Proteomic analysis of osteoarthritic chondrocyte reveals the hyaluronic acid-regulated proteins involved in chondroprotective effect under oxidative stress

Yu, Chia Jung; Ko, Chun-Jung; Hsieh, Chang‐Hsun; Chien, Chiang Ting; Huang, Lien‐Hung; Lee, Chien‐Wei; Jiang, Ching-Chuan

doi:10.1016/j.jprot.2014.01.016

Cited by 53 publications

(48 citation statements)

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“…Oxidative stress is regarded as a major cause of the degradation of chondrocytes and articular cartilage, which results in the pathogenesis of OA and the aging of cartilage (9,10,16). Several previous reports have focussed on the various strategies used to resist oxidative stress (14,28).…”

Section: Discussionmentioning

confidence: 99%

“…Reactive oxygen species (ROS), another major determinant of stress, including hydrogen peroxide (H 2 O 2 ), hypochlorite ion, hydroxyl radical and superoxide anion, are involved in normal intracellular transduction and degenerative cellular processes. Increased ROS production is considered to be an important cause of chondrocyte dysfunction and articular cartilage degradation, which leads to the pathogenesis of OA and the aging of cartilage (7)(8)(9)(10). Chondrocytes and cartilage tissues exhibit efficient protective strategies to minimize damage induced by increased ROS production, including H 2 O 2 (7,11).…”

Section: Introductionmentioning

confidence: 99%

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Ascorbic acid provides protection for human chondrocytes against oxidative stress

Chang

Huo

et al. 2015

Molecular Medicine Reports

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Abstract. Oxidative stress is considered to be an important cause of dysfunction in chondrocytes and articular cartilage degradation, which leads to the pathogenesis of osteoarthritis (OA) and cartilage aging. The present study aimed to assess the effects of the widely applied antioxidant, ascorbic acid (AA), on human chondrocytes against hydrogen peroxide (H 2 O 2 ) in vitro. Using annexin V-fluorescein isothiocyanate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and senescence-associated β-galactosidase assays, the present study identified that AA reduced apoptosis, reduced the loss of viability and markedly decreased H 2 O 2 -mediated senescence in cells treated with H 2 O 2 . Furthermore, AA not only stimulated the expression levels of collagens and proteoglycans, but also inhibited the differentiation of chondrocytes under conditions of oxidative stress. In addition, reverse transcription-quantitative polymerase chain reaction and western blotting demonstrated that AA decreased the activity of nrf2, NF-κB, AP1 and matrix metalloproteinase-3, which is stimulated by H 2 O 2 . In conclusion, AA efficiently protected human chondrocytes against damage induced by H 2 O 2 by regulating multiple regulatory pathways.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Ascorbic acid provides protection for human chondrocytes against oxidative stress

Chang

Huo

et al. 2015

Molecular Medicine Reports

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“…We also observed in our laboratory that the addition of HA can mitigate the activity of reactive oxygen species (ROS). ROS can cause death of chondrocytes when present in concentrations above 0.9 mM [25]. The palliation by HA is attributable to the redox capability of the molecule, which can give up and/or grab two electrons per base unit [26].…”

Section: Introductionmentioning

confidence: 99%

Phospholipid Vesicles in Media for Tribological Studies against Live Cartilage

et al. 2018

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Introduction Pre-clinical testing of hemiarthroplasty devices requires that the tribological conditions present in vivo with live cartilage be closely duplicated. A current limitation in the tribological testing of live cartilage involves the use of cell-culture media as lubricant. Study Aim to develop and test a new hyaluronan-phospholipid based medium (HA–phospholipid medium) that combines the rheological and frictional properties of synovial fluid with the nourishing properties of culture media to keep cells alive. Materials and Methods The HA–phospholipid medium consisted of culture medium with added phospholipid dipalmitoylphosphatidylcholine (0.3 mg/mL), and hyaluronic acid (2.42 mg/mL). A standard cell culture medium was used as the control. The rheology of each medium was determined using a flat plate configuration. Bovine calf cartilage was used to assess cell viability and friction in each medium. For friction measurements, a cobalt-chrome alloy ball was articulated against cartilage disks immersed in medium. Results Lipid vesicles 0.1 to 50 μm in diameter were identified in the HA–phospholipid medium. Cartilage cell viability was significantly higher in the HA–phospholipid medium (62% ± 8%, 95% CI) than in control medium (49.5% ± 5%) (p = 0.009). The HA–phospholipid medium exhibited strong shear-thinning behavior, similar to synovial fluid, with viscosities ~100-fold higher at 10 s−1 and 5-fold higher at 20,000 s−1 than the approximately Newtonian control medium. The HA–phospholipid medium also yielded 20% lower friction values than the control medium after one hour of testing. Conclusions The rheological and friction results indicate that the HA–phospholipid medium is superior to the control cell culture medium in emulating the shear thinning and lubricative properties of natural synovial fluid, making it more clinically relevant for in vitro wear and friction testing with live cartilage.

