Proteomic analysis of human mesenchymal stromal cells derived from adipose tissue undergoing osteoblast differentiation

Giusta, M.S.; Andrade, Hélida Monteiro de; Santos, Agenor Valadares; Castanheira, Paula; Lamana, L. Ferrando; Pimenta, Adriano M.C.; Góes, Alfredo M.

doi:10.3109/14653240903580270

Cited by 22 publications

(11 citation statements)

References 38 publications

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“…hMSC proteomics during bone differentiation has been characterized earlier in many studies, including our own previous work [35,[44][45][46]. Here, the aim was to specifically focus on precise characterization of the exocytosed proteins that are peripherally attached to cell surface as well as glycoproteoglycansins and to compare the plasma membrane attached to BMMSC and UCBMSC secretomes, which has not been previously done.…”

mentioning

confidence: 99%

Mitochondrial Function and Energy Metabolism in Umbilical Cord Blood- and Bone Marrow-Derived Mesenchymal Stem Cells

Pietilä

Palomäki

Lehtonen

et al. 2012

Stem Cells and Development

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Human mesenchymal stem cells (hMSCs) are an attractive choice for a variety of cellular therapies. hMSCs can be isolated from many different tissues and possess unique mitochondrial properties that can be used to determine their differentiation potential. Mitochondrial properties may possibly be used as a quality measure of hMSC-based products. Accordingly, the present work focuses on the mitochondrial function of hMSCs from umbilical cord blood (UCBMSC) cells and bone marrow cells from donors younger than 18 years of age (BMMSC <18) and those more than 50 years of age (BMMSC >50). Changes of ultrastructure and energy metabolism during osteogenic differentiation in all hMSC types were studied in detail. Results show that despite similar surface antigen characteristics, the UCBMSCs had smaller cell surface area and possessed more abundant rough endoplasmic reticulum than BMMSC >50. BMMSC <18 were morphologically more UCBMSC-like. UCBMSC showed dramatically higher mitochondrial-to-cytoplasm area ratio and elevated superoxide and manganese superoxide dismutase (MnSOD) levels as compared with BMMSC >50 and BMMSC <18. All hMSCs types showed changes indicative of mitochondrial activation after 2 weeks of osteogenic differentiation, and the increase in mitochondrial-to-cytoplasm area ratio appears to be one of the first steps in the differentiation process. However, BMMSC >50 showed a lower level of mitochondrial maturation and differentiation capacity. UCBMSCs and BMMSCs also showed a different pattern of exocytosed proteins and glycoproteoglycansins. These results indicate that hMSCs with similar cell surface antigen expression have different mitochondrial and functional properties, suggesting different maturation levels and other significant biological variations of the hMSCs. Therefore, it appears that mitochondrial analysis presents useful characterization criteria for hMSCs intended for clinical use.

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confidence: 99%

Mitochondrial Function and Energy Metabolism in Umbilical Cord Blood- and Bone Marrow-Derived Mesenchymal Stem Cells

Pietilä

Palomäki

Lehtonen

et al. 2012

Stem Cells and Development

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“…1c). The ability of ADSCs to differentiate into osteoblast cells has been previously reported [21,29]. In this study, a specific medium was used to induce differentiation.…”

Section: Resultsmentioning

confidence: 93%

Healing Pattern in Calvarial Bone Defects Following Bone Regeneration in Rats Guided by Chitosan Scaffold and Adipose Tissue-Derived Mesenchymal Stem Cells

