Proteomic analysis of mycelial proteins from Magnaporthe oryzae under nitrogen starvation

Abstract

ABSTRACT.Magnaporthe oryzae is an important model system in studies of plant pathogenic fungi, and nitrogen is a key nutrient source affecting microbial growth and development. In order to understand how nitrogen stress causes changes in mycelial proteins, we analyzed differentially expressed mycelial proteins from the M. oryzae virulent strain CH-63 using two-dimensional electrophoresis and mass spectrometry in complete medium or under nitrogen starvation conditions. A total of 975 ± 70 and 1169 ± 90 protein spots were detected in complete medium and under nitrogen starvation conditions, respectively. Forty-nine protein spots exhibited at least 2-fold upregulation or down-regulation at the protein level according to PDQuest7.4. Moreover, 43 protein spots were successfully identified by matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight mass spectrometry. Among these spots, 6 proteins were functionally unknown and 37 proteins were categorized into 5 groups according to their functions, including development, metabolism, biosynthesis, and biological process. These 37 proteins were further analyzed for their enriched metabolic pathways by KOBAS2.0, and 14 proteins were found to be involved in glycolysis, tricarboxylic acid cycle, and nitrogen metabolism. Taken together, the regulation of M. oryzae growth under the nitrogen starvation conditions appears to be complex because of the various proteins and enzymes involved.

Genome-wide association mapping for growth rate at fluctuating and extreme temperatures

“…6). The first association was upstream from the gene (NCU03028) encoding a deubiquitination-protection protein dph1, which works in protein degradation and localization to vacuoles, and has been found to affect mycelial growth under starvation stress (Zhou et al 2016). The second association was in the gene (NCU06948) encoding an EF-hand domain-containing protein and had a very strong allelic effect (– 0.99 mm/h).…”

Extraction and characterisation of proteins from a Malaysian Isolate of Magnaporthe grisea

“…tritici (Broeker et al, 2006;Ling et al, 2007). In addition, TALs play a key role in a variety of life activities, development, and metabolism (Zhou et al, 2016). The fructose 1.6 biphosphate aldolase (FBA) class II enzyme is critical for glycolysis or gluconeogenesis (Capodagli et al, 2013) in M. oryzae (Mandelc et al, 2009).…”

“…Here we have subjected our own field isolate to protein isolation and characterisation for the identification of proteins that may play a critical role in fungal pathogenicity and virulence. However, among all the proteins isolated, only a few were known and characterised to be involved in appressorium formation through annotation against the published genome sequence of M. grisea (Kim et al, 2004;Zhou et al, 2016).…”