analysis of gluten proteins composition during grain filling in two durum wheat cultivars submitted to two water regimes

“…This increase was already reported in response to environmental factors, as high temperature (Majoul et al, 2003;Dupont et al, 2006aDupont et al, , 2006b). …”

Plant N Fluxes and Modulation by Nitrogen, Heat and Water Stresses: A Review Based on Comparison of Legumes and Non Legume Plants

“…In pea, the final level of vicilin storage proteins was higher under heat stress (Bourgeois et al, 2009). High temperature during seed filling also affects the accumulation level of stress/defense proteins, like heat shock proteins (Bourgeois et al, 2009;Hurkman et al, 2009;Majoul et al, 2003). The magnitude in which a heat stress during seed filling period affects seed quality depends on the heat tolerance of the genotype, the intensity and the timing of heat stress (Stone & Nicolas, 1996;Passarella et al, 2002;Spiertz et al, 2006).…”