Proteomic analysis of <i>Strongyloides stercoralis</i> L3 larvae

Marcilla, Antonio; Sotillo, Javier; Pérez-García, Ana; Igual-Adell, Rafael; Valero, M. Luz; Sánchez-Pino, M. M.; Bernal, Dolores; Muñoz-Antolí, Carla; Trelis, María; Toledo, Rafael; Esteban, J. Guillermo

doi:10.1017/s0031182010000314

Cited by 36 publications

(35 citation statements)

References 29 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…myosin heavy chain, actin, tropomyosin, tubulin or paramyosin), metabolic enzymes (i.e. Trehalase, PEPCK, PGK, PGM, GAPDH, enolase), proteases, stress-response proteins, detoxifying enzymes, proteaseome components, most of them identified previously in S. stercoralis by proteomic studies [55]. These ES proteins play a major role in infection since they are present at the host-parasite interface and regulate host immune system [66].…”

Section: Discussionmentioning

confidence: 98%

“…With the exception of three metabolic proteins (citrate synthase, arginine kinase and ATP:guanido phosphotransferase) all proteins identified in a previous proteomic study with S. stercoralis L3i [55] were included in the transcriptome described here. In addition, 41 antigenic proteins including SiR and tropomyosin were present in the transcriptome (as searched in Table S1), confirming its value as a tool for searching targets for immunodiagnosis.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease

et al. 2012

View full text Add to dashboard Cite

Background Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions. Principal Findings Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti , revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified. Conclusions Overall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis , as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Proteomic analysis of S. stercoralis larvae identified the Ss-IR antigen after a short incubation in trypsin, suggesting that the antigen was on the larval surface [33]. However, using electron microscopy it was determined that IgG from mice immunized with Ss-IR bound to granules in the glandular esophagus.…”

Section: Discussionmentioning

confidence: 99%

Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice

Abraham¹,

Hess²,

Mejia³

et al. 2011

Vaccine

View full text Add to dashboard Cite

Human intestinal infections with the nematode Strongyloides stercoralis remain a significant problem worldwide and a vaccine would be a useful addition to the tools available to prevent and control this infection. The goal of this study was to test single antigens for their efficacy in a vaccine against S. stercoralis larvae in mice. Alum was used as the adjuvant in these studies and antigens selected for analysis were either recognized by protective human IgG (Ss-TMY-1, Ss-EAT-6, and Ss-LEC-5) or were known to be highly immunogenic in humans (Ss-NIE-1 and Ss-IR). Only mice immunized with the Ss-IR antigen demonstrated a significant decrease of approximately 80% in the survival of larval parasites in the challenge infection. Antibodies, recovered from mice with protective immunity to S. stercoralis after immunization with Ss-IR, were used to locate the antigen in the larvae. Confocal microscopy revealed that IgG from mice immunized with Ss-IR bound to the surface of the parasites and observations by electron microscopy indicated that IgG bound to granules in the glandular esophagus. Serum collected from mice immunized with Ss-IR passively transferred immunity to naïve mice. These studies demonstrate that Ss-IR, in combination with alum, induces high levels of protective immunity through an antibody dependent mechanism and may therefore be suitable for further development as a vaccine against human strongyloidiasis.

show abstract

“…Immunoblot analysis has been reported as a method for detection of the specific antibody against various antigenic polypeptide bands (Sato et al 1990;Northern and Grove 1990;Conway et al 1993Conway et al , 1994Atkins et al 1999;Sudre et al 2007). Recently, proteomic analysis for helminth proteins, such as those of S. stercoralis third-stage larvae (L3), have become available (Marcilla et al 2010). However, there is a lack of knowledge regarding antigenic proteins related to human strongyloidiasis sera.…”

Section: Introductionmentioning

confidence: 99%

Strongyloides stercoralis diagnostic polypeptides for human strongyloidiasis and their proteomic analysis

et al. 2016

View full text Add to dashboard Cite

Human strongyloidiasis is a deleterious gastrointestinal disease mainly caused by Strongyloides stercoralis infection. Strongyloides stercoralis is a soil-transmitted helminthiasis that is distributed around the globe. Although definitive diagnosis is carried out through the detection of parasite objects in human stool samples, the development of reliable immunological assays is an important alternative approach for supportive diagnosis. We characterized the two sensitive and specific bands of S. stercoralis filariform larvae that reacted with human strongyloidiasis sera based on immunoblot analysis. Serum samples obtained from strongyloidiasis patients showed a sensitivity of 90 and 80 % at the approximate molecular mass of 26 and 29-kDa polypeptide bands, respectively. The reactive specificity of the 26-kDa band was 76.5 % while for the 29-kDa band was 92.2 %. Proteomic analysis identified the 26-kDa band protein was 14-3-3 protein zeta, while the 29-kDa band protein was ADP/ATP translocase 4. The results provided a basic framework for further studies regarding the potential of the S. stercoralis recombinant antigen to become a leading to diagnostic tool.

show abstract

Proteomic analysis of Strongyloides stercoralis L3 larvae

Cited by 36 publications

References 29 publications

The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease

The Transcriptome Analysis of Strongyloides stercoralis L3i Larvae Reveals Targets for Intervention in a Neglected Disease

Immunization with the recombinant antigen Ss-IR induces protective immunity to infection with Strongyloides stercoralis in mice

Strongyloides stercoralis diagnostic polypeptides for human strongyloidiasis and their proteomic analysis

Contact Info

Product

Resources

About