Proteomic Analysis of Human Saliva From Lung Cancer Patients Using Two-Dimensional Difference Gel Electrophoresis and Mass Spectrometry

Xiao, Hua; Zhang, Lei; Zhou, Hui; Lee, Jay M.; Garon, Edward B.; Wong, David T.

doi:10.1074/mcp.m111.012112

Cited by 162 publications

(111 citation statements)

References 43 publications

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“…A modified version of 2DE by fluorescent tagging of proteins (DIGE) offers relatively increased throughput, ease of use, reproducibility and accurate quantitation of differential protein expression on a single gel [38]. In a recent study, proteomic analysis of human saliva from lung cancer patients revealed 16 candidate biomarkers [39].…”

Section: Technologies Involved In Proteomicsmentioning

confidence: 99%

Serum-based protein biomarkers for detection of lung cancer

2014

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Lung cancer is one of the most common cancers in terms of both incidence and mortality.The major reasons for the increasing number of deaths from lung cancer are late detection and lack of effective therapies. To improve our understanding of lung cancer biology, there is urgent need for blood-based, non-invasive molecular tests to assist in its detection in a cost-effective manner at an early stage when curative interventions are still possible. Recent advances in proteomic technology have provided extensive, high throughput analytical tools for identification, characterization and functional studies of proteomes. Changes in protein expression patterns in response to stimuli can serve as indicators or biomarkers of biological and pathological processes as well as physiological and pharmacological responses to drug treatment, thus aiding in early diagnosis and prognosis of disease. However, only a few biomarkers have been approved by the FDA to date for screening and diagnostic purposes. This review provides a brief overview of currently available proteomic techniques, their applications and limitations and the current state of knowledge about important serum biomarkers in lung cancer and their potential value as prognostic and diagnostic tools.

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Section: Technologies Involved In Proteomicsmentioning

confidence: 99%

Serum-based protein biomarkers for detection of lung cancer

2014

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“…Multiple targeting molecules have been studied in saliva (14)(15)(16)(17)(18)(19), and EFIRM has been applied in a pancreatic cancer rodent model for biomarker development (16). Here we describe the optimization of EFIRM for detecting EGFR mutations.…”

mentioning

confidence: 99%

Noninvasive Saliva-based EGFR Gene Mutation Detection in Patients with Lung Cancer

Fang¹,

Lin

Joon

et al. 2014

Am J Respir Crit Care Med

153

161

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Rationale: Constitutive activation of the epidermal growth factor receptor (EGFR) is prevalent in epithelial cancers, particularly in non-small cell lung carcinoma (NSCLC). Mutations identified in EGFR predict the sensitivity to EGFR-targeted therapy. Detection of these mutations is mainly based on tissue biopsy, which is invasive, expensive, and time consuming.Objectives: Noninvasive, real-time, inexpensive detection and monitoring of EGFR mutations in patients with NSCLC is highly desirable.Methods: We developed a novel core technology, electric field-induced release and measurement (EFIRM), which relies on a multiplexible electrochemical sensor that can detect EGFR mutations directly in bodily fluids.Measurements and Main Results: We established EFIRM for the detection of the EGFR mutations in vitro and correlated the results with tumor size from xenografted mice. In clinical application, we demonstrated that EFIRM could detect EGFR mutations in the saliva and plasma of 22 patients with NSCLC. Finally, a blinded test was performed on saliva samples from 40 patients with NSCLC. The receiver operating characteristic analysis indicated that EFIRM detected the exon 19 deletion with an area under the curve of 0.94 and the L858R mutation with an area under the curve of 0.96.Conclusions: Our data indicate that EFIRM is effective, accurate, rapid, user-friendly, and cost effective for the detection of EGFR mutations in the saliva of patients with NSCLC. We termed this saliva-based EGFR mutation detection (SABER).

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“…Salivary biomarkers are used for screening purposes in epidemiological studies [13] and are being used to monitor and detect various diseases, such as breast cancer [14], lung cancer [15], celiac disease [16], and chronic renal failure [11]. With regard to oral health, three important salivary variables should be investigated: pH, flow velocity, and buffering capacity.…”

Section: Discussionmentioning

confidence: 99%

Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças

Buche¹,

Gusso²,

Torres³

et al. 2017

Rev. Odonto Ciênc. (Online)

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OBJECTIVE: Human saliva has numerous components that are important for maintaining oral health. We investigated the association between iron levels in saliva in children and their caries experience. METHODS: We conducted a case-control study that included 186 healthy children, aged 11-14 years. The decayed-filled teeth/decayed-missing-filled teeth (DMFT) score was estimated according to the Brazilian Oral Health Project. The children were divided into two groups: caries-free group (DMFT=0, n=55) and caries experience group (DMFT≥1, n=131). Saliva was collected from each child using the spitting method and analyzed for stimulated saliva flow (SSF), pH, and iron levels. The SSF, pH, and salivary iron levels were analyzed for statistical assumptions of normality and homoscedasticity and compared between groups. All of the statistical tests were performed using R and SPSS software, with a level of significance of 0.05. RESULTS: No statistically significant differences in mean pH were found between groups (t=-0.0488, p=0.51). Children with caries experience had significantly lower salivary iron levels than caries-free children (w=5088, p<0.0001). The SSF values were significantly different between children with caries experience and children without caries (w=4198, p=0.03). CONCLUSION: Salivary iron levels were significantly lower in children with dental caries experience, suggesting that salivary iron plays a role in maintaining oral health.Key words: Saliva; Salivary iron; Caries Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças RESUMO OBJETIVO: Investigar a associação entre os níveis de ferro na saliva das crianças e sua experiência de cárie. MÉTODOS: Realizamos um estudo caso-controle envolvendo 186 crianças saudáveis de 11 a 14 anos. O número de dentes cariados, perdidos e obturados (CPO-D) foi estimado de acordo com o Projeto Saúde Bucal Brasil (SBBrasil) e as crianças foram divididas em dois grupos: grupo sem cárie (CPO-D=0; n=55) e experiência Grupo de cárie (CPO-D≥1; n=131). A saliva foi coletada de cada indivíduo usando o método de cuspir e analisada quanto ao fluxo estimado de saliva (SSF), pH e concentração de ferro. As variáveis SSF, pH e ferro salivar foram analisadas pelos pressupostos estatísticos de normalidade e homocedasticidade e comparados entre os grupos. Todos os testes foram realizados utilizando os programas estatísticos R (R Core Team, 2016) e SPSS (IBM, EUA), assumindo um nível de significância de 0,05. RESULTADOS: Não houve diferenças estatisticamente significativas entre os meios de pH entre os grupos (t=-0,0488; p=0,51). Em relação ao ferro salivar, as crianças com experiência em cárie apresentaram valores significativamente mais baixos de ferro salivar do que aqueles que não apresentavam caries (w=5088; p<0,0001). Além disso, os valores de SSF foram diferentes entre as crianças com experiência de cárie e aqueles sem cárie (w=4198; p=0,03). CONCLUSÃO: os níveis de ferro salivar foram estatisticamente inferiores em crianças com cárie den...

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Proteomic Analysis of Human Saliva From Lung Cancer Patients Using Two-Dimensional Difference Gel Electrophoresis and Mass Spectrometry

Cited by 162 publications

References 43 publications

Serum-based protein biomarkers for detection of lung cancer

Serum-based protein biomarkers for detection of lung cancer

Noninvasive Saliva-based EGFR Gene Mutation Detection in Patients with Lung Cancer

Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças

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