Proteomic analysis of secreted saliva from Russian Wheat Aphid (Diuraphis noxia Kurd.) biotypes that differ in virulence to wheat

Nicholson, Scott J.; Hartson, Steven D.; Puterka, Gary J.

doi:10.1016/j.jprot.2012.01.031

Cited by 146 publications

(173 citation statements)

References 123 publications

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“…Feeding by Myzus persicae has been shown to induce accumulation of trehalose and this is thought to be critical in defence responses in Arabidopsis thaliana to aphids (Hodge et al , 2013). The saliva of D. noxia , A. pisum and Phlebotomus arabicus has been found to contain trehalase, which converts trehalose to two glucose molecules and it has been suggested that the role of trehalase in the saliva of the aphids is to disrupt the stress response signalling by the plant (Nicholson et al , 2012; Hodge et al , 2013). Another suggested role could be that the aphids are able to interfere with the sugar sensing pathway of the plant, manipulating it in their favour as has been suggested with plant pathogens (O'Hara et al , 2013).…”

Section: Discussionmentioning

confidence: 99%

Triticum monococcumlines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphidRhopalosiphum padi

et al. 2016

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Crop protection is an integral part of establishing food security, by protecting the yield potential of crops. Cereal aphids cause yield losses by direct damage and transmission of viruses. Some wild relatives of wheat show resistance to aphids but the mechanisms remain unresolved. In order to elucidate the location of the partial resistance to the bird cherry–oat aphid, Rhopalosiphum padi, in diploid wheat lines of Triticum monococcum, we conducted aphid performance studies using developmental bioassays and electrical penetration graphs, as well as metabolic profiling of partially resistant and susceptible lines. This demonstrated that the partial resistance is related to a delayed effect on the reproduction and development of R. padi. The observed partial resistance is phloem based and is shown by an increase in number of probes before the first phloem ingestion, a higher number and duration of salivation events without subsequent phloem feeding and a shorter time spent phloem feeding on plants with reduced susceptibility. Clear metabolic phenotypes separate partially resistant and susceptible lines, with the former having lower levels of the majority of primary metabolites, including total carbohydrates. A number of compounds were identified as being at different levels in the susceptible and partially resistant lines, with asparagine, octopamine and glycine betaine elevated in less susceptible lines without aphid infestation. In addition, two of those, asparagine and octopamine, as well as threonine, glutamine, succinate, trehalose, glycerol, guanosine and choline increased in response to infestation, accumulating in plant tissue localised close to aphid feeding after 24 h. There was no clear evidence of systemic plant response to aphid infestation.

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Section: Discussionmentioning

confidence: 99%

Triticum monococcumlines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphidRhopalosiphum padi

et al. 2016

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“…One such effect is chlorosis (Heng-Moss et al, 2003;Smith et al, 2005;Botha et al, 2006;Diaz-Montano et al, 2007;Goławska et al, 2010). Highly bioactive effector molecules in aphid salivary gland secretions may cause severe damage to infested plants (Cooper et al, 2010;Cooper et al, 2011;Nicholson et al, 2012;Rao et al, 2013). Although Z. mays is one of the most important model plants and a valuable crop, there has been no published assessment of aphid-evoked changes in the total chlorophyll concentration of seedlings representing a wide range of genotypes.…”

Section: Discussionmentioning

confidence: 99%

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

Sytykiewicz¹,

Czerniewicz²,

Sprawka³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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We measured the total chlorophyll (Chl a+b) content in seedling leaves of fifteen maize cultivars infested by two studied aphid species (oligophagous Rhopalosiphum padi L., monophagous Sitobion avenae F.) 7 and 14 days after the beginning of infestation, using a SPAD-502 chlorophyll meter. Chlorophyll loss was more severe in R. padi-infested than in S. avenae-infested plants. Chlorophyll depletion was greater after long-term (14 days) than after short-term aphid infestation in the investigated host systems. Seedlings of Złota Karłowa and Tasty Sweet were more damaged by aphid feeding; Ambrozja and Płomyk plants were less damaged by aphid feeding.K Ke ey y w wo or rd ds s: : Total chlorophyll (Chl a+b) content, SPAD units, maize, Rhopalosiphum padi, Sitobion avenae.

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“…Coagulable saliva forms salivary sheaths around the insect's stylets that help to stabilize and protect the stylets and may suppress plant defense responses to components of the watery saliva (Miles, 1999;Abdellatef et al, 2015). Watery saliva, which contains a mixture of amino acids, proteins, and digestive enzymes, assists in the digestion of plant material and helps suppress plant defense responses (Miles, 1968(Miles, , 1999Harmel et al, 2008;Carolan et al, 2009;Hogenhout and Bos, 2011;Nicholson et al, 2012;Elzinga and Jander, 2013). Thus, BPH saliva plays an important role in BPH feeding.…”

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confidence: 99%

A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice

Chen

et al. 2017

Plant Physiol.

107

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ORCID IDs: 0000-0002-5714-7586 (J.Z.); 0000-0002-3262-6134 (Y.L.).The brown planthopper (BPH) Nilaparvata lugens is one of the most destructive insect pests on rice (Oryza sativa) in Asia. After landing on plants, BPH rapidly accesses plant phloem and sucks the phloem sap through unknown mechanisms. We discovered a salivary endo-b-1,4-glucanase (NlEG1) that has endoglucanase activity with a maximal activity at pH 6 at 37°C and is secreted into rice plants by BPH. NlEG1 is highly expressed in the salivary glands and midgut. Silencing NlEG1 decreases the capacity of BPH to reach the phloem and reduces its food intake, mass, survival, and fecundity on rice plants. By contrast, NlEG1 silencing had only a small effect on the survival rate of BPH raised on artificial diet. Moreover, NlEG1 secreted by BPH did not elicit the production of the defense-related signal molecules salicylic acid, jasmonic acid, and jasmonoyl-isoleucine in rice, although wounding plus the application of the recombination protein NlEG1 did slightly enhance the levels of jasmonic acid and jasmonoyl-isoleucine in plants compared with the corresponding controls. These data suggest that NlEG1 enables the BPH's stylet to reach the phloem by degrading celluloses in plant cell walls, thereby functioning as an effector that overcomes the plant cell wall defense in rice.

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Proteomic analysis of secreted saliva from Russian Wheat Aphid (Diuraphis noxia Kurd.) biotypes that differ in virulence to wheat

Cited by 146 publications

References 123 publications

Triticum monococcumlines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphidRhopalosiphum padi

Triticum monococcumlines with distinct metabolic phenotypes and phloem-based partial resistance to the bird cherry-oat aphidRhopalosiphum padi

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

A Salivary Endo-β-1,4-Glucanase Acts as an Effector That Enables the Brown Planthopper to Feed on Rice

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