Proteomic analysis of salt-stressed tomato (Solanum lycopersicum) seedlings: effect of genotype and exogenous application of glycinebetaine

Abstract

An investigation aimed at a better understanding of the molecular adaptation mechanisms of salt stress was carried out in 7-d-old tomato Solanum lycopersicum (L.) Mill cultivars Patio and ‘F144’, using a proteomic approach. Total proteins were extracted from radicles and hypocotyls collected from both non-saline control and salt-stressed seedlings, and separated by two-dimensional gel electrophoresis. Liqud chromatography-electron spray ionization tandem mass spectrometry (LC-ESI-MS/MS) identified 23 salt stress response proteins, classified into six functional categories. The effect of exogenously applied glycinebetaine (GB) on the salt stress-induced inhibition of growth in tomato seedlings of cultivars Patio and ‘F144’ and on the protein profile was investigated. It was found that GB could alleviate the inhibition of tomato growth induced by salt stress through changing the expression abundance of six proteins in Patio and two proteins in ‘F144’ more than twice compared with salt-stressed seedlings. Furthermore, the interaction analysis based on computational bioinformatics reveals major regulating networks: photosystem II (PSII), Rubisco, and superoxide dismutase (SOD). The results suggest that it is likely that improvement of salt tolerance in tomato might be achieved through the application of exogenous compatible solutes, such as GB. Moreover, quantitative and qualitative analysis of the differentially expressed proteins of tomato under salt stress is an important step towards further elucidation of mechanisms of salt stress resistance.

Combined biotic and abiotic stress resistance in tomato

“…1 of IL2-3 coincides with QTLs identified in previous studies for enhanced germination, vegetative growth and fruit yield under salt stress (Foolad 1999;Villalta et al 2007). In this region, many candidate genes reside that are involved in the regulation of ion homeostasis, such as NHX3, NHX4 and SOS1 OlÍas et al 2009), and for redox homeostasis such as Cu/ZnSOD1 (Chen et al 2009). Notably, IL2-3 exhibited lower Na ?…”

Effects of Salt and Water Stress on Plant Growth and on Accumulation of Osmolytes and Antioxidant Compounds in Cherry Tomato

“…In this study, young tomato plants have been grown in the presence of increasing concentrations of NaCl, and a concentration-and time-dependent inhibition of vegetative growth has been observed, which was better shown by the reduction of the fresh weight of the plants; this was not been reported that this species lacks two enzymes required for GB biosynthesis (Goel et al, 2011). Nevertheless, exogenous application of GB to tomato plants improves their resistance to drought (Rezaei et al, 2012) and salinity (Chen et al, 2009;Heuer, 2003;Makela et al, 1998). On the other hand, there are some examples of plant species that under severe stress conditions can activate the simultaneous synthesis of several osmolytes to help alleviate the detrimental effects of stress, by contributing to osmotic adjustment and/or acting as 'osmoprotectants', as it has been recently discussed (Tipirdamaz et al, 2006;Gil et al, 2013).…”

Glycinebetaine improves salt tolerance in vinal (Prosopis ruscifolia Griesbach) seedlings

“…Genomic approaches promise extraordinary amounts of information about all osmotically induced genes (Cushman, 2001). Recently, Chen et al (2009) used a proteomic and bioinformatic approach to determine that improvement of salt tolerance in tomato might be achieved through the exogenous application of compatible solutes such as GB. The quantitative and qualitative analysis of differentially expressed tomato proteins under salt stress, based on computational bioinformatics, revealed three major regulating networks: photosystem II (PSII), Rubisco and SOD.…”