Proteomic analysis of responses of a new probiotic bacterium Lactobacillus casei Zhang to low acid stress

Wu, Rina; Zhang, Wenyi; Sun, Tingting; Wu, Junrui; Yue, Xiqing; Meng, He; Zhang, Heping

doi:10.1016/j.ijfoodmicro.2011.04.003

Cited by 91 publications

(80 citation statements)

References 38 publications

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“…Koskenniemi et al (2011) hypothesized that L. rhamnosus GG might utilize Nacetylglucosamine as an energy source under bile stress. Wu et al also observed an increase in the abundance of NagA and NagB in response to low pH (Wu et al, 2011) and NagA in response to bile (Wu et al, 2010) in L. casei Zhang. In contrast, Hamon et al (2012) observed lower abundance of NagB in most L. casei strains.…”

Section: Cell Envelope-related Functionsmentioning

confidence: 86%

Proteomic and transcriptomic analysis of the response to bile stress of Lactobacillus casei BL23

Alcántara

Zúñiga

2012

Microbiology

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Lactobacillus casei is a lactic acid bacterium commonly found in the gastrointestinal tract of animals, and some strains are used as probiotics. The ability of probiotic strains to survive the passage through the gastrointestinal tract is considered a key factor for their probiotic action. Therefore, tolerance to bile salts is a desirable feature for probiotic strains. In this study we have characterized the response of L. casei BL23 to bile by a transcriptomic and proteomic approach. The analysis revealed that exposure to bile induced changes in the abundance of 52 proteins and the transcript levels of 67 genes. The observed changes affected genes and proteins involved in the stress response, fatty acid and cell wall biosynthesis, metabolism of carbohydrates, transport of peptides, coenzyme levels, membrane H + -ATPase, and a number of uncharacterized genes and proteins. These data provide new insights into the mechanisms that enable L. casei BL23 to cope with bile stress.

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Section: Cell Envelope-related Functionsmentioning

confidence: 86%

Proteomic and transcriptomic analysis of the response to bile stress of Lactobacillus casei BL23

Alcántara

Zúñiga

2012

Microbiology

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“…Under acid stress conditions, Lb. casei and S. mutans markedly decreased the synthesis of the phosphoenolpyruvate phosphotransferase system (PEP-PTS) for glucose, which is the primary carbohydrate transport system belonging to the PTS-GFL superfamily (165)(166)(167). Under the same conditions, an acid-resistant mutant of Lb.…”

Section: Metabolism Of Carbon Sources and Energy Productionmentioning

confidence: 99%

“…Under acidic conditions, they reduce the abundance of amino acid transporters, and FAA become limiting. Consequently, many lactobacilli increase the activities of peptidases, such as PepN and PepO (161,165,170,171). The proteolytic system of LAB is also influenced by carbon metabolism (196).…”

Section: Metabolism Of Nitrogenmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

517

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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“…Induction of stress proteins was also observed in L. casei Zhang (Wu et al, 2011;. In contrast, Broadbent et al observed a decrease in the expression of stress response proteins after acid adaptation.…”

Section: Discussionmentioning

confidence: 89%

“…Hamilton and colleagues reported that most of the proteins induced in response to acid shock in L. casei 151 were expressed between 30 and 60 minutes after shock. Since our previous data suggested that TCS12 inactivation mostly affected the cell envelope and changes in cytoplasmic proteins in response to low pH in L. casei had already been characterized (Wu et al, 2011), we decided to analyze in detail proteins associated to the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Two Component Systems of Lactobacillus casei BL23 and their involvement in stress response

Guarinos¹

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Proteomic analysis of responses of a new probiotic bacterium Lactobacillus casei Zhang to low acid stress

Cited by 91 publications

References 38 publications

Proteomic and transcriptomic analysis of the response to bile stress of Lactobacillus casei BL23

Proteomic and transcriptomic analysis of the response to bile stress of Lactobacillus casei BL23

Stress Physiology of Lactic Acid Bacteria

Characterization of Two Component Systems of Lactobacillus casei BL23 and their involvement in stress response

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