Proteomic Analysis of Rice Subjected to Low Light Stress and Overexpression of OsGAPB Increases the Stress Tolerance

Abstract

Background: Light provides the energy for photosynthesis and determines plant morphogenesis and development. Low light compromises photosynthetic efficiency and leads to crop yield loss. It remains unknown how rice responds to low light stress at a proteomic level. Results: In this study, the quantitative proteomic analysis with isobaric tags for relative and absolute quantitation (iTRAQ) was used and 1221 differentially expressed proteins (DEPs) were identified from wild type rice plants grown in control or low light condition (17% light intensity of control), respectively. Bioinformatic analysis of DEPs indicated low light remarkably affects the abundance of chloroplastic proteins. Specifically, the proteins involved in carbon fixation (Calvin cycle), electron transport, and ATPase complex are severely downregulated under low light. Furthermore, overexpression of the downregulated gene encoding rice β subunit of glyceraldehyde-3-phosphate dehydrogenase (OsGAPB), an enzyme in Calvin cycle, significantly increased the CO 2 assimilation rate, chlorophyll content and fresh weight under low light conditions but have no obvious effect on rice growth and development under control light. Conclusion: Our results revealed that low light stress on vegetative stage of rice inhibits photosynthesis possibly by decreasing the photosynthetic proteins and OsGAPB gene is a good candidate for manipulating rice tolerance to low light stress.

Overexpression of cytoplasmicSolanum tuberosumGlyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene improves PSII efficiency and alleviates salinity stress inArabidopsis.

“…Reduced electrolyte leakage, increased stomatal conductance, lower MDA accumulation, and enhanced proline accumulation in D43-NaCl plants (Figure 3A-D) was in accordance with several previous findings (Sreenivasulu et al 2000;Puyang et al 2015;Alavilli et al 2016;Blankenagel et al 2018). A recent study on rice demonstrated that expression of the OsGAPB gene increased the CO 2 assimilation rate and chlorophyll content in rice subjected to low or light stress (Liu et al 2020). Similarly, a recent study on melatonin-treated pepper plants demonstrated improved root and shoot biomass, higher accumulation of chlorophylls, and improved fruit yield (Kaya et al 2020).…”

Physiological mechanism of strigolactone enhancing tolerance to low light stress in cucumber seedlings

“…2 ). Therefore, the reduction in Pn in plants treated with low light is mainly attributed to nonstomatal limitation, including limited carbon assimilation or poor performance of the photosynthetic apparatus, while GR24 improves photosynthetic efficiency by reducing nonstomatal limitation [ 45 ]. In addition, the values of Fv/Fm, ΦPSII, ΦNPQ, qP and NPQ were significantly reduced under low light stress.…”

“…2). Therefore, the reduction in Pn in plants treated with low light is mainly attributed to nonstomatal limitation, including limited carbon assimilation or poor performance of the photosynthetic apparatus, while GR24 improves photosynthetic efficiency by reducing nonstomatal limitation [45]. In addition, the values of Fv/Fm, ΦPSII, ΦNPQ, qP and NPQ were significantly reduced under low light 9 Effects of GR24 treatment on AsA(A), DAsA(B), and AsA + DAsA contents (C), the AsA/DAsA ratio (D), GSH(E), GSSG(F), and GSH + GSSG(G) contents and the GSH/GSSG ratio (H) in cucumber seedlings under low light stress.…”

Understanding Omics Driven Plant Improvement and de novo Crop Domestication: Some Examples

“…The enhanced level of redox proteins, stress and defense related proteins, calcium ion regulation proteins, signaling G-protein and RNA metabolism related proteins were induced in phloem sap study. Recently, proteomics study revealed that low light stress obstructs carbon fixation and OsGAPB overexpression augment tolerance to low light stress conceivably by increasing CO 2 assimilation and chlorophyll accumulation in rice (Liu et al, 2020). Interestingly, simultaneous upregulation of both biotic and abiotic stress responsive protein has been observed during bacterial blight infection in rice, which indicate the activation of common pathway (Kumar et al, 2015).…”