Proteomic analysis of MCF-7 cells treated with benzo[a]pyrene, dibenzo[a,l]pyrene, coal tar extract, and diesel exhaust extract

Hooven, Louisa A.; Baird, William M.

doi:10.1016/j.tox.2008.03.019

Cited by 34 publications

(30 citation statements)

References 76 publications

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“…In addition, an increased expression of peptidyl-prolyl cis-trans isomerase B precursor genes (with ID 37367 and 33153) was observed in our study, which is consistent with the up-regulation observed for this protein in MCF-7 cells treated with BaP, where also a HSP70 was among the proteins showing the highest fold increase [33].…”

Section: Discussionsupporting

confidence: 91%

Transcriptomics Responses in Marine Diatom Thalassiosira pseudonana Exposed to the Polycyclic Aromatic Hydrocarbon Benzo[a]pyrene

2011

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Diatoms are unicellular, photosynthetic, eukaryotic algae with a ubiquitous distribution in water environments and they play an important role in the carbon cycle. Molecular or morphological changes in these species under ecological stress conditions are expected to serve as early indicators of toxicity and can point to a global impact on the entire ecosystem. Thalassiosira pseudonana, a marine diatom and the first with a fully sequenced genome has been selected as an aquatic model organism for ecotoxicological studies using molecular tools. A customized DNA microarray containing probes for the available gene sequences has been developed and tested to analyze the effects of a common pollutant, benzo(a)pyrene (BaP), at a sub-lethal concentration. This approach in diatoms has helped to elucidate pathway/metabolic processes involved in the mode of action of this pollutant, including lipid metabolism, silicon metabolism and stress response. A dose-response of BaP on diatoms has been made and the effect of this compound on the expression of selected genes was assessed by quantitative real time-PCR. Up-regulation of the long-chain acyl-CoA synthetase and the anti-apoptotic transmembrane Bax inhibitor, as well as down-regulation of silicon transporter 1 and a heat shock factor was confirmed at lower concentrations of BaP, but not the heat-shock protein 20. The study has allowed the identification of molecular biomarkers to BaP to be later on integrated into environmental monitoring for water quality assessment.

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Section: Discussionsupporting

confidence: 91%

Transcriptomics Responses in Marine Diatom Thalassiosira pseudonana Exposed to the Polycyclic Aromatic Hydrocarbon Benzo[a]pyrene

2011

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“…For example, the carcinogenic polycyclic aromatic hydrocarbon DNA adducts caused multiple alterations in gene expression. A proteomic analysis revealed altered expression of several MRC proteins of MCF-7 cells treated with benzo[a]pyrene [263]. Third, persistent enhanced generation of MRC-derived ROS induced by E2/ERs may cause progressive oxidative damage to mtDNA and mitochondrial proteins, leading to mutations in mtDNA and malfunction of MRC.…”

Section: Physiological and Pathological Implicationsmentioning

confidence: 99%

Regulation of mitochondrial respiratory chain biogenesis by estrogens/estrogen receptors and physiological, pathological and pharmacological implications

Chen

Cammarata

Baines

et al. 2009

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

227

222

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There has been increasing evidence pointing to the mitochondrial respiratory chain (MRC) as a novel and important target for the actions of 17β-estradiol(E2) and estrogen receptors (ER) in a number of cell types and tissues that have high demands for mitochondrial energy metabolism. This novel E2-mediated mitochondrial pathway involves the cooperation of both nuclear and mitochondrial ERα and ERβ and their co-activators on the coordinate regulation of both nuclear DNA- and mitochondrial DNA-encoded genes for MRC proteins. In this paper, we have: 1) comprehensively reviewed studies that reveal a novel role of estrogens and ERs in the regulation of MRC biogenesis; 2) discussed their physiological, pathological and pharmacological implications in the control of cell proliferation and apoptosis in relation to estrogen-mediated carcinogenesis, anticancer drug resistance in human breast cancer cells, neuro-protection for Alzheimer’s disease and Parkinson’s disease in brain, cardiovascular protection in human heart and their beneficial effects in lens physiology related to cataract in the eye; and 3) pointed out new research directions to address the key questions in this important and newly emerging area. We also suggest a novel conceptual approach that will contribute to innovative regimines for the prevention or treatment of a wide variety of medical complications based on E2/ER-mediated MRC biogenesis pathway.

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“…MCF-7 cells treated with benzo[a]pyrene, dibenzo[a,l]pyrene, and coal tar extract showed an increased expression of HSP 70 and HSP 27 [121]. Transformation of nontumorigenic human prostatic epithelial cell line RWPE-1 by in vitro cadmium exposure led to the identification of marked increases in secretion of active MMP-2 and MMP-9, which are secreted enzymes that selectively degrade the extracellular matrix and have been implicated in tumor cell invasion [186].…”

Section: Proteomicsmentioning

confidence: 99%

“…Signal transduction proteins (Nucleoside diphosphate kinase-A and Galectin-3), cytoskeletal proteins (actin cytoplasmic 1, tubulin alpha-1C chain, Myosin light chain alkali, cyclophilin B), DNA-associated proteins (Dihyrodrofolate reductase, Heterogeneous nuclear ribonucleoprotein, histone H2A type 1), and glycolytic and mitochondrial proteins (Alpha enolase and glyceraldehyde-3-phosfate dehydrogenase, ATP synthase) were increased in MCF-7 cells originating from human mammary carcinoma tissue and exposed to benzo[a]pyrene [121]. Finally, an increase of COX-2 expression was found in human sinonasal cancer [122].…”

Section: Proteomicsmentioning

confidence: 99%

Molecular Fingerprints of Environmental Carcinogens in Human Cancer

Ceccaroli

Pulliero

Geretto

et al. 2015

Journal of Environmental Science and Health, Part C

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Identification of specific molecular changes (fingerprints) is important to identify cancer etiology. Exploitable biomarkers are related to DNA, epigenetics, and proteins. DNA adducts are the turning point between environmental exposures and biological damage. DNA mutational fingerprints are induced by carcinogens in tumor suppressor and oncogenes. In an epigenetic domain, methylation changes occurs in specific genes for arsenic, benzene, chromium, and cigarette smoke. Alteration of specific microRNA has been reported for environmental carcinogens. Benzo(a)pyrene, cadmium, coal, and wood dust hits specific heat-shock proteins and metalloproteases. The multiple analysis of these biomarkers provides information on the carcinogenic mechanisms activated by exposure to environmental carcinogens.

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Proteomic analysis of MCF-7 cells treated with benzo[a]pyrene, dibenzo[a,l]pyrene, coal tar extract, and diesel exhaust extract

Cited by 34 publications

References 76 publications

Transcriptomics Responses in Marine Diatom Thalassiosira pseudonana Exposed to the Polycyclic Aromatic Hydrocarbon Benzo[a]pyrene

Transcriptomics Responses in Marine Diatom Thalassiosira pseudonana Exposed to the Polycyclic Aromatic Hydrocarbon Benzo[a]pyrene

Regulation of mitochondrial respiratory chain biogenesis by estrogens/estrogen receptors and physiological, pathological and pharmacological implications

Molecular Fingerprints of Environmental Carcinogens in Human Cancer

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