Proteomic analysis of liver tissue reveals Aeromonas hydrophila infection mediated modulation of host metabolic pathways in Labeo rohita

Nissa, Mehar Un; Pinto, Nevil; Ghosh, Biplab; Singh, Urvi; Goswami, Mukunda; Srivastava, Sanjeeva

doi:10.1016/j.jprot.2023.104870

Cited by 7 publications

(6 citation statements)

References 74 publications

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“…In our study, TLR-13 was upregulated in the EM of treated fish ( Table 1 ), unlikely it was downregulated in the liver of L. rohita treated with A.hydrophila [39] . TLRs are the important epidermal proteins of innate immune system that acts as a receptor and help in the recognition of pathogen-associated molecular patterns (PAMPs) [40] .…”

Section: Discussionmentioning

confidence: 53%

Proteomic profile of epidermal mucus from Labeo rohita reveals differentially abundant proteins after Aeromonas hydrophila infection

Ali,

Ullah,

Kamarulzaman

et al. 2023

Fish and Shellfish Immunology Reports

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Section: Discussionmentioning

confidence: 53%

Proteomic profile of epidermal mucus from Labeo rohita reveals differentially abundant proteins after Aeromonas hydrophila infection

Ali,

Ullah,

Kamarulzaman

et al. 2023

Fish and Shellfish Immunology Reports

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“…Tissue protein quantification and digestion were done as mentioned in our previous work (15). In brief, protein was quantified through BCA method using Bovine serum Albumin as standard.…”

Section: Methodsmentioning

confidence: 99%

“…Bacterial isolation and identification was done as reported in our previous work (13). In brief, tissue from a naturally co-infected fish was used for isolation of Ah strain (NCBI Accession no.…”

Section: Bacterial Challenge and Tissue Samplingmentioning

confidence: 99%

“…first line of defence in gills of Carassius auratus against Ah (12). With the tangent of liver tissue analysis, a proteomic study suggests that Ah infection modulates host metabolic pathways and innate immunity with identified markers such as Toll like receptors and C-lectins in Labeo rohita (13). Our study builds upon these efforts by employing holistic discovery-based and validation-based proteomic methods to unravel the pathogenesis of A. hydrophila in the gut tissue of Labeo rohita (rohu) .…”

Section: Introductionmentioning

confidence: 99%

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Proteomic analysis of gut inLabeo rohitareveals ECM as Key Player in host’s Response toAeromonas hydrophilaInfection

Nissa,

Pinto,

Ghosh

et al. 2024

Preprint

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In the aquaculture sector, one of the challenges include disease outbreaks such as bacterial infections, particularly fromAeromonas hydrophila(Ah), impacting both wild and farmed fish. In this study, we conducted a proteomic analysis of the gut tissue inLabeo rohitafollowingAhinfection to elucidate the protein alterations and its implications for immune response. Our findings reveal significant dysregulation in extracellular matrix (ECM) associated proteins duringAhinfection, with increased abundance of elastin and Collagen alpha-3(VI) contributing to matrix rigidity. Pathway and enrichment analysis of differentially expressed proteins (DEPs) highlights the involvement of ECM-related pathways, including Focal adhesions, Integrin cell surface interactions, and actin cytoskeleton organization.Focal adhesions, crucial for connecting intracellular actin bundles to the ECM, play a pivotal role in immune response during infections. Increased abundance of integrin alpha 1, integrin beta 1, and Tetraspanin suggests their involvement in the host’s response toAhinfection. Proteins associated with actin cytoskeleton reorganization, such as myosin, tropomyosin, and phosphoglucomutase, exhibit increased abundance, influencing changes in cell behavior. Additionally, upregulated proteins like LTBP1 and Fibrillin-2 contribute to TGF-β signaling and focal adhesion, indicating their role in immune regulation.The study also identifies elevated levels of laminin, galectin 3, and tenascin-C, which interact with integrins and other ECM components, influencing immune cell migration and function. These proteins, along with decorin and lumican, act as immunomodulators, coordinating pro- and anti-inflammatory responses. ECM fragments released during pathogen invasion serve as “danger signals,” initiating pathogen clearance and tissue repair through Toll-like receptor signaling.IMPORTANCEThe study underscores the critical role of the extracellular matrix (ECM) and its associated proteins in the immune response of aquatic organisms during bacterial infections likeAeromonas hydrophila(Ah). Understanding the intricate interplay between ECM alterations and immune response pathways provides crucial insights for developing effective disease control strategies in aquaculture. By identifying key proteins and pathways involved in host defense mechanisms, this research lays the groundwork for targeted interventions to mitigate the impact of bacterial infections on fish health and aquaculture production.

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“…The application of proteomics in different aspects of aquaculture has been extensively reviewed in comprehensive papers, and its advantages and drawbacks are also discussed [ 22 , 23 , [101] , [102] , [103] , [104] , [105] ]. Proteomics as an analytical tool has been widely used in fish biology studies for exploring various aspects such as reproduction [ 106 , 107 ], stress response [108] , [109] , [110] , chromosomal manipulation [111] , [112] , [113] , nutrition [114] , [115] , [116] , toxicology [117] , [118] , [119] , disease and malformation [ 120 , 121 ], and tissue proteome profiling [ 122 , 123 ]. However, very few proteomic studies have been carried out to evaluate the effects of nano-Se on fish species to date.…”

Section: Application Of Proteomics In Nano-se Researchmentioning

confidence: 99%

A review of the quantitative real-time PCR and Omics approaches applied to study the effects of dietary selenium nanoparticles (nano-Se) on fish

Keyvanshokooh

2024

Comparative Immunology Reports

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Proteomic analysis of liver tissue reveals Aeromonas hydrophila infection mediated modulation of host metabolic pathways in Labeo rohita

Cited by 7 publications

References 74 publications

Proteomic profile of epidermal mucus from Labeo rohita reveals differentially abundant proteins after Aeromonas hydrophila infection

Proteomic profile of epidermal mucus from Labeo rohita reveals differentially abundant proteins after Aeromonas hydrophila infection

Proteomic analysis of gut inLabeo rohitareveals ECM as Key Player in host’s Response toAeromonas hydrophilaInfection

A review of the quantitative real-time PCR and Omics approaches applied to study the effects of dietary selenium nanoparticles (nano-Se) on fish

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