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“…Each of these enzymes were isolated from the rat heart during the experiments proposed by Western Blot technique. [32][33][34][35] Regarding the Western Blot analysis, the myocardial tissue must be taken and frozen quickly enough to avoid kinase degradation under the action of the existing proteases from the tissue. To achieve this goal, the heart was excised just before stopping in asystole, rinsed quickly with cold saline solution at 4°C, then suddenly introduced in liquid nitrogen (-196°C).…”

Section: Western-blot Technique For Determining the Kinases Of The Camentioning

confidence: 99%

Cardioprotective Effects Induced by Preconditioning with Halogenated Anesthetics

Păpurică¹,

Săndesc²,

Rogobete³

et al. 2016

Journal of Interdisciplinary Medicine

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backgroundIn the organ transplantation field, a very intense and important topic is to find ways to increase the quality of organs that are taken from willing donors, in order to improve the recovery capacity of the transplant recipients. 1 From the moment of the organ removal until its actual transplantation, there is a variable period of ischemia, named cold-ischemia, responsible for the production Cardioprotective Effects Induced by Preconditioning with Halogenated AnestheticsMarius Papurica 1,2 , Dorel Sandesc 1,2 , Alexandru Florin Rogobete 1,2 , Radu Nartita 3 , Corina Vernic 2 , Sonia Elena Popovici 2 , Ovidiu Horea Bedreag 1,2 1 Clinic of Anesthesia and Intensive Care, "Pius Brinzeu" Emergency County Hospital, Timișoara, Romania 2 Faculty of Medicine, "Victor Babeș" University of Medicine and Pharmacy, Timișoara, Romania 3 Faculty of Chemistry, Biology, Geography, West University of Timișoara, Romania ABSTRACT Background: Numerous studies discuss the protective effects of halogenated anesthetics on myocyte injury induced by the ischemia-reperfusion syndrome of the heart. This mechanism is known as pharmacological preconditioning. Aim of the study: The objective of this study was to identify the effects of two volatile anesthetics frequently used in current clinical practice, Isoflurane and Sevoflurane, on the in situ heart. The study was performed on laboratory animals with induced brain death. Material and methods: The animals were divided into 3 groups, the control group (n = 8), IZO-PRE group (n = 8) and SEVO-PRE group (n = 8), on which the experimental protocol established for this study was applied. From a molecular point of view, the expressions of protein kinase C-epsilon (PKCε) and of glycogen synthase kinase -3 beta (GSK-3β) were investigated. Results: Following the statistical analysis, we observed a significant reduction in the size of the infarcted area in the IZO-PRE group compared to the control group (p <0.0001). Regarding the SEVO-PRE group, no reduction was observed (p >0.05). The expression of GSK-3β is more pronounced in case of the SEVO-PRE group, at 5 minutes after reperfusion, and the effect disappears after 15 minutes. The expression of PKCε as a total form of the enzyme, occurs at 5 minutes after reperfusion in the SEVO-PRE group and late in the IZO-PRE group (after 15 minutes). Conclusions: Both anesthetics that were applied showed a cardioprotective effect. Isoflurane provided a better structural and morphological protection, but Sevoflurane resulted in a more effective protection in terms of functionality, significantly reducing the incidence of extremely severe life-threatening arrhythmias.

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Proteomic analysis of osteoarthritic chondrocyte reveals the hyaluronic acid-regulated proteins involved in chondroprotective effect under oxidative stress

Cited by 53 publications

References 48 publications

Ascorbic acid provides protection for human chondrocytes against oxidative stress

Ascorbic acid provides protection for human chondrocytes against oxidative stress

Phospholipid Vesicles in Media for Tribological Studies against Live Cartilage

Cardioprotective Effects Induced by Preconditioning with Halogenated Anesthetics

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