Proteomic Analysis of Macrophage Differentiation

Csar, Xavier F.; Wilson, Nicholas J.; McMahon, Kerrie‐Ann; Marks, Denese C.; Beecroft, Tina; Ward, Alister C.; Whitty, Genevieve; Kanangasundarum, Varuni; Hamilton, John A.

doi:10.1074/jbc.m100213200

Cited by 29 publications

(6 citation statements)

References 50 publications

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“…The addition of M-CSF to osteoclast precursors induces the expression of the receptor for RANKL (RANK), which together with cell adherence allows differentiation to proceed (11,12). The main features of intracellular signaling by c-Fms and RANK have been elucidated: c-Fms is a transmembrane tyrosine-specific protein kinase receptor, whose intracellular signaling involves Src and mitogen-activated protein kinase Erk activation (13,14). RANK is a member of the TNF receptor superfamily (15), and, like other family members, signals to the activation of the transcription factors NFB and, through the kinase Jnk, c-Jun (Ref.…”

mentioning

confidence: 99%

Transcriptional Program of Mouse Osteoclast Differentiation Governed by the Macrophage Colony-stimulating Factor and the Ligand for the Receptor Activator of NFκB

Cappellen¹,

Luong-Nguyen²,

Bongiovanni³

et al. 2002

Journal of Biological Chemistry

172

119

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mentioning

confidence: 99%

Transcriptional Program of Mouse Osteoclast Differentiation Governed by the Macrophage Colony-stimulating Factor and the Ligand for the Receptor Activator of NFκB

Cappellen¹,

Luong-Nguyen²,

Bongiovanni³

et al. 2002

Journal of Biological Chemistry

172

119

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“…Stimulation of the differentiated cells with LPS did not produce a further significant shift in the assembly state of SHC1 with 38.4% in the assembled state (BH p -value = 0.522). SHC1 has been directly implicated as a signaling adaptor in monocyte to macrophage differentiation in previous studies. , …”

Section: Resultsmentioning

confidence: 95%

“…SHC1 has been directly implicated as a signaling adaptor in monocyte to macrophage differentiation in previous studies. 53,54 We next applied the Protein-Centric Analysis module of CCprofiler to the THP-1 data set. Overall, we observed feature-specific quantitative differences (absolute log2FC larger than 1, BH p-value less than 0.05) between SEC features for 540 proteins in the differentiated vs undifferentiated comparison and in 30 proteins for the stimulated vs differentiated comparison (Figure 4c and Supplementary Tables 9−11).…”

Section: Protein Complex Reorganization In Differentiated and Stimula...mentioning

confidence: 99%

Rapid Profiling of Protein Complex Reorganization in Perturbed Systems

et al. 2023

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Protein complexes constitute the primary functional modules of cellular activity. To respond to perturbations, complexes undergo changes in their abundance, subunit composition, or state of modification. Understanding the function of biological systems requires global strategies to capture this contextual state information. Methods based on cofractionation paired with mass spectrometry have demonstrated the capability for deep biological insight, but the scope of studies using this approach has been limited by the large measurement time per biological sample and challenges with data analysis. There has been little uptake of this strategy into the broader life science community despite its rich biological information content. We present a rapid integrated experimental and computational workflow to assess the reorganization of protein complexes across multiple cellular states. The workflow combines short gradient chromatography and DIA/SWATH mass spectrometry with a data analysis toolset to quantify changes in a complex organization. We applied the workflow to study the global protein complex rearrangements of THP-1 cells undergoing monocyte to macrophage differentiation and subsequent stimulation of macrophage cells with lipopolysaccharide. We observed substantial proteome reorganization on differentiation and less pronounced changes in macrophage stimulation. We establish our integrated differential pipeline for rapid and state-specific profiling of protein complex organization.

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“…Proteomic analysis of macrophage differentiation was performed in the murine hemopoietic FD/WT cells which with Y706 mutation can be differentiated into macrophages by induction of GM-CSF and FD/807 cells with a defect in differentiation . Results from proteomic analysis demonstrated that these two cells had common and distinct profiles of tyrosine phosphorylated proteins by the GM-CSF, of which three comigrated with p46/52 Shc . Transfection with a nontyrosine-phosphorylatable form of p46/52 Shc prevented GM-CSF -mediated macrophage differentiation.…”

Section: Introductionmentioning

confidence: 99%

Proteomics and Leukocytes: An Approach to Understanding Potential Molecular Mechanisms of Inflammatory Responses

2004

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Leukocytes play an important role in the progression of disease and leukocyte-derived proteins are associated with the pathogenesis of the disease. Leukocyte activation causes production of inflammatory mediators, over-expression of cell surface adhesion molecules, and an increase in migration and infiltration, phagocytosis, and degranulation, as well as receptor phosphorylation and signal transduction. An increasing number of studies on the application of leukocyte proteomics have appeared in mapping protein profiles of inflammatory cells, contributing to the understanding of potential mechanisms involved in leukocyte function. Together with improvements in proteomic technology in leukocyte research, leukocyte proteomic analysis becomes more simple, rapid, flexible, sensitive, and specific. This enables proteomic investigation of activated or non-activated leukocytes to be highly focused on defined suborgans or specific signaling pathways. Research in leukocyte proteomics is progressing from fingerprinting to functioning, human cell lines to primary leukocytes, non-activated cells to inflammatory mediator-stimulated cells, in vitro culture to in vivo challenge, and animal models to human disease. A number of newly identified proteins from leukocyte proteomics may offer new mechanism-orientated targets for drug discovery and development.

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Proteomic Analysis of Macrophage Differentiation

Cited by 29 publications

References 50 publications

Transcriptional Program of Mouse Osteoclast Differentiation Governed by the Macrophage Colony-stimulating Factor and the Ligand for the Receptor Activator of NFκB

Transcriptional Program of Mouse Osteoclast Differentiation Governed by the Macrophage Colony-stimulating Factor and the Ligand for the Receptor Activator of NFκB

Rapid Profiling of Protein Complex Reorganization in Perturbed Systems

Proteomics and Leukocytes: An Approach to Understanding Potential Molecular Mechanisms of Inflammatory Responses

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