Proteomic analysis of soybean root including hypocotyl during recovery from drought stress

Khan, Mudassar Nawaz; Komatsu, Setsuko

doi:10.1016/j.jprot.2016.06.006

Cited by 46 publications

(23 citation statements)

References 55 publications

(61 reference statements)

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“…Both proteins were upregulated, peaking after 24–48 h of drought stress and returning to control levels in the roots and leaves of recovering plants. These changes also occur in soybean undergoing drought stress and during post-drought recovery51. This suggests that L-ascorbate peroxidase plays an important role in cell survival and drought tolerance.…”

Section: Discussionmentioning

confidence: 90%

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Bian

Deng

Xing

et al. 2017

Sci Rep

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In this study, we performed the first integrated physiological and proteomic analysis of the response to drought and recovery from drought, using Brachypodium distachyon L. Roots and leaves. Drought stress resulted in leaves curling, root tips becoming darker in color and significant changes in some physiological parameters. Two-dimensional difference gel electrophoresis (2D-DIGE) identified 78 and 98 differentially accumulated protein (DAP) spots representing 68 and 73 unique proteins responding to drought stress and/or recovery in roots and leaves, respectively. Differences between the root and leaf proteome were most marked for photosynthesis, energy metabolism, and protein metabolism. In particular, some DAPs involved in energy and protein metabolism had contrasting accumulation patterns in roots and leaves. Protein-protein interaction (PPI) analysis of roots and leaves revealed complex protein interaction networks that can generate synergistic responses to drought stress and during recovery from drought. Transcript analysis using quantitative real-time polymerase chain reaction (qRT-PCR) validated the differential expression of key proteins involved in the PPI network. Our integrated physiological and proteomic analysis provides evidence for a synergistic network involved in responses to drought and active during recovery from drought, in Brachypodium roots and leaves.Plants encounter a variety of biotic and abiotic stresses during growth 1 . These stresses unbalance cellular homeostasis and lead to morphological, physiological, and molecular changes 2 . These changes have a negative impact on survival and biomass production, and can reduce final yield by up to 82% 3 . Global warming and climate change may also be exacerbating the effects of abiotic stresses on crop production in many areas of the world today. Previous reports have suggested that a temperature increase of 1 °C can produce a decrease in yield of up to 10% 4 . Drought in particular, severely impairs plant growth and development and limits crop productivity more than any other environmental factor 5,6 . As the climate continues to change, drought may become a more frequent and cause severe problem 7 . Drought stress induces a range of physiological and biochemical responses in plants. Under drought conditions, the plant root cap produces abscisic acid (ABA), which mediates stomatal closure. This in turn, suppresses cell growth, photosynthetic efficiency, and respiration [8][9][10] . Recently, ABA and stomata have been implicated in the regulation of systemic responses to abiotic stresses 11 . In addition, plants generate toxic ions and reactive oxygen species (ROS) that can decrease enzyme activities and damage essential proteins 12 . The generation of ROS occurs early in the response to water-stress, and ROS are key secondary messengers triggering subsequent defensive measures in plants 1,[13][14][15] . Plants have developed a sophisticated and elaborate system for scavenging high levels of ROS using antioxidant enzymes that include superoxid...

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Section: Discussionmentioning

confidence: 90%

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Bian

Deng

Xing

et al. 2017

Sci Rep

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show abstract

“…Moreover, CK levels and drought tolerance were enhanced in rice plants overexpressing the isopentyl transferase ( IPT ) gene, which corresponded with the upregulation of various BR-related biosynthesis genes ( DWF4 , DWF5 , and HYD1 ) and BR signaling genes ( BRI1 , BZR1 , BAK1 , SERK1 , and BRH1 ) 73 . Previous studies have shown that 2-oxoglutarate Fe(II)-dependent oxygenase superfamily proteins are involved in the ethylene metabolism process and plant defense response to drought 74 . Other studies have shown that hormone crosstalk plays an important role via interactions with abiotic stress-related pathways in soybean 53,75 .…”

