Proteomic analysis of soybean defense response induced by cotton worm (prodenia litura, fabricius) feeding

Abstract

BackgroundCotton worm is one of the main insects of soybean in southern China. Plants may acquire defense mechanisms that confer protection from predation by herbivores. Induced responses can lead to increased resistance against herbivores in many species. This study focuses on searching changed proteins in soybean defense response induced by cotton worm feeding.ResultsTen protein spots that are changed in abundance in response to cotton worm feeding were identified by Two-dimensional gel electrophoresis (2-DE). A total of 11 unique proteins from these spots were identified by MALDI-TOF MS. The mRNA and protein relative expression levels of most changed proteins were up-regulated. These proteins were mainly involved in physiological processes, including active oxygen removal, defense signal transduction, and metabolism regulation.ConclusionThis is the first proteomic analysis of the soybean defense response induced by cotton worm. The differentially expressed proteins could work together to play a major role in the induced defense response. PAL and SAMS were up-regulated at both the protein and mRNA levels. These genes can be strongest candidates for further functional research.

Characterization of proteins in soybean roots under flooding and drought stresses

“…The levels of S-adenosylmethionine synthetase have also been shown to increase in response to cold stress in rice (71), mechanical wounding in lima bean (72), salinity stress in barley (73), and as part of defense responses to cotton worm feeding in soybean (74). Furthermore, it was reported that the levels of S-adenosylmethionine synthetase are decreased in flooded soybean, indicating that this enzyme plays a central role in the response to flooding in soybean (13,14).…”

Transcriptome analysis of soybean lines reveals transcript diversity and genes involved in the response to common cutworm (Spodoptera litura Fabricius) feeding

“…In our previous research, 11 proteins were identified from the proteome of NN soybean leaves damaged by common cutworm feeding (Fan et al . ), of these proteins, serine/threonine kinase‐related protein and VSP α were also shown to have transcriptome changes. The correlation between transcripts and proteins was observed to be good on specific targets (Zulak et al .…”

“…We previously identified an induced resistance response caused by the feeding of common cutworms in a susceptible soybean line, and we detected 11 differently expressed proteins that may be involved in this induced defence response using proteomic approaches (Fan et al . ). However, the biochemical mechanisms and signalling pathways of the induced response in soybeans that leads to the decreased herbivore preference and/or performance are still poorly understood.…”

“…Insect-induced responses in soybean have only been studied for a small number of genes that are involved in secondary metabolism and octadecanoid signalling (Jouanin et al 1998;Marchetti et al 2000;Zhu-Salzman et al 2003;Wu et al 2008). We previously identified an induced resistance response caused by the feeding of common cutworms in a susceptible soybean line, and we detected 11 differently expressed proteins that may be involved in this induced defence response using proteomic approaches (Fan et al 2012). However, the biochemical mechanisms and signalling pathways of the induced response in soybeans that leads to the decreased herbivore preference and/or performance are still poorly understood.…”

Proteomic Analysis Reveals the Positive Roles of the Plant-Growth-Promoting Rhizobacterium NSY50 in the Response of Cucumber Roots to Fusarium oxysporum f. sp. cucumerinum Inoculation

“…Overexpression of suadea salsa SAMs gene promoted salt tolerance in transgenic tobacco (Qi et al, 2010). An increase in production of SAMs was observed in cold stressed rice (Cui et al, 2005), mechanically wounded Phaseolus lunatus (Arimura et al, 2002), salt-stressed barley (Witzel et al, 2009), and in response to cotton worm feeding in soybean (Fan et al, 2012). Hence, the remarkable expression of SAMs by NSY50 suggested that plant might promote SAM-dependant metabolism through synthesize more SAM to further enhance the synthesis of new proteins and stress tolerance.…”