Proteomic analysis of soybean hypocotyl during recovery after flooding stress

Khan, Mudassar Nawaz; Sakata, Katsumi; Komatsu, Setsuko

doi:10.1016/j.jprot.2015.03.020

Cited by 22 publications

(19 citation statements)

References 43 publications

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“…His finding indicated that reduction in growth was greater when flooding stress was applied at vegetative stage since the plants were not able to restore the physiological function to attain the optimal growth level after flooding. Khan et al (2015) identified that pyruvate kinase, nucleotidylyl transferase, and beta-ketoacyl reductase played as key roles in post-flooding recovery in soybean hypocotyl by promoting glycolysis for generation of ATP and regulation of secondary metabolic pathways. Mean of shoot/root ratio was lower in bean plants exposed to shallower soil water table, even if the value was statistically insignificant, masked by high variability in responses to the stress amongst individual plants (Table 5).…”

Section: Discussionmentioning

confidence: 99%

Cultivation of common bean (Phaseolus vulgaris L.) subjected to shallow water table at riparian wetland in South Sumatra, Indonesia

Susilawati¹,

Lakitan²

2019

Aust J Crop Sci

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Common bean is a susceptible vegetable to excessive water condition in soil. Meanwhile, flooding occurrence and soil water table are unpredictable at riparian wetlands. These circumstances make cultivation of common bean in riparian wetland challenging. A field experiment was conducted at post flooding period but soil water table was still less than 30 cm below soil surface during transitional period from wet to dry season, in May to August 2017. Site location was a paddy field at Sungai Selincah Village, within riparian wetland ecosystem in South Sumatra, Indonesia. The paddy field is characterized by alluvial soil and periodically flooded for 4-6 months during rainy season. Raised beds were constructed for setting up water table positions at 10, 15, and 20 cm below soil surface. Results of this study indicated that common bean (Phaseolus vulgaris L.) could tolerate soil water table at depth of 15 cm or deeper without significant decrease in growth and yield. However, soil water table at depth of 10 cm significantly reduced plant height, number of trifoliate leaves, diameter of canopy, shoot dry weight, root length, number of primary lateral roots, chlorophyll content index (CCI) during reproductive stage, total number of pods, and cumulative yield. In conclusion, it is possible to grow common bean at riparian wetland ecosystem as early as soil water table has subsided to 15 cm below soil surface during transitional period from wet to dry season.

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Section: Discussionmentioning

confidence: 99%

Cultivation of common bean (Phaseolus vulgaris L.) subjected to shallow water table at riparian wetland in South Sumatra, Indonesia

Susilawati¹,

Lakitan²

2019

Aust J Crop Sci

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“…Constant progress in the available technologies ena-Table 1 . Summary of the described studies of soybean stress response analyzed with high-throughput technologies focused on transcriptomic, miRNA and proteomic analyses Transcriptome studies miRNA studies Proteome studies Drought Le et al, 2012Chen et al, 2013Shin et al, 2015Tripathi et al, 2016Li et al, 2011-Flooding Nanjo et al, 2011aNanjo et al, 2010Nanjo et al, 2011bYin et al, 2014aKhan et al, 2015Yin & Komatsu, 2015 Cold stress -- Tian et al, 2015Salinity -Dong et al, 2013Sun et al, 2016Ma et al, 2012 Phosphate deficiency Zeng et al, 2015Wang et al 2016Xu et al, 2013Zenga et al, 2010Chen et al, 2011 Biotic stress Lanubile et al, 2015Li et al, 2012bYin et al, 2013 -High-throughput analyses of soybean stress response bles us to create gene expression atlases, which may present snapshots of the transcriptome profiles even for the whole life-cycles of plants. Moreover, expression profiling obtained by using high-throughput methods contributes to the development of molecular markers, finding genes responsible for the secondary metabolism and studying the evolution of organs in plant families.…”

Section: Transcriptomementioning

confidence: 99%

“…Post-flooding proteome responses in soybean hypocotyl were analyzed during the recovery period using a gel-free technique (Khan et al, 2015). 20 proteins, in common between the control and flooding-stressed soybeans that changed significantly in abundance during the post-flooding recovery have been identified using mass spectrometry analysis.…”

Section: Floodingmentioning

confidence: 99%

Deciphering soybean molecular stress response via high-throughput approach

et al. 2017

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As a result of thousands of years of agriculture, humans had created many crop varieties that became the basis of our daily diet, animal feed and also carry industrial application. Soybean is one of the most important crops worldwide and because of its high economic value the demand for soybean products is constantly growing. In Europe, due to unfavorable climate conditions, soybean cultivation is restricted and we are forced to rely on imported plant material. The development of agriculture requires continuous improvements in quality and yield of crop varieties under changing or adverse conditions, namely stresses. To achieve this goal we need to recognize and understand the molecular dependencies underlying plant stress responses. With the advent of new technologies in studies of plant transcriptomes and proteomes, now we have the tools necessary for fast and precise elucidation of desirable crop traits. Here, we present an overview of high-throughput techniques used to analyze soybean responses to different abiotic (drought, flooding, cold stress, salinity, phosphate deficiency) and biotic (infections by F. oxysporum, cyst nematode, SMV) stress conditions at the level of the transcriptome (mRNAs and miRNAs) and the proteome.

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“…60,61 It is identified that lipoxygenase and peroxidase were significantly changed at protein abundance in root. Lipoxygenase, which was slightly increased at protein abundance, was progressively down-regulated at mRNA expression level, and its activity decreased gradually during the postflooding recovery.…”

Section: Detecting Stress Response Proteinsmentioning

confidence: 99%

Proteomic Techniques and Management of Flooding Tolerance in Soybean

2015

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Climate change is considered a major threat to world agriculture and food security. To improve the agricultural productivity and sustainability, the development of high-yielding stress-tolerant, and climate-resilient crops is essential. Of the abiotic stresses, flooding stress is a very serious hazard because it markedly reduces plant growth and grain yield. Proteomic analyses indicate that the effects of flooding stress are not limited to oxygen deprivation but include many other factors. Although many flooding response mechanisms have been reported, flooding tolerance mechanisms have not been fully clarified for soybean. There were limitations in soybean materials, such as mutants and varieties, while they were abundant in rice and Arabidopsis. In this review, plant proteomic technologies are introduced and flooding tolerance mechanisms of soybeans are summarized to assist in the improvement of flooding tolerance in soybeans. This work will expedite transgenic or marker-assisted genetic enhancement studies in crops for developing high-yielding stress-tolerant lines or varieties under abiotic stress.

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Proteomic analysis of soybean hypocotyl during recovery after flooding stress

Cited by 22 publications

References 43 publications

Cultivation of common bean (Phaseolus vulgaris L.) subjected to shallow water table at riparian wetland in South Sumatra, Indonesia

Cultivation of common bean (Phaseolus vulgaris L.) subjected to shallow water table at riparian wetland in South Sumatra, Indonesia

Deciphering soybean molecular stress response via high-throughput approach

Proteomic Techniques and Management of Flooding Tolerance in Soybean

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