Proteomic analysis of high‐density lipoprotein

Rezaee, Farhad; Casetta, Bruno; Levels, J.H.M.; Speijer, Dave; Meijers, Joost C. M.

doi:10.1002/pmic.200500191

Cited by 161 publications

(169 citation statements)

References 36 publications

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“…The identities of the proteins attached to HDL, LDL, and VLDL have recently been analyzed using proteomics (10)(11)(12)(13)(14)(15)(16). In particular, the list of proteins believed to be associated with HDL particles has grown significantly in recent years (17).…”

Section: White) (D and E)mentioning

confidence: 99%

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts

Lundqvist

Stigler

Elia

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

2,614

2,340

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Nanoparticles in a biological fluid (plasma, or otherwise) associate with a range of biopolymers, especially proteins, organized into the ''protein corona'' that is associated with the nanoparticle and continuously exchanging with