Proteomic analysis of pubocervical fascia in women with and without pelvic organ prolapse and urodynamic stress incontinence

Athanasiou, Stavros; Lymberopoulos, Elias; Kanellopoulou, Sofia; Rodolakis, Alexandros; Vlachos, Georgios; Antsaklis, Aris

doi:10.1007/s00192-010-1203-4

Cited by 8 publications

(8 citation statements)

References 28 publications

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“…Since myosin is responsible for muscle contraction, this overexpression of myosin light chains may be a mechanism to counteract the loss of normal urethral function in SUI. This finding is consistent with previous work showing 4.4-fold overexpression of the myosin light chains in the pubococcygeus muscle of women with POP [20].…”

Section: Discussionsupporting

confidence: 95%

Proteomic analysis of urethral protein expression in an estrogen receptor α-deficient murine model of stress urinary incontinence

Chen

Lin

et al. 2015

World J Urol

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Section: Discussionsupporting

confidence: 95%

Proteomic analysis of urethral protein expression in an estrogen receptor α-deficient murine model of stress urinary incontinence

Chen

Lin

et al. 2015

World J Urol

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“…Since myosin is responsible for muscle contraction, this overexpression of myosin heavy chains may be a mechanism to counteract the loss of normal urethral function in SUI. This finding is consistent with previous works showing overexpression myosin genes in the pubococcygeus muscle of women with POP [23] , [24] .…”

Section: Discussionsupporting

confidence: 94%

“…In label-free analysis (as in any other proteomics method), not all proteins can be resolved simultaneously; this is due to differences in their properties (such as hydrophobicity, charge, and solubility properties). The specific protocol used in our study is optimal for the solubility of cytosolic proteins [24] . Changes in the protein extraction protocol are needed for easier detection of ECM proteins.…”

Section: Discussionmentioning

confidence: 99%

Urethral Dysfunction in Female Mice with Estrogen Receptor β Deficiency

Chen

Yeh

et al. 2014

PLoS ONE

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Estrogen has various regulatory functions in the growth, development, and differentiation of the female urogenital system. This study investigated the roles of ERβ in stress urinary incontinence (SUI). Wild-type (ERβ+/+) and knockout (ERβ−/−) female mice were generated (aged 6–8 weeks, n = 6) and urethral function and protein expression were measured. Leak point pressures (LPP) and maximum urethral closure pressure (MUCP) were assessed in mice under urethane anesthesia. After the measurements, the urethras were removed for proteomic analysis using label-free quantitative proteomics by nano-liquid chromatography–mass spectrometry (LC-MS/MS) analysis. The interaction between these proteins was further analysed using MetaCore. Lastly, Western blot was used to confirm the candidate proteins. Compared with the ERβ+/+ group, the LPP and MUCP values of the ERβ−/− group were significantly decreased. Additionally, we identified 85 differentially expressed proteins in the urethra of ERβ−/− female mice; 57 proteins were up-regulated and 28 were down-regulated. The majority of the ERβ knockout-modified proteins were involved in cell-matrix adhesion, metabolism, immune response, signal transduction, nuclear receptor translational regelation, and muscle contraction and development. Western blot confirmed the up-regulation of myosin and collagen in urethra. By contrast, elastin was down-regulated in the ERβ−/− mice. This study is the first study to estimate protein expression changes in urethras from ERβ−/− female mice. These changes could be related to the molecular mechanism of ERβ in SUI.

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“…Papers assessing the whole genome/proteome expression profile in POP did not provide consistent results (Table 1 ). Two studies on pubococcygeus tissue in POP patients have shown both up- and down-regulation of cytoskeletal genes or proteins 10 , 11 . In full-thickness vaginal wall biopsies, genes related to smooth muscle contraction, proteolysis, response to oxidative stress, transcriptional regulation, cytoskeletal organization, and lipid catabolism were over-expressed 12 .…”

Section: Resultsmentioning

confidence: 99%

“… The expression levels of transgelin (FC 54.3), smooth muscle gamma-actin (FC 6.3), myosin light polypeptide 6 (FC 4.4), and alpha-1 antitrypsin precursor (FC 4.3) were higher in patients than in controls. 2-DE western blot analysis for transgelin 11 USLs from Asian women with POP-Q stage 3-4 (cases) or 0-1 (controls) 12 cases (PM), 5 controls (PM) Microarray gene expression 143 genes were up-regulated and 87 genes were down-regulated with FC > 1.5 ( P < 0.05). The expression levels of ESRRA (FC 0.434) decreased, while the expression levels of DAPK2 (FC 2.013), IL15 (FC 2.1) and STAP2 (FC 2.392) increased in POP patients compared with controls.…”

Section: Resultsmentioning

confidence: 99%

Expression changes in pelvic organ prolapse: a systematic review and in silico study

Khadzhieva

Kolobkov

Камоева

et al. 2017

Sci Rep

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Pelvic organ prolapse (POP) is a highly disabling condition common for a vast number of women worldwide. To contribute to existing knowledge in POP pathogenesis, we performed a systematic review of expression studies on both specific gene and whole-genome/proteome levels and an in silico analysis of publicly available datasets related to POP development. The most extensively investigated genes in individual studies were related to extracellular matrix (ECM) organization. Three premenopausal and two postmenopausal sets from two Gene Expression Omnibus (GEO) studies (GSE53868 and GSE12852) were analyzed; Gene Ontology (GO) terms related to tissue repair (locomotion, biological adhesion, immune processes and other) were enriched in all five datasets. Co-expression was higher in cases than in controls in three premenopausal sets. The shared between two or more datasets up-regulated genes were enriched with those related to inflammatory bowel disease (IBD) in the NHGRI GWAS Catalog. ECM-related genes were not over-represented among differently expressed genes. Up-regulation of genes related to tissue renewal probably reflects compensatory mechanisms aimed at repair of damaged tissue. Inefficiency of this process may have different origins including age-related deregulation of gene expression.

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Proteomic analysis of pubocervical fascia in women with and without pelvic organ prolapse and urodynamic stress incontinence

Abstract: This pilot study is the first study to estimate changes in protein expression in the pubocervical fascia of human patients with POP. These changes could be related to the pathophysiology of POP.

Cited by 8 publications

References 28 publications

Proteomic analysis of urethral protein expression in an estrogen receptor α-deficient murine model of stress urinary incontinence

Proteomic analysis of urethral protein expression in an estrogen receptor α-deficient murine model of stress urinary incontinence

Urethral Dysfunction in Female Mice with Estrogen Receptor β Deficiency

Expression changes in pelvic organ prolapse: a systematic review and in silico study

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