Proteomic analysis of synovial fluid: insight into the pathogenesis of knee osteoarthritis

Abstract

Purpose We conducted a proteomic analysis of synovial fluid (SF) to identify differentially expressed proteins and analyse their correlation with osteoarthritis (OA) severity. Our primary purpose was to gain insight into the pathogenesis of OA. Methods SF samples were acquired from 12 knee OA patients and 12 non-OA controls (ten had a meniscus injury, two had a discoid meniscus and all exhibited intact articular cartilage) and sequentially subjected to two-dimensional electrophoresis (2-DE). The radiographic grading of knee OA was performed using the Kellgren-Lawrence criteria. Differentially expressed proteins were identified by matrixassisted laser desorption/ionisation time-of-flight/time-offlight mass spectrometry (MALDI-TOF/TOF MS). Proteins of interest identified from SF were detected using an enzyme-linked immunosorbent assay (ELISA). Results A total of 31 protein spots showed significant differences (p<0.05) between the sample groups; 25 of the 31 spots (80.6 %) were identified as proteins of interest. Among them 20 corresponded to up-regulation and five to down-regulation in OA samples. HLA-DR was one of the proteins up-regulated, which was confirmed by ELISA. Conclusions These observations have implications in delineating the protein expression underlying the pathogenesis of OA and facilitate further elucidation of molecular mechanisms involved in disease progression. Substantial alterations of the protein profile in SF may be associated with OA severity.

Cartilage Quality (dGEMRIC Index) Following Knee Joint Distraction or High Tibial Osteotomy

“…The 1-year results of this subcohort are fully in line with the previously published 1-year results of the entire cohorts from both original RCTs. 21,31 One year after KJD, a statistically significant increase in medial, minimal, and mean JSW was found, this increase was still significant after 2 years. A statistically significant increase in medial and minimal JSW was found after 1 year in the HTO group, which also sustained at 2 years.…”

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1&alpha;,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

“…Reportedly ESWT ameliorated experimental osteoarthritic cartilage damage and alter angiogenesis pattern [15]. The preliminary proteomic data revealed abundant significant proteins that warrant further characterization [16][17][18]. These proteins of interest were reported to participate in the cellular response to physical stress, calcium homeostasis, chemotaxis and lipid oxidative stress in several tissue types under pathological contexts.…”

Exogenous expression ofIL-1RaandTGF-β1promotes in vivo repair in experimental rabbit osteoarthritis

“…The score for the blank control group was markedly higher than for the double transfection group and single transfection groups (all p < 0.05), and the grade of double transfection group was also higher than that of the single transfection groups (p < 0.05). (2) Vascellum invades the tidemark line (2) Fissure reaches the radiation layer (3) Obvious decrease (3) Severe decrease (3) Cartilage layer decreases (4) Completely disappeared (4) Scores given in parentheses.…”

“…Osteoarthritis (OA) is a heterogeneous disease primarily caused by an imbalance in repair or insufficient self-repair of injured synovial joint tissue (1,2). OA affects more than 15% of the global population, imposing heavy burdens on healthcare services worldwide (1,3,4).…”