Proteomic analysis of human serum for Finding pathogenic factors and potential biomarkers in preeclampsia

Abstract

Objective(s)-To apply a novel proteomic method to discover potential pathogenic factors and biomarkers of preeclampsia.Study design-Sera from five patients complicated with preeclampsia and five healthy pregnant controls were separately pooled. Each pool was treated with peptide ligand library beads (PLLBs) to remove high abundance proteins by affinity and thus enrich low abundance proteins. The proteins from the eluate were analyzed by a combination of 1D-Gel-LC-MS/MS. Protein expression levels were quantified using spectral counts and the extracted ion current.Results-1172 unique proteins in preeclampsia and 1149 in healthy controls were identified in the present study. 51 proteins were differentially expressed between preeclampsia and healthy pregnant women including chorionic somatommammptropin hormone (CSH) and fibulin-1. 31 proteins identified were up-regulated and 20 were down-regulated.Conclusions-The results demonstrate that peptide ligand library combining with 1D gel-LC-MS/MS analysis is an efficient method to identify differentially expressed proteins in sera and two biological processes of complement and coagulation activations and lipid metabolism were involved in the pathogenesis of preeclampsia.

Fibulin-1 Predicts Disease Progression in Patients With Idiopathic Pulmonary Fibrosis

“…Plasma levels of fi bulin-1 were identifi ed as a potential marker for kidney malfunction, 19 and increased levels of fi bulin-1 were detected in sera from patients with preeclampsia. 20 It is likely that the feature common to these diseases and the lung disease we studied is active fi brosis.…”

Maternal Mineral and Bone Metabolism During Pregnancy, Lactation, and Post-Weaning Recovery

“…However, plasma concentrations and placental expression of placental lactogen are not reduced in preeclamptic women (73,74,561,595,623,675,1027).…”

Mark Twain: How to fathom the depth of your pet proteome

“…However, if plasma is pre-treated with ProteoMiner, species can be detected down to as low as 10 pg protein per mL, with an increment of sensitivity of up to three orders of magnitude. Clear advantages in accessing the low-abundance plasma proteome via CPLL treatment have also been reported by Beseme et al [31] and other groups [16,[32][33][34][35][36][37][38] who claimed the importance of early-stage biomarkers. Although after a few years of use CPLLs did not yet generate validated protein markers, a number of scientific reports place a realistic discovery/validation hope very high.…”