Proteomic analysis of mouse ovaries during the prepubertal stages

Xiong, Jiaqiang; Wu, Meng; Zhang, Qinghua; Zhang, Chun; Xiong, Guoping; Ma, Lingwei; Lu, Zhongxin; Wang, Shixuan

doi:10.1016/j.yexcr.2019.02.016

Cited by 15 publications

(7 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…KEGG functional annotation of DE mRNAs related to female reproduction discovered that ECM‐receptor interaction, Focal adhesion and PI3K‐Akt signalling pathway terms were significantly enriched. Studies showed ECM‐receptor interaction was involved in prepubertal follicular development and ovulation for mice and goats (Wang et al, 2016; Xiong et al, 2019). Focal adhesion was involved in goat ovulation and fecundity (Ling et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

An integrated analysis of mRNAs and lncRNAs in goat's hypothalamus to explore the onset of puberty

Gao

Ren

Zhang

et al. 2023

Reprod Domestic Animals

View full text Add to dashboard Cite

The time of puberty onset is crucial for female animal, as it can affect the generation interval, feeding costs and the utilization of animals. However, little is known about the mechanism of hypothalamic lncRNAs (long noncoding RNAs) in regulatory goat puberty onset. Therefore, genome‐wide transcriptome analysis was performed in goats to clarify the roles of hypothalamic lncRNAs and mRNAs in the onset of puberty. In the present study, the co‐expression network of differentially expressed (DE) mRNAs in goat hypothalamus identified FN1 as the hub gene, and ECM‐receptor interaction, Focal adhesion and PI3K‐Akt signalling pathways are involved in puberty. We also observed the crucial hub transcription factors (TCF12, STAT1, STAT2, GATA3 and TEAD4) associated with reproduction and puberty. Then, genetic correlation analysis of DE mRNAs and DE lncRNAs identified the key lncRNAs involved in puberty. This research supplies a resource for transcriptome studies in goat puberty and indicated DE lncRNAs in the ECM‐receptor interaction pathway were novel candidate regulators for genetic studies on female reproduction.

show abstract

Section: Discussionmentioning

confidence: 99%

An integrated analysis of mRNAs and lncRNAs in goat's hypothalamus to explore the onset of puberty

Gao

Ren

Zhang

et al. 2023

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Padi6 encodes an enzyme of the peptidyl arginine deiminase family and is uniquely expressed in male and female germ cells. The absence of PADI6 protein results in the dispersal of cytoskeletal sheets in oocytes, which ultimately leads to female infertility ( Xiong et al, 2019 ; Bebbere et al, 2020 ). Even though Stk31 , Tbpl2 , and Vrtn are poorly studied in follicle development at the current stage, emerging evidence suggests that these genes are specifically expressed in oocytes and play a crucial role during folliculogenesis ( Olesen et al, 2007 ; Di Pietro et al, 2008 ; Duan et al, 2018 ; Yu et al, 2020 ; Yang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Identification of Biomarkers for Predicting Ovarian Reserve of Primordial Follicle via Transcriptomic Analysis

Liu

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Ovarian reserve (OR) is mainly determined by the number of primordial follicles in the ovary and continuously depleted until ovarian senescence. With the development of assisted reproductive technology such as ovarian tissue cryopreservation and autotransplantation, growing demand has arisen for objective assessment of OR at the histological level. However, no specific biomarkers of OR can be used effectively in clinic nowadays. Herein, bulk RNA-seq datasets of the murine ovary with the biological ovarian age (BOA) dynamic changes and single-cell RNA-seq datasets of follicles at different stages of folliculogenesis were obtained from the GEO database to identify gene signature correlated to the primordial follicle pool. The correlations between gene signature expression and OR were also validated in several comparative OR models. The results showed that genes including Lhx8, Nobox, Sohlh1, Tbpl2, Stk31, and Padi6 were highly correlated to the OR of the primordial follicle pool, suggesting that these genes might be used as biomarkers for predicting OR at the histological level.

show abstract

“…p27 -/mice also exhibit massive PFA that is associated with premature ovarian failure in adulthood (22), while deletions of Pdk1 and rpS6 have been shown to trigger follicular loss via accelerated atresia (23). Recently, analysis of human oocyte transcriptomic profiles revealed an enrichment of both PI3K/AKT and mTOR signaling pathways during the primordial-to-primary follicle transition (24,25), a finding that Downloaded from https://academic.oup.com/edrv/advance-article/doi/10.1210/endrev/bnaa020/5882019 by Universite Libre de Bruxelles user on 17 August 2020 A c c e p t e d M a n u s c r i p t 10 was later validated by proteomic analysis using mouse ovaries at different prepubertal stages (26). The expression of PTEN in GCs from human PFs has been confirmed at the protein and gene expression levels (25,27), and AKT is expressed in both oocytes and GCs of human follicles (27,28).…”

Section: A Signaling Network That Regulate Primordial Follicle Actimentioning

confidence: 96%

Implications of Nonphysiological Ovarian Primordial Follicle Activation for Fertility Preservation

2020

View full text Add to dashboard Cite

In recent years, ovarian tissue cryopreservation has rapidly developed as a successful method for preserving the fertility of girls and young women with cancer or benign conditions requiring gonadotoxic therapy, and is now becoming widely recognized as an effective alternative to oocyte and embryo freezing when not feasible. Primordial follicles are the most abundant population of follicles in the ovary, and their relatively quiescent metabolism makes them more resistant to cryoinjury. This dormant pool represents a key target for fertility preservation strategies as a resource for generating high quality oocytes. However, development of mature, competent oocytes derived from primordial follicles is challenging, particularly in larger mammals. One of the main barriers is the substantial knowledge gap regarding the regulation of the balance between dormancy and activation of primordial follicles to initiate their growing phase. In addition, experimental and clinical factors also impact dormant follicle demise, while the mechanisms involved remain largely to be elucidated. Moreover, most of our basic knowledge of these processes comes from rodent studies and should be extrapolated to humans with caution, considering the differences between species in the reproductive field. Overcoming these obstacles is essential to improving both the quantity and the quality of mature oocytes available for further fertilization, and may have valuable biological and clinical applications, especially in fertility preservation procedures. This review provides an update on current knowledge of mammalian primordial follicle activation under both physiological and non-physiological conditions, and discusses implications for fertility preservation and priorities for future research.

show abstract

Proteomic analysis of mouse ovaries during the prepubertal stages

Cited by 15 publications

References 57 publications

An integrated analysis of mRNAs and lncRNAs in goat's hypothalamus to explore the onset of puberty

An integrated analysis of mRNAs and lncRNAs in goat's hypothalamus to explore the onset of puberty

Identification of Biomarkers for Predicting Ovarian Reserve of Primordial Follicle via Transcriptomic Analysis

Implications of Nonphysiological Ovarian Primordial Follicle Activation for Fertility Preservation

Contact Info

Product

Resources

About