Proteomic analysis of sugar beet apomictic monosomic addition line M14

Li, Haiying; Cao, Hongxiang; Wang, Yuguang; Pang, Qiuying; Ma, Castello; Chen, Sixue

doi:10.1016/j.jprot.2009.09.012

Cited by 23 publications

(15 citation statements)

References 38 publications

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“…Transcriptional and translational proteins are known to be affected by long-term salt stress [13][14][15][16][70][71][72], here we showed phosphorylation of these proteins in the shortterm salt-stress responses.…”

Section: Transcription-related Proteins and Protein Synthesissupporting

confidence: 62%

“…Sugar beet monosomic addition line M14 was obtained from the intercross between Beta vulgaris L. and B. corolliflora Zoss. It contains the B. vulgaris L. genome with the addition of chromosome 9 of B. corolliflora Zoss [13]. Our previous studies have shown M14 can tolerate 500 mM NaCl treatment for seven days, under which 38 unique proteins from leaves and 29 unique proteins from roots exhibited significant changes.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative proteomics and phosphoproteomics of sugar beet monosomic addition line M14 in response to salt stress

Yu¹,

Koh

et al. 2016

Journal of Proteomics

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Section: Transcription-related Proteins and Protein Synthesissupporting

confidence: 62%

Section: Introductionmentioning

confidence: 99%

Quantitative proteomics and phosphoproteomics of sugar beet monosomic addition line M14 in response to salt stress

Yu¹,

Koh

et al. 2016

Journal of Proteomics

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“…Sugar beet monosomic addition line M14 was acquired from the hybridization between Beta vulgaris L. and Beta corolliflora Zoss [24]. The M14 line has shown characteristics of apomixis and tolerance to abiotic stress [25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress

Wang

et al. 2017

IJMS

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The sugar beet monosomic addition line M14 is a unique germplasm that contains genetic materials from Beta vulgaris L. and Beta corolliflora Zoss, and shows tolerance to salt stress. Our study focuses on exploring the molecular mechanism of the salt tolerance of the sugar beet M14. In order to identify differentially expressed genes in M14 under salt stress, a subtractive cDNA library was generated by suppression subtractive hybridization (SSH). A total of 36 unique sequences were identified in the library and their putative functions were analyzed. One of the genes, S-adenosylmethionine synthetase (SAMS), is the key enzyme involved in the biosynthesis of S-adenosylmethionine (SAM), a precursor of polyamines. To determine the potential role of SAMS in salt tolerance, we isolated BvM14-SAMS2 from the salt-tolerant sugar beet M14. The expression of BvM14-SAMS2 in leaves and roots was greatly induced by salt stress. Overexpression of BvM14-SAMS2 in Arabidopsis resulted in enhanced salt and H2O2 tolerance. Furthermore, we obtained a knock-down T-DNA insertion mutant of AtSAMS3, which shares the highest homology with BvM14-SAMS2. Interestingly, the mutant atsam3 showed sensitivity to salt and H2O2 stress. We also found that the antioxidant system and polyamine metabolism play an important role in salt and H2O2 tolerance in the BvM14-SAMS2-overexpressed plants. To our knowledge, the function of the sugar beet SAMS has not been reported before. Our results have provided new insights into SAMS functions in sugar beet.

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“…Li et al ( 2009 ) reported a comparative proteomic and transcriptomic study of the sexual and apomictic processes in sugar beet. The cDNA libraries were constructed using SSH with the apomictic monosomic addition line M14 as the tester and B. vulgaris as the driver.…”

Section: Transcriptomics Studies In Sugar Beetmentioning

confidence: 99%

OMICS Technologies and Applications in Sugar Beet

Zhang

Nan

2016

Front. Plant Sci.

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Sugar beet is a species of the Chenopodiaceae family. It is an important sugar crop that supplies approximately 35% of the sugar in the world. Sugar beet M14 line is a unique germplasm that contains genetic materials from Beta vulgaris L. and Beta corolliflora Zoss. And exhibits tolerance to salt stress. In this review, we have summarized OMICS technologies and applications in sugar beet including M14 for identification of novel genes, proteins related to biotic and abiotic stresses, apomixes and metabolites related to energy and food. An OMICS overview for the discovery of novel genes, proteins and metabolites in sugar beet has helped us understand the complex mechanisms underlying many processes such as apomixes, tolerance to biotic and abiotic stresses. The knowledge gained is valuable for improving the tolerance of sugar beet and other crops to biotic and abiotic stresses as well as for enhancing the yield of sugar beet for energy and food production.

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Proteomic analysis of sugar beet apomictic monosomic addition line M14

Cited by 23 publications

References 38 publications

Quantitative proteomics and phosphoproteomics of sugar beet monosomic addition line M14 in response to salt stress

Quantitative proteomics and phosphoproteomics of sugar beet monosomic addition line M14 in response to salt stress

Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress

OMICS Technologies and Applications in Sugar Beet

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