Proteomic analysis of lysine acetylation reveals that metabolic enzymes and heat shock proteins may be potential targets for DSS-induced mice colitis

Global analysis of lysine acetylation in the brain cortex of K18‐hACE2 mice infected with SARS‐CoV‐2

“…Because many acetylated proteins belonged to multiple compartments, there were considerable overlaps among these major cellular components (Figure 1I). These results were different from the findings obtained in other species and tissues [7,9,13,17,18], showing the differences in the subcellular localization of acetylated proteins among different species and cell types.…”

“…In this study, the acetylomes of brain cortexes for K18‐ hACE2 mice with and without infection were detected and we identified 3829 acetylated sites in 1735 proteins. Compared with the number of acetylated proteins found in other mouse tissues and cell lines in previous studies [13–16, 18, 19], we found 473 newly identified acetylated proteins in our study (Figures S5A, B, and Table S2). KEGG analysis manifested that these proteins strongly took part in glutamatergic synapse and synaptic vesicle cycle (Figure S5C and Table S3) and were different from acetylated proteins in other tissues [13–16, 18].…”

“…Compared with the number of acetylated proteins found in other mouse tissues and cell lines in previous studies [13-16, 18, 19], we found 473 newly identified acetylated proteins in our study (Figures S5A, B, and Table S2). KEGG analysis manifested that these proteins strongly took part in glutamatergic synapse and synaptic vesicle cycle (Figure S5C and Table S3) and were different from acetylated proteins in other tissues [13][14][15][16]18]. The results manifested that the types of acetylated proteins differed among different tissues and participated in specific pathways.…”

“…Accordingly, a motif analysis determined that the KacH and KacN motifs were the most conservative sequences of the Kac site. A comparison of these motifs with the acetylated sequence motifs of other mouse tissues or cell lines [11,[13][14][15][16][17][18][19] and species [7][8][9]12] indicated that KacH and KacN motifs are both found in the mouse hippocampus [17] and that the KacH motif is also found in the testis and hepatocytes of mice, which suggested that different preferences for motifs of Kac sites in different species and tissues.…”

“…Protein lysine acetylation (Kac) modification is related to many key cellular processes, such as gene transcription, protein folding, signal transduction, autophagy, and metabolism, and is closely associated with neurological diseases such as AD [6,7]. The acetylomes of various organs and cell lines of humans, mice, rats, and even some plants have been reported [7][8][9][10][11][12][13][14][15][16][17][18][19][20], but the acetylome of COVID-19 patients or in any animal models of COVID-19, including K18-hACE2 mice, have not been analyzed. To explore the relationship between neurological consequences of COVID-19 and acetylation in the brain cortex, we performed the first investigation of acetylome in the cortex of K18-hACE2 mice infected or uninfected with SARS-CoV-2 and studied the differences between the SARS-CoV-2 infection group and the control group.…”