Proteomic Analysis of Mature Lagenaria siceraria Seed

Kumari, Neha; Tajmul, Md-D.; Yadav, Savita

doi:10.1007/s12010-015-1532-3

Cited by 5 publications

(1 citation statement)

References 40 publications

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“…The fruit is extensively used as a medicinal vegetable in Asia and Africa for a variety of ailments. Alternative medicine is made from several components of this plant, including the fruit, seed, leaf, and root" [4]. Traditional uses of the fruit include cardioprotective, antidote, aphrodisiac, cardiotonic, diuretic, and general tonic properties.…”

Section: Introductionmentioning

confidence: 99%

In vitro Propagation of Lagenaria Siceraria: A Plant of the Cucurbitaceae Family with High Medicinal Value

Kabiru,

Njenga

2023

APRJ

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Background and Objective: Lagenaria siceraria is extensively grown as a vegetable crop in tropical and subtropical regions of the world as a source of food and for it’s medicinal potential. The purpose of this research was to determine the optimal conditions for plantlet regeneration of Lagenaria siceraria microshoots on various concentrations of benzyl amino purine and naphthalene acetic acid in vitro. Materials and Methods: The research was designed as a completely randomized design. Shoot tips of Lagenaria siceraria were surface sterilized with sodium hypochlorite(NaOCl) at different concentrations (0,3,4,5,6 and 7 mg/L) and cultured on Murashige and Skoog (MS) solidified medium supplemented with 6-benzylaminopurine (BAP) at different concentrations of (0,0.5,1.0,1.5,2.0,2.5 and 3.0mg/L) for shoot induction and the obtained shoots were transferred to MS medium containing naphthaleneacetic Acid(NAA) at different concentrations (0,0.5,1.0,1.5,2.0,2.5 AND 3.0mg/L) for root induction. Results: Surface disinfection was optimal after immersion of shoot tips and/or nodal explants in cleaning detergent (5 min), ethanol 70% (1 min), and sodium hypochlorite 5mg/L (15 min). MS medium supplemented with 2.0 mg/L BAP was most effective in inducing shoot multiplication (100%), with an average of 4,33 shoots per explant after 4 weeks. MS medium supplemented with 2.0 mg/L NAA was most appropriate for rooting (100%) with an average of 5.33 roots per shoot after 4 weeks. Conclusion: shoots sterilized with 5mg/L NaOCl and inoculated in MS medium supplemented with 2.0 mg/LBAP and 2.0 mg/L NAA were optimal conditions for the surface sterilization, shoot and root induction of Lagenaria siceraria shoots.

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Section: Introductionmentioning

confidence: 99%

In vitro Propagation of Lagenaria Siceraria: A Plant of the Cucurbitaceae Family with High Medicinal Value

Kabiru,

Njenga

2023

APRJ

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Biotechnology and pharmacological evaluation of Indian vegetable crop Lagenaria siceraria: an overview

Roopan

Rajeswari

Kalpana

et al. 2015

Appl Microbiol Biotechnol

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Bottle gourd (Lagenaria siceraria) belongs to the family Cucurbitaceae, which comprises about 118 genera and 825 species. It is an important vegetable crop of India, and its production is influenced by a number of factors viz., environmental, nutritional, cultural operation and use of plant growth regulators. Since, bottle gourd belongs to a medicinal family, it plays a major role in the treatment of several diseases related to the skin and heart. There are several organic chemical compounds including vitamin B complex, pectin, dietary soluble fibres, ascorbic acid, beta-carotene, amino acids and minerals which have been isolated from this species. Therefore, the bottle gourd is considered to have a great impact on therapeutic health benefits. Due to drastic industrialization and urbanization, most of the human beings are facing several ill effects which may lead to death at extreme cases. Hence, the major research area was said to be nanotechnology. Taking into consideration, we have combined nanotechnology field with waste source in the name of green synthesis and planned to cure several diseases, as most of the researchers focused their work on this and succeeded too. The present study is a complete review of L. siceraria that covers the ethnomedical uses, chemical constituents, and pharmacological profile. This study is mainly focused on the antibacterial, hepatoprotective, diuretic and anthelminthic activities.

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Proteomic Analysis of the Protein Expression Profile in the Mature Nigella sativa (Black Seed)

Alanazi

Benabdelkamel

Alfadda

et al. 2016

Appl Biochem Biotechnol

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Nigella sativa (N. sativa) seed has been used as an important nutritional flavoring agent and in traditional medicine for treating many illnesses since ancient times. Understanding the proteomic component of the seed may lead to enhance the understanding of its structural and biological functional complexity. In this study, we have analyzed its proteome profile based on gel-based proteome mapping technique that includes one-dimensional gel electrophoresis followed by liquid chromatography and tandem mass spectrometry strategy. We have not come across any such studies that have been performed in N. sativa seeds up to date. A total of 277 proteins were identified, and their functional, metabolic, and location-wise annotations were carried out using the UniProt database. The majority of proteins identified in the proteome dataset based on their function were those involved in enzyme catalytic activity, nucleotide binding, and protein binding while the major cellular processes included regulation of biological process followed by regulation of secondary biological process, cell organization and biogenesis, protein metabolism, and transport. The identified proteome was localized mainly to the nucleus then to the cytoplasm, plasma membrane, mitochondria, plastid, and others. A majority of the proteins were involved in biochemical pathways involving carbohydrate metabolism, amino acid and shikimate pathway, lipid metabolism, nucleotide, cell organization and biogenesis, transport, and defense processes. The identified proteins in the dataset help to improve our understanding of the pathways involved in N. sativa seed metabolism and its biochemical features and detail out useful information that may help to utilize these proteins. This study could thus pave a way for future further high-throughput studies using a more targeted proteomic approach.

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Proteomic Analysis of Mature Lagenaria siceraria Seed

Cited by 5 publications

References 40 publications

In vitro Propagation of Lagenaria Siceraria: A Plant of the Cucurbitaceae Family with High Medicinal Value

In vitro Propagation of Lagenaria Siceraria: A Plant of the Cucurbitaceae Family with High Medicinal Value

Biotechnology and pharmacological evaluation of Indian vegetable crop Lagenaria siceraria: an overview

Proteomic Analysis of the Protein Expression Profile in the Mature Nigella sativa (Black Seed)

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