Proteomic analysis of the compatible interaction between Vitis vinifera and Plasmopara viticola

Milli, Alberto; Cecconi, Daniela; Bortesi, Luisa; Persi, A.; Rinalducci, Sara; Zamboni, Anita; Zoccatelli, Gianni; Lovato, Arianna; Zolla, Lello; Polverari, Annalisa

doi:10.1016/j.jprot.2011.11.006

Cited by 57 publications

(42 citation statements)

References 71 publications

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“…Although the interactions between plant pathogens and their hosts have been studied using classical biochemical and molecular biological approaches [33][34][35][36][37][38], proteomics is an effective methodology that provides global information on various cellular protein networks [39,40]. In this study, we obtained a dynamic overview of the altered protein expression in strawberry leaves responding to C. fragariae infection.…”

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae

Fang

Chen

Xin

et al. 2012

Journal of Proteomics

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Section: Discussionmentioning

confidence: 99%

Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae

Fang

Chen

Xin

et al. 2012

Journal of Proteomics

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“…The availability of high-throughput analysis methods and a high-quality draft of the grapevine genome sequence (1) have led to the recent application of next-generation sequencing to investigate grape berry development and pathogen infection, although few proteomic studies are available. Upon infection, plants dramatically change at the molecular and biochemical levels as reflected by global transcriptome (2)(3)(4), proteome (5,6), and metabolome (7) system analyses. These studies demonstrate the importance of the complex modulation of plant metabolism and defense responses during pathogen infection.…”

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confidence: 99%

Modulation of Protein Phosphorylation, N-Glycosylation and Lys-Acetylation in Grape (Vitis vinifera) Mesocarp and Exocarp Owing to Lobesia botrana Infection

Melo‐Braga

Verano‐Braga

León

et al. 2012

Molecular & Cellular Proteomics

111

112

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Grapevine (Vitis vinifera) is an economically important fruit crop that is subject to many types of insect and pathogen attack. To better elucidate the plant response to Lobesia botrana pathogen infection, we initiated a global comparative proteomic study monitoring steady-state protein expression as well as changes in N-glycosylation, phosphorylation, and Lys-acetylation in control and infected mesocarp and exocarp from V. vinifera cv Italia. A multi-parallel, large-scale proteomic approach employing iTRAQ labeling prior to three peptide enrichment techniques followed by tandem mass spectrometry led to the identification of a total of 3059 proteins, 1135 phosphorylation sites, 323 N-linked glycosylation sites and 138 Lysacetylation sites. Of these, we could identify changes in abundance of 899 proteins. The occupancy of 110 phosphorylation sites, 10 N-glycosylation sites and 20 Lysacetylation sites differentially changed during L. botrana infection. Sequence consensus analysis for phosphorylation sites showed eight significant motifs, two of which containing up-regulated phosphopeptides (X-G-S-X and S-X-X-D) and two containing down-regulated phosphopeptides (R-X-X-S and S-D-X-E) in response to

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“…Grapevine plants (cv pinot noir) were grown in vitro (27°C, 16-hour photoperiod, 50 μE/m 2 /s) as previously described [5]. The Pv inoculum was collected from infected field leaves and used for several artificial infection cycles on surface-sterilized leaves before the final inoculum was stored as sporangia at -20°C.…”

Section: Methodsmentioning

confidence: 99%

“…The extraction of plant proteins was performed as previously described [5], with minor modifications, starting with both field- and in vitro-cultivated leaves. Briefly, infected and uninfected leaves were ground under liquid nitrogen in the presence of a protease inhibitor cocktail (1× Complete Tablet; Roche Molecular Biochemicals, Mannheim, Germany) and 5% PVPP (polyvinylpolypyrrolidone) to remove phenolic compounds.…”

Section: Methodsmentioning

confidence: 99%

“…Indeed, it has recently been demonstrated that Pv induces the expression of pathogenesis-related proteins (PRs) in vine-infected leaves [5]. The objective of the present work was to verify by a proteomic approach whether the allergenic reactions experienced by a farmer working in a vineyard infected by Pv were elicited by PRs expressed upon infection or by allergens present in Pv.…”

Section: Introductionmentioning

confidence: 97%

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Grapevine Downy Mildew <b><i>Plasmopara viticola</i></b> Infection Elicits the Expression of Allergenic Pathogenesis-Related Proteins

Rossin

Villalta

Martelli

et al. 2015

Int Arch Allergy Immunol

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Background: Downy mildews are a group of microorganisms belonging to the Chromista kingdom that can infect specific plants. When growing on plant tissues these microbes can elicit the expression of pathogenesis-related proteins (PRs), a group of stress-induced proteins frequently described as allergens in many plant species. Our aim was to verify by a proteomic approach whether the allergic reactions experienced by a farmer working in a vineyard infected by Plasmopara viticola (Pv), the etiological agent of downy mildew, are elicited by PRs expressed by the grapevine upon infection or by allergens present in Pv. Methods: A skin prick test and prick-to-prick test with infected field grapevine leaves and control leaves were carried out. Field leaves and ad hoc Pv-inoculated leaves were compared by SDS-PAGE and IgE-immunoblotting with extracts from control leaves and Pv sporangia. IgE-binding proteins were further separated by two-dimensional electrophoresis and the positive spots analyzed by nanoHPLC-Chip and tandem mass spectrometry (MS/MS) for identification. Results: Only infected leaves showed IgE-binding protein bands at 42 and 36 kDa. This agreed with the positive skin prick test experienced by the patient only with the infected leaves extract. Two-dimensional electrophoresis followed by MS/MS analysis led to the identification of PR-2 (β-1,3-glucanase) and harpin-binding protein 1 as putative allergens, the latter having never been reported before. Conclusion: The results indicate that Pv infection might represent a new source of plant allergens.

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Proteomic analysis of the compatible interaction between Vitis vinifera and Plasmopara viticola

Cited by 57 publications

References 71 publications

Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae

Proteomic analysis of strawberry leaves infected with Colletotrichum fragariae

Modulation of Protein Phosphorylation, N-Glycosylation and Lys-Acetylation in Grape (Vitis vinifera) Mesocarp and Exocarp Owing to Lobesia botrana Infection

Grapevine Downy Mildew <b><i>Plasmopara viticola</i></b> Infection Elicits the Expression of Allergenic Pathogenesis-Related Proteins

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