Proteomic analysis of host cell protein fouling during bioreactor harvesting
Da Zhang,
S. Ranil Wickramasinghe,
Andrew L. Zydney
et al.
Abstract:Chinese hamster ovary (CHO) cells are among the most common cell lines used for therapeutic protein production. Membrane fouling during bioreactor harvesting is a major limitation for the downstream purification of therapeutic proteins. Host cell proteins (HCP) are the most challenging impurities during downstream purification processes. The present work focuses on identification of HCP foulants during CHO bioreactor harvesting using reverse asymmetrical commercial membrane BioOptimal™ MF‐SL. In order to inves… Show more
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