Carvalho¹,

Breyner²,

Hell³

et al. 2012

TOTERMJ

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Cell-based tissue engineering using scaffolds provides a promising option for the repair of bone tissue damage caused by trauma or aging-related degeneration such as osteoporosis. In this study, a porous 3D scaffold was used to support the differentiation process of rat adipose -derived stem cells (ADSCs) into osteoblasts in vitro. The scaffold was made with chitosan, gelatin and chondroitin. In addition, the scaffold was crosslinked by glutaraldehyide. The osteogenic differentiation of ADSCs was improved in 3D culture as shown by the cell viability assay (MTT) and analyses of, alkaline phosphatase activity (ALP), and collagen production during three weeks of culturing. During the 2nd and 3rd weeks of culturing, bone markers, such as osteopontin and osteocalcin, were detected by the PCR analysis. In vivo biocompatibility was evaluated subcutaneously in rats. A mild inflammatory response was observed during the 5 weeks. Reduction of the matrix fibers by reabsorptive cells and formation of new blood vessels were observed during this period. However, no inflammation was observed. Five weeks after the implants were placed in the calvaria -defects, a small amount of bone repair was observed. In addition, immunohistochemistry revealed the presence of EGFPs ADSCs in the newly formed extracellular matrix. These findings indicated that the chitosan-gelatin-chondroitin 3D structure enhances cellular proliferation, which may be useful in the development of biomaterials for the stimulation of adult stem cells in bone tissue engineering.

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“…However, the process of osteogenic differentiation is still not completely understood at either the cellular or molecular level. Proteomic techniques may improve the study of phenotype alterations, and thus the understanding of osteogenic induction (Giusta et al, 2010). Given results of the present study, further investigations beyond SNP analyses, including proteomics and other environmental influences, are merited.…”

Section: Discussionmentioning

confidence: 99%

Association between BMP-2 and COL6A1 gene polymorphisms with susceptibility to ossification of the posterior longitudinal ligament of the cervical spine in Korean patients and family members

Kuh

et al. 2014

Genet. Mol. Res.

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ABSTRACT. COL6A1 and BMP-2 genes have been implicated in ossification of the posterior longitudinal ligament (OPLL) susceptibility in Japanese and Chinese Han populations. However, no study has yet investigated the DNA of unaffected family members of patients with OPLL. This study investigated differences in genetic polymorphisms of BMP-2 and COL6A1 between Korean patients with OPLL and their family members (with and without OPLL). A total of 321 subjects (110 patients with OPLL and 211 family members) were enrolled in the study. Associations between two single nucleotide polymorphisms (SNPs) of the BMP-2 gene (Ser37Ala and Ser87Ser) and two SNPs of COL6A1 [promoter (-572) and intron 33 (+20)] with susceptibility to OPLL of the cervical spine were investigated between the two groups (OPLL+ BMP-2 and COL6A1 polymorphisms and OPLL in Koreans and OPLL-). Of the 321 subjects, 162 had cervical OPLL (50.4%; 110 patients, 52 family members). There was a familial tendency of OPLL in 34 of the 110 families (30.9%). Allele and haplotype frequencies of the four SNPs in the BMP-2 and COL6A1 genes did not differ significantly between the OPLL+ and OPLL-groups, even when excluding participants over 50 years of age. This is the first report identifying SNPs of COL6A1 and BMP-2 in Korean patients and family members with OPLL. Although allele and haplotype frequencies were similar with those of a previous study in Japanese and Chinese patients, unaffected family members also showed similar rates of these SNPs in the present study. These results suggest that these SNPs may not directly influence the expression of OPLL.

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Proteomic analysis of human mesenchymal stromal cells derived from adipose tissue undergoing osteoblast differentiation

Cited by 22 publications

References 38 publications

Mitochondrial Function and Energy Metabolism in Umbilical Cord Blood- and Bone Marrow-Derived Mesenchymal Stem Cells

Mitochondrial Function and Energy Metabolism in Umbilical Cord Blood- and Bone Marrow-Derived Mesenchymal Stem Cells

Healing Pattern in Calvarial Bone Defects Following Bone Regeneration in Rats Guided by Chitosan Scaffold and Adipose Tissue-Derived Mesenchymal Stem Cells

Association between BMP-2 and COL6A1 gene polymorphisms with susceptibility to ossification of the posterior longitudinal ligament of the cervical spine in Korean patients and family members

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