Proteomic analysis of porcine pancreas development

Abstract

Porcine pancreas development is not well studied at the molecular level despite being a therapeutic resource for diabetic patients. In this study, we investigated expression of lineage markers and performed proteomic analysis. Expression of the early lineage markers Pdx1 and Ptf1a was developmentally conserved between mice and pigs, whereas expression of the islet differentiation marker Pax4 was delayed in porcine compared with murine pancreas development. Proteomic analysis found that expression levels of chymotrypsinogen were downregulated during porcine pancreas development while those of digestive enzymes like lipases, elastase and serine protease were up-regulated. In addition, specific isoforms of protein folding assistants such as protein disulfide isomerase and prefoldin were expressed at specific stages during the maturation of digestive enzymes. Taken together, these results show that development of the porcine pancreas is regulated by a concerted interplay of pancreas lineage marker proteins and other specified proteins, resulting in a functional endocrine and exocrine organ. [BMB reports 2009; 42(10): 661-666]

A Genome-Wide Association Study on Feed Efficiency Related Traits in Landrace Pigs

“… Do et al (2014b) reported that insulin signaling pathway plays important roles in controlling RFI in Duroc pigs, and it has been shown that insulin also affects feed intake and feed behavior in chickens ( Shiraishi et al, 2008 , 2011 ). Moreover, many reports showed that PDX1 was closely related to porcine pancreas development ( Choi et al, 2009 ) and diabetes ( Matsunari et al, 2014 ). Pancreas and diabetes are closely related to feed intake, digestion, absorption, and metabolism.…”

Proteome analysis of Thermococcus onnurineus NA1 reveals the expression of hydrogen gene cluster under carboxydotrophic growth

“…Clusters of orthologous groups of proteins were categorized and constructed using the NCBI genome database [3]. Relative protein abundance based on the emPAI from CO-metabolizing hydrogenase clusters was performed using Excel 2007 and DAnTE v1.0, both R-based bioinformatics tools, as described previously [18].…”

A comprehensive analysis of prefoldins and their implication in cancer

“…In porcine pancreas, PFDN presents a differential expression according to the stage of the development, being downregulated after birth, following similar expression patterns as other cytoskeletal regulatory proteins ( Choi et al., 2009 ). Surprisingly, auto-antibodies against PFDN2 were found significantly enriched in type 2 diabetes patients, especially in those with an early onset of the disease ( Chang et al, 2017 ).…”