Section: Discussionmentioning

confidence: 99%

Genome-wide transcriptional profiling for elucidating the effects of brassinosteroids on Glycine max during early vegetative development

Song

Chen

Yao

et al. 2019

Sci Rep

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Soybean is a widely grown grain legume and one of the most important economic crop species. Brassinosteroids play a crucial role in plant vegetative growth and reproductive development. However, it remains unclear how BRs regulate the developmental processes in soybean, and the molecular mechanism underlying soybean early development is largely unexplored. In this study, we first characterized how soybean early vegetative growth was specifically regulated by the BR biosynthesis inhibitor propiconazole; this characterization included shortened root and shoot lengths, reduced leaf area, and decreased chlorophyll content. In addition, the growth inhibition induced by Pcz could be rescued by exogenous brassinolide application. The RNA-seq technique was employed to investigate the BR regulatory networks during soybean early vegetative development. Identification and analysis of differentially expressed genes indicated that BRs orchestrate a wide range of cellular activities and biological processes in soybean under various BR concentrations. The regulatory networks between BRs and multiple hormones or stress-related pathways were investigated. The results provide a comprehensive view of the physiological functions of BRs and new insights into the molecular mechanisms at the transcriptional level of BR regulation of soybean early development.

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“…Even under water restriction, this adjustment allows the plant to keep the stomata partially open to assimilate CO 2 and continue the processes of cell elongation and plant growth (Araújo and Deminicis, 2009;Monteiro et al, 2014). In order to allow osmotic adjustment, tolerant plants accumulate osmotically active compounds such as polyamines, glycine, organic acids, calcium, potassium, chloride ions, proline, sugars, and polyols, particularly sucrose (Cvikrová et al, 2013;Khan and Komatsu, 2016;Batista et al, 2018). Along with high efficiency in osmotic adjustment and water use, the ability to maintain photosynthetically active structures is also important for the selection of soybean cultivars with double tolerance to stresses caused by water deficit and high temperature.…”

Section: Introductionmentioning

confidence: 99%

Physiological tolerance to drought under high temperature in soybean cultivars

et al. 2019

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Soybean is one of the most economically important crops and has experienced adverse physiological and biochemical effects when subjected to drought stress and heat, resulting in lost productivity. Thus, the objective of this work was to evaluate the physiological, metabolic and growth responses of well-watered and drought-treated soybean cultivars under high temperature. The experimental design was set up in randomized blocks, in a factorial scheme with three soybean cultivars (7739 M, Anta 82 and Desafio) and two water levels (100% and 40% field capacity). The experiment was conducted in a controlled growth chamber with a gradual rise in temperature at 41°C for 5 hours daily. Morpho-physiological and metabolic analyses were performed 12 days after the treatments imposition. The parameters of water and osmotic potentials, relative water content, photosynthetic rate, stomatal conductance, transpiratory rate, electron flux for the carboxylation and oxygenation of RuBisCO were decreased for all cultivars under water deficit and high temperature. The results showed that the photorespiration and the rate of electrolyte leakage were increased as well. These results showed that these physiological behaviors are standard for soybean plants under water deficit, regardless of cultivars. The cultivars 7739 M and Desafio showed lower performance than the cultivar Anta 82 for the parameters of total electron flow and effective quantum yield of PS II. The 7739 M and Anta 82 were the only cultivars to show increased nonphotochemical quenching dissipation and total soluble sugar content, respectively, under stress conditions. Desafio cultivar demonstrated greater physiological and growth traits stability, which could potentially indicate double tolerance to these stresses.

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Proteomic analysis of soybean root including hypocotyl during recovery from drought stress

Cited by 46 publications

References 55 publications

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Integrated proteomic analysis of Brachypodium distachyon roots and leaves reveals a synergistic network in the response to drought stress and recovery

Genome-wide transcriptional profiling for elucidating the effects of brassinosteroids on Glycine max during early vegetative development

Physiological tolerance to drought under high temperature in soybean cultivars

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