Proteomic analysis of human omental adipose tissue in the polycystic ovary syndrome using two-dimensional difference gel electrophoresis and mass spectrometry

Cortón, Marta; Botella-Carretero, José I.; López, Juan Antonio; Camafeita, Emilio; Millán, José L. San; Escobar-Morreale, Héctor F.; Peral, Belén

doi:10.1093/humrep/dem380

Cited by 101 publications

(103 citation statements)

References 61 publications

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“…In this study, 2D-DIGE revealed significantly lower A1AT and ANXA5 in adipose tissue from GDM subjects, suggesting increased inflammation in this tissue. However, in contrast to our studies, ANXA5 was found to be overexpressed in PCOS omental adipose tissue compared with those of non-hyperandrogenic women (Corton et al 2008). …”

Section: Discussioncontrasting

confidence: 99%

“…Thus, the decrease in PRX6, HSP27, GSTP and SBP1 in adipose tissue from women with GDM compared with normal tissue is suggestive of increased oxidative stress. In support of our studies, 2D-DIGE also revealed that the antioxidant enzyme PRX2 was found to be down-regulated in PCOS omental fat (Corton et al 2008).…”

Section: Oxidative Stresssupporting

confidence: 88%

“…For example, proteomic analysis revealed altered expression levels of several proteins that are involved in lipid and glucose metabolism, inflammation and oxidative stress processes in polycystic ovary syndrome (PCOS) omental adipose tissue in comparison with those of non-hyperandrogenic obese women (Corton et al 2008). Differences regarding these studies and our studies are also discussed below.…”

Section: Discussionmentioning

confidence: 76%

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2D-DIGE to identify proteins associated with gestational diabetes in omental adipose tissue

Oliva

Barker

Rice

et al. 2013

Journal of Endocrinology

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Gestational diabetes mellitus (GDM) is a significant risk factor for the type 2 diabetes epidemic in many populations. Maternal adipose tissue plays a central role in the pathophysiology of GDM. Thus, the aim of this study was to determine the effect of GDM on the proteome of adipose tissue. Omental adipose tissue was obtained at the time of term Caesarean section from women with normal glucose tolerance (NGT) or GDM. 2D-difference gel electrophoresis (DIGE), followed by mass spectrometry, was used to identify protein spots (nZ6 patients per group). Western blotting was used for confirmation of six of the spot differences (nZ6 patients per group). We found 14 proteins that were differentially expressed between NGT and GDM adipose tissue (R1.4-fold, P!0.05). GDM was associated with an up-regulation of four proteins: collagen alpha-2(VI) chain (CO6A2 (COL6A2)), fibrinogen beta chain (FIBB (FGB)), lumican (LUM) and S100A9. On the other hand, a total of ten proteins were found to be down-regulated in adipose tissue from GDM women. These were alpha-1-antitrypsin (AIAT (SERPINA1)), annexin A5 (ANXA5), fatty acid-binding protein, adipocyte (FABP4), glutathione S-transferase P (GSTP (GSTP1)), heat-shock protein beta-1 (HSP27 (HSPB1)), lactate dehydrogenase B chain (LDHB), perilipin-1 (PLIN1), peroxiredoxin-6 (PRX6 (PRDX6)), selenium-binding protein 1 (SBP1) and vinculin (VINC (VCL)). In conclusion, proteomic analysis of omental fat reveals differential expression of several proteins in GDM patients and NGT pregnant women. This study revealed differences in expression of proteins that are involved in inflammation, lipid and glucose metabolism and oxidative stress and added further evidence to support the role of visceral adiposity in the pathogenesis of GDM.

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Section: Discussioncontrasting

confidence: 99%

Section: Oxidative Stresssupporting

confidence: 88%

Section: Discussionmentioning

confidence: 76%

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2D-DIGE to identify proteins associated with gestational diabetes in omental adipose tissue

Oliva

Barker

Rice

et al. 2013

Journal of Endocrinology

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“…Evidence taken from the literature (20,23,24,97) as well as from our group (A. O'Connor, M. Morine, N. Phelan, G. Boran, J. Gibney and H. M. Roche, unpublished results) suggests that a degree of adipose tissue dysfunction is present within the PCOS population. Therefore the therapeutic role of n-3 PUFA supplementation in the adipose tissue was considered of prime concern.…”

Section: Proceedings Of the Nutrition Societymentioning

confidence: 56%

Metabolic and hormonal aspects of polycystic ovary syndrome: the impact of diet

2010

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Polycystic ovary syndrome (PCOS) is a common, chronic endocrine condition affecting young women of reproductive age. It is characterised by hyperandrogenaemia, and profound menstrual and ovulatory dysfunction with consequent sub-fertility. A clustering of metabolic aberrations is commonly associated with this condition and these include insulin resistance, disordered lipid metabolism and chronic low-grade inflammation. Overweight and obesity, as well as a degree of adipose tissue dysfunction, are present in a large proportion of women with PCOS, and where present, magnify the inherent hyperandrogenaemia characteristic of the condition, in addition to worsening the metabolic profile. Diet and lifestyle interventions are among the first-line treatments for PCOS, and weight reduction through energy restriction has been shown to exert positive influences on both metabolic and hormonal aspects of this condition. Alterations in carbohydrate amount and type have also been investigated, and more recently, dietary fatty acids, with a particular emphasis on PUFA, have been shown to have a positive impact within this population group. Although it is likely that diet is not the root cause of PCOS, it represents a modifiable variable with the potential to improve the health of women with this condition. Work to date has provided insights into the role of diet in PCOS; however, further work is required to determine the role of nutrients specifically within the context of PCOS, in order to develop more effective, evidence-based dietary guidelines for this condition.

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“…Peroxiredoxin-2 has an antioxidant activity and its down-regulation in both PCOS and T2DM indicates oxidative stress and toxicity processes (45). In fact, oxidative stress has been suggested to occur in many disorders related to insulin resistance like T2DM and PCOS (46).…”

Section: Discussionmentioning

confidence: 99%

MECHANISMS IN ENDOCRINOLOGY: Proteomic biomarkers of type 2 diabetes mellitus risk in women with polycystic ovary syndrome

Galazis

Afxentiou

Xenophontos

et al. 2013

European Journal of Endocrinology

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Women with polycystic ovary syndrome (PCOS) are at increased risk of developing insulin resistance and type 2 diabetes mellitus (T2DM). In this study, we attempted to list the proteomic biomarkers of PCOS and T2DM that have been published in the literature so far. We identified eight common biomarkers that were differentially expressed in both women with PCOS and T2DM when compared with healthy controls. These include pyruvate kinase M1/M2, apolipoprotein A-I, albumin, peroxiredoxin 2, annexin A2, a-1-B-glycoprotein, flotillin-1 and haptoglobin. These biomarkers could help improve our understanding of the links between PCOS and T2DM and could be potentially used to identify subgroups of women with PCOS at increased risk of T2DM. More studies are required to further evaluate the role these biomarkers play in women with PCOS and T2DM.European Journal of Endocrinology 168 R33-R43

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Proteomic analysis of human omental adipose tissue in the polycystic ovary syndrome using two-dimensional difference gel electrophoresis and mass spectrometry

Cited by 101 publications

References 61 publications

2D-DIGE to identify proteins associated with gestational diabetes in omental adipose tissue

2D-DIGE to identify proteins associated with gestational diabetes in omental adipose tissue

Metabolic and hormonal aspects of polycystic ovary syndrome: the impact of diet

MECHANISMS IN ENDOCRINOLOGY: Proteomic biomarkers of type 2 diabetes mellitus risk in women with polycystic ovary syndrome

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