Proteomic analysis of ischemic rat brain after human mesenchymal stem cell transplantation

Li, Wen Yu; Jin, Ri Long; Hu, Xing; Chen, Wei; Bang, Oh Young

doi:10.1007/s13770-014-0048-1

Cited by 4 publications

(5 citation statements)

References 23 publications

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“…In order to understand the pathological mechanisms of ischemic stroke, factors have been investigated in neurovascular unit (comprising elements of the blood-brain barrier, myocytes, pericytes, extracellular matrix, astrocytes, and neurons) [3,4]. Potential biomarkers of cerebral ischemia have been explored such as DNA [5], RNA [6], microRNAs (miRNAs) [7][8][9][10], proteins [11,12], and metabolites in patients' plasma [13,15] or in brain tissue of rats with middle cerebral artery occlusion (MCAo) [14]. For example, elevated plasma DNA concentrations (>2500 kilogenome-equivalents/L) were detected in patients with hemorrhagic strokes compared with non-hemorrhagic strokes, with a 31% sensitivity and 83% specificity for discriminating the two types of stroke [5].…”

Section: Approaches To Diagnosis and Investigation Of Cerebral Ischemiamentioning

confidence: 99%

“…Protein biomarkers for endothelial damage, brain injury, inflammation, and coagulation/thrombosis were categorized and used to discriminate hemorrhagic stroke from ischemic stroke patients [12]. In the case of protein biomarkers in the rat MCAo model, different expression patterns of annexin A3 and synaptosomal-associated protein-25 were detected in brain tissue using 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) [11].…”

Section: Approaches To Diagnosis and Investigation Of Cerebral Ischemiamentioning

confidence: 99%

“…Yet, many patients do not seek or have access to such treatments in time, leaving them with significant brain damage and disability. Therefore, understanding the pathological mechanisms and metabolic biomarkers of cerebral ischemia have been pursued using patient samples, animal models, and in vitro experiments [ 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Metabolome Changes in Cerebral Ischemia

Shin

Lee

Basith

et al. 2020

Cells

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Cerebral ischemia is caused by perturbations in blood flow to the brain that trigger sequential and complex metabolic and cellular pathologies. This leads to brain tissue damage, including neuronal cell death and cerebral infarction, manifesting clinically as ischemic stroke, which is the cause of considerable morbidity and mortality worldwide. To analyze the underlying biological mechanisms and identify potential biomarkers of ischemic stroke, various in vitro and in vivo experimental models have been established investigating different molecular aspects, such as genes, microRNAs, and proteins. Yet, the metabolic and cellular pathologies of ischemic brain injury remain not fully elucidated, and the relationships among various pathological mechanisms are difficult to establish due to the heterogeneity and complexity of the disease. Metabolome-based techniques can provide clues about the cellular pathologic status of a condition as metabolic disturbances can represent an endpoint in biological phenomena. A number of investigations have analyzed metabolic changes in samples from cerebral ischemia patients and from various in vivo and in vitro models. We previously analyzed levels of amino acids and organic acids, as well as polyamine distribution in an in vivo rat model, and identified relationships between metabolic changes and cellular functions through bioinformatics tools. This review focuses on the metabolic and cellular changes in cerebral ischemia that offer a deeper understanding of the pathology underlying ischemic strokes and contribute to the development of new diagnostic and therapeutic approaches.

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Section: Approaches To Diagnosis and Investigation Of Cerebral Ischemiamentioning

confidence: 99%

Section: Approaches To Diagnosis and Investigation Of Cerebral Ischemiamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolome Changes in Cerebral Ischemia

Shin

Lee

Basith

et al. 2020

Cells

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“…The effectiveness of ADMSCs in treating IS was demonstrated in a middle cerebral artery occlusion (MCAO) rat model [ 17 ]. The modulating effect of human MSCs on endoplasmic reticulum (ER) stress was also validated in an MCAO rat model [ 19 ], strongly suggesting that treatment with MSCs alters inflammatory responses during an IS attack.…”

Section: Introductionmentioning

confidence: 99%

Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model

et al. 2018

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BackgroundThe current study was designed to explore the pathway through which adipose-derived mesenchymal stem cells (ADMSCs) affect brain ischemic injury.Material/MethodsThe improving effect of ADMSCs on the brain function and structure was evaluated in a middle cerebral artery occlusion (MCAO) rat model. The permeability of the brain-blood barrier (BBB), inflammatory response, and endoplasmic reticulum (ER) stress-related signaling induced by ischemia were determined.ResultsThe administration of ADMSCs decreased neurological severity score when compared with that in the MCAO group and also restricted the brain infarction area as well as cell apoptosis. ADMSCs suppressed the inflammation in brains by decreasing the expressions of IL-1β, IL-6, and TNF-α, contributing to the decreased permeability of the BBB. The expressions of pro-apoptosis factors in ER stress were inhibited while that of anti-apoptosis factors were induced.ConclusionsADMSCs affected brain injury in multiple ways, not only by suppressing inflammation in the brain infarction area, but also by blocking ER stress-induced apoptosis.

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“…Very few studies have reported global protein expression changes in the human or rat ischemic brain369). Monitoring following various kinds of cell therapy uses two-dimensional (2-D) electrophoresis-based protein expression analysis121422). After the 2-D electrophoresis, distinct messenger ribonucleic acid (mRNA)-protein expression have evaluated by quantitative methods1924).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of a Rat Cerebral Ischemic Injury Model after Human Cerebral Endothelial Cell Transplantation

Choi

Yun

Lee

et al. 2016

J Korean Neurosurg Soc

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ObjectiveCerebral endothelial cells have unique biological features and are fascinating candidate cells for stroke therapy.MethodsIn order to understand the molecular mechanisms of human cerebral endothelial cell (hCMEC/D3) transplantation in a rat stroke model, we performed proteomic analysis using 2-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Protein expression was confirmed by quantitative real-time PCR and Western blot.ResultsSeveral protein spots were identified by gel electrophoresis in the sham, cerebral ischemia (CI), and CI with hCMEC/D3 treatment cerebral ischemia with cell transplantation (CT) groups, and we identified 14 differentially expressed proteins in the CT group. Proteins involved in mitochondrial dysfunction (paraplegin matrix AAA peptidase subunit, SPG7), neuroinflammation (peroxiredoxin 6, PRDX6), and neuronal death (zinc finger protein 90, ZFP90) were markedly reduced in the CT group compared with the CI group. The expression of chloride intracellular channel 4 proteins involved in post-ischemic vasculogenesis was significantly decreased in the CI group but comparable to sham in the CT group.ConclusionThese results contribute to our understanding of the early phase processes that follow cerebral endothelial cell treatment in CI. Moreover, some of the identified proteins may present promising new targets for stroke therapy.

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Proteomic analysis of ischemic rat brain after human mesenchymal stem cell transplantation

Cited by 4 publications

References 23 publications

Metabolome Changes in Cerebral Ischemia

Metabolome Changes in Cerebral Ischemia

Tail Vein Infusion of Adipose-Derived Mesenchymal Stem Cell Alleviated Inflammatory Response and Improved Blood Brain Barrier Condition by Suppressing Endoplasmic Reticulum Stress in a Middle Cerebral Artery Occlusion Rat Model

Proteomic Analysis of a Rat Cerebral Ischemic Injury Model after Human Cerebral Endothelial Cell Transplantation

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