Proteomic analysis of protein expression profiles during hyperthermia-induced apoptosis in Tca8113 cells

Abstract

The aim of the present study was to explore protein expression profiles during cancer cell apoptosis induced by hyperthermia. A hyperthermia-induced apoptosis model was established using a Tca8113 cell line derived from a human tongue squamous cell carcinoma, which underwent fluorescent differential display two-dimensional (2D) gel electrophoresis at 2, 6, 8, 12 and 24 h following the induction of hyperthermia. Proteins were identified by mass spectrometry analysis. Expression changes in the proteins were detected by western blot analysis. A total of 107 proteins were detected that exhibited different expression levels in the hyperthermia-treated cells compared with the controls, and 57 of these proteins were identified. Expression changes in the representative proteins were further verified by western blot analysis. These 57 proteins were identified according to the following functional groups: energy metabolism-related enzymes, cytoskeleton-related proteins, chaperones, transcription factors, protein synthesis-related proteins and cell division- and proliferation-related proteins. These groups included 44 upregulated and 13 downregulated proteins. Among the 44 upregulated proteins, 27 were upregulated continuously, eight were upregulated at an early time-point and nine were upregulated at a middle to late time-point. Among the 13 downregulated proteins, five were downregulated continuously, six were downregulated at an early time-point and two were downregulated at a middle to late time-point. These results indicate that hyperthermia-induced Tca8113 cell apoptosis is controlled by multiple factors, which include time and regulatory proteins.

Genetic architecture of acute hyperthermia resistance in juvenile rainbow trout (Oncorhynchus mykiss) and genetic correlations with production traits

“…The phb ( prohibitin ) gene encodes a highly conserved protein with roles in many diverse functions such as chaperoning activities in the mitochondria [ 108 ], the activation of transcription signalling pathways [ 109 ] or the regulation of cell survival and apoptosis [ 110 ]. This gene is upregulated under acute heat stress in the salt marsh mussel Geukensia demissa [ 111 ] and in a cell line derived from a human tongue squamous cell carcinoma [ 112 ]. These two studies, however, disagree on the presumed role of the prohibitin protein: Fields et al [ 111 ] hypothesised that the prohibitin level increased in cells to delay or prevent heat-induced apoptosis, while Jiang et al [ 112 ] argued that the increased abundance of prohibitin in cells may promote their apoptosis.…”

“…This gene is upregulated under acute heat stress in the salt marsh mussel Geukensia demissa [ 111 ] and in a cell line derived from a human tongue squamous cell carcinoma [ 112 ]. These two studies, however, disagree on the presumed role of the prohibitin protein: Fields et al [ 111 ] hypothesised that the prohibitin level increased in cells to delay or prevent heat-induced apoptosis, while Jiang et al [ 112 ] argued that the increased abundance of prohibitin in cells may promote their apoptosis. Since prohibitin has many functions, some of which are poorly understood, it is difficult to hypothesize what effect this protein might have on resistance to acute hyperthermia in rainbow trout.…”

Genetic architecture of acute hyperthermia resistance in juvenile rainbow trout (Oncorhynchus mykiss) and genetic correlations with production traits

“…Prohibitin (phb) is a highly conserved protein involved in many diverse functions such as chaperoning activities in mitochondria [108], the activation of transcription signalling pathways [109] or the regulation of cell survival and apoptosis [110]. phb was shown to be upregulated under acute heat stress in salt marsh mussel Geukensia demissa [111] and in a cell line derived from a human tongue squamous cell carcinoma [112]. These two studies disagree on the presumed role of prohibitin: Fields et al (2016) hypothesised that prohibitin level increased in cells to delay or prevent heat-induced apoptosis while Jiang et al (2013) argued that the increased abundance of prohibitin in cells may promote their apoptosis.…”

“…(2016) hypothesised that prohibitin level increased in cells to delay or prevent heat-induced apoptosis while Jiang et al (2013) argued that the increased abundance of prohibitin in cells may promote their apoptosis. Since prohibitin has many functions, some of which are poorly understood, it is difficult to know what effect this protein might have on resistance to acute hyperthermia in rainbow trout.…”

Nanoparticle-Mediated Hyperthermia and Cytotoxicity Mechanisms in Cancer

“…This results in augmented tumor porosity that facilitates NP redistribution [ 29 ]. Alterations in apoptosis-associated transcription factors, such as heat shock transcription factor 1 (HSF1) [ 30 , 31 ], gene expression modifications [ 32 , 33 , 34 ], and regulation of anti/pro-apoptotic proteins, such as the anti-apoptotic Bcl-2 protein and the p53 tumor suppressor protein [ 35 , 36 ], have been identified as physiological changes in cancer cells. In fact, utilization of HTT is not utopic as heat shock protein expression may arise [ 37 ], thus acting protectively for the tumor by maintaining the growth and survival of cancer cells.…”