Proteomic Analysis of Peripheral T-Lymphocytes in Patients With Asthma

Jeong, Hoyoung; Lee, Sang Yeub; Jung, Ki Hwan; Kang, Eun Hae; Lee, Sung Yong; Kim, Je Hyeong; Park, Eun Kyung; Lee, Sang Hoon; Uhm, Chang Sub; Cho, Yunjung; Shin, Chol; Shim, Jae Jeong; Kim, Han Kyeom; In, Kwang Ho; Kang, Kyung Ho; Yoo, Se Hwa

doi:10.1378/chest.06-2980

Cited by 28 publications

(19 citation statements)

References 49 publications

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“…Widely used as part of these endeavors are proteomics-and genomics-based approaches, which have indeed identified novel inflammatory biomarkers in human and experimental asthma [5][6][7]. Bronchoalveolar lavage (BAL) fluid is perhaps the most widely used biological sample for the identification of novel asthma biomarkers through proteomics, although serum, whole lung and CD3 + T cells have also been used [8][9][10][11]. Allergic inflammation affects essentially all structures within the lung, but the airway epithelium is perhaps most strikingly altered.…”

mentioning

confidence: 99%

Discovery of novel markers in allergic lung inflammation through proteomic-based technologies

Kheradmand¹,

Corry²

2008

Expert Review of Proteomics

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mentioning

confidence: 99%

Discovery of novel markers in allergic lung inflammation through proteomic-based technologies

Kheradmand¹,

Corry²

2008

Expert Review of Proteomics

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“…Despite these limitations, the coupling of the iTRAQ ® technology and the nanoLC-LTQ-Orbitrap XL instrument successfully identified a large number of proteins in the samples, including many that were relevant to asthma and respiratory diseases. As might be expected from tissue samples, the currently applied technique was able to identify and quantify a larger number of proteins than any previous proteomic study in asthma using BALF [10] or specific cells [11,29], despite no affinity depletion of high abundance proteins. Interestingly, the low abundance of cytokines usually prevents their quantification in proteomics studies.…”

Section: Discussionmentioning

confidence: 97%

“…The majority of studies concerning asthma have been conducted on bronchoalveolar lavage fluid (BALF) and lung tissue of mouse models [4-7], which can only represent certain features of asthma. The few efforts in human asthma have mainly centred around cell cultures [8], sputum [9], BALF [10], T lymphocytes [11] and more recently, exhaled breath condensate [12]. While these samples present aspects of asthma, they do not totally reflect the site of disease, the bronchi.…”

Section: Introductionmentioning

confidence: 99%

Network analysis of quantitative proteomics on asthmatic bronchi: effects of inhaled glucocorticoid treatment

et al. 2011

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BackgroundProteomic studies of respiratory disorders have the potential to identify protein biomarkers for diagnosis and disease monitoring. Utilisation of sensitive quantitative proteomic methods creates opportunities to determine individual patient proteomes. The aim of the current study was to determine if quantitative proteomics of bronchial biopsies from asthmatics can distinguish relevant biological functions and whether inhaled glucocorticoid treatment affects these functions.MethodsEndobronchial biopsies were taken from untreated asthmatic patients (n = 12) and healthy controls (n = 3). Asthmatic patients were randomised to double blind treatment with either placebo or budesonide (800 μg daily for 3 months) and new biopsies were obtained. Proteins extracted from the biopsies were digested and analysed using isobaric tags for relative and absolute quantitation combined with a nanoLC-LTQ Orbitrap mass spectrometer. Spectra obtained were used to identify and quantify proteins. Pathways analysis was performed using Ingenuity Pathway Analysis to identify significant biological pathways in asthma and determine how the expression of these pathways was changed by treatment.ResultsMore than 1800 proteins were identified and quantified in the bronchial biopsies of subjects. The pathway analysis revealed acute phase response signalling, cell-to-cell signalling and tissue development associations with proteins expressed in asthmatics compared to controls. The functions and pathways associated with placebo and budesonide treatment showed distinct differences, including the decreased association with acute phase proteins as a result of budesonide treatment compared to placebo.ConclusionsProteomic analysis of bronchial biopsy material can be used to identify and quantify proteins using highly sensitive technologies, without the need for pooling of samples from several patients. Distinct pathophysiological features of asthma can be identified using this approach and the expression of these features is changed by inhaled glucocorticoid treatment. Quantitative proteomics may be applied to identify mechanisms of disease that may assist in the accurate and timely diagnosis of asthma.Trial registrationClinicalTrials.gov registration NCT01378039

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“…CD4 + T lymphocytes, especially Th2 lymphocytes, play an important role in the initiation, progression and persistence of asthma [48]. The expression of 13 proteins increased and 12 decreased in T lymphocytes from asthmatic patients, as compared to the healthy [49]. Among them, phosphodiesterase 4C, heat shock protein (HSP)-70, protein tyrosine phosphatase, β-arrestin-1b, thioredoxin-like 2, LLR protein, glutathione reductase, major histocompatibility complex class I antigen, epidermal growth factor receptor, pyrroline 5-carboxylate reductase isoform, and FLJ25770 protein increased, while dynein, cytoplasmic light intermediate polypeptide 2, vimentin, zinc finger protein 76, tubulin β 2 , T-cell receptor β-chain, cyclin-dependent kinase 6, pyridoxal kinase, phenylalanine hydroxylase, glutathione S-transferase-M3, and tetratricopeptide repeat-containing protein decreased.…”

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of lymphocytes in autoimmunity, inflammation and cancer

et al. 2014

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Lymphocytes play important roles in the balance between body defense and noxious agents involved in a number of diseases, e.g. autoimmune diseases, allergic inflammation and cancer. The proteomic analyses have been applied to identify and validate disease-associated and disease-specific biomarkers for therapeutic strategies of diseases. The proteomic profiles of lymphocytes may provide more information to understand their functions and roles in the development of diseases, although proteomic approaches in lymphocytes are still limited. The present review overviewed the proteomics-based studies on lymphocytes to headlight the proteomic profiles of lymphocytes in diseases, such as autoimmune diseases, allergic inflammation and cancer, with a special focus on lung diseases. We will explore the potential significance of diagnostic biomarkers and therapeutic targets from the current status in proteomic studies of lymphocytes and discuss the value of the currently available proteomic methodologies in the lymphocytes research.

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Proteomic Analysis of Peripheral T-Lymphocytes in Patients With Asthma

Cited by 28 publications

References 49 publications

Discovery of novel markers in allergic lung inflammation through proteomic-based technologies

Discovery of novel markers in allergic lung inflammation through proteomic-based technologies

Network analysis of quantitative proteomics on asthmatic bronchi: effects of inhaled glucocorticoid treatment

Proteomic profiling of lymphocytes in autoimmunity, inflammation and cancer

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