Proteomic analysis of multidrug resistant Escherichia coli strains from scouring calves

Gautam, Ablesh; Vinson, Heather M.; Gibbs, Penelope S.; Olet, Susan; Barigye, Robert

doi:10.1016/j.vetmic.2011.03.032

Cited by 5 publications

(7 citation statements)

References 34 publications

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“…Up to now, E. coli quantitative proteomics have been used either for comparison between strains in a given environment (Gautam et al ., ; Smith et al ., ), or for assessing the bases of phenotypic plasticity during environmental change (Lopez‐Campistrous et al ., ; Kocharunchitt et al ., ). Furthermore, E. coli quantitative proteomics have been mainly focused on the laboratory derived K‐12 strain MG1655, using its mutants in a given environment and/or during environmental changes (Vollmer et al ., ; Lopez‐Campistrous et al ., ).…”

Section: Discussionmentioning

confidence: 97%

Interactions between genotype and environment drive the metabolic phenotype within Escherichia coli isolates

Sabarly

Aubron

Glodt

et al. 2015

Environmental Microbiology

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To gain insights into the adaptation of the Escherichia coli species to different environments, we monitored protein abundances using quantitative proteomics and measurements of enzymatic activities of central metabolism in a set of five representative strains grown in four contrasted culture media including human urine. Two hundred and thirty seven proteins representative of the genome-scale metabolic network were identified and classified into pathway categories. We found that nutrient resources shape the general orientation of metabolism through coordinated changes in the average abundances of proteins and in enzymatic activities that all belong to the same pathway category. For example, each culture medium induces a specific oxidative response whatever the strain. On the contrary, differences between strains concern isolated proteins and enzymes within pathway categories in single environments. Our study confirms the predominance of genotype by environment interactions at the proteomic and enzyme activity levels. The buffering of genetic variation when considering life-history traits suggests a multiplicity of evolutionary strategies. For instance, the uropathogenic isolate CFT073 shows a deregulation of iron demand and increased oxidative stress response.

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Section: Discussionmentioning

confidence: 97%

Interactions between genotype and environment drive the metabolic phenotype within Escherichia coli isolates

Sabarly

Aubron

Glodt

et al. 2015

Environmental Microbiology

View full text Add to dashboard Cite

show abstract

“…Gautam et al . () reported a difference in the number of peptides recovered from gels of fractionated antimicrobial susceptible and MDR protein extracts suggesting that OmpA may play a role in drug resistance. In our study, the upregulation of OmpA upon exposure to AMP in the WT strain indicated that it may directly respond to AMP by initiating an efflux mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…A study done by Wang (2002) also showed that OmpA-null E. coli K-1 mutants were sensitive to SDS, cholate, acidic environment, high osmolarity and serum when compared with the WT strains. Gautam et al (2011) reported a difference in the number of peptides recovered from gels of fractionated antimicrobial susceptible and MDR protein extracts suggesting that OmpA may play a role in drug resistance. In our study, the upregulation of OmpA upon exposure to AMP in the WT strain indicated that it may directly respond to AMP by initiating an efflux mechanism.…”

Section: Discussionmentioning

confidence: 99%

“…Gautam et al . () showed that the variable expression patterns of proteins obtained from multidrug resistant and antimicrobial susceptible Escherichia coli strains were grouped into membrane porins (TolC, OmpA, OmpC and NmpC precursor), microbial protein synthesis‐associated proteins (RspA, EF‐Ts and EF‐Ts), plus Dps which might play a role associated with drug resistance. Yu and Guo () investigated and compared proteome‐wide alterations of Salm.…”

Section: Introductionmentioning

confidence: 99%

“…These proteins are related to resistance mechanisms, modification of xenobiotics or drug transport, stress response, porins, outer membrane proteins, transporters and secretion, cell wall-related proteins, lipoproteins and DNA-or plasmid-related proteins. Gautam et al (2011) showed that the variable expression patterns of proteins obtained from multidrug resistant and antimicrobial susceptible Escherichia coli strains were grouped into membrane porins (TolC, OmpA, OmpC and NmpC precursor), microbial protein synthesis-associated proteins (RspA, EF-Ts and EF-Ts), plus Dps which might play a role associated with drug resistance. Yu and Guo (2011) investigated and compared proteome-wide alterations of Salm.…”

Section: Introductionmentioning

confidence: 99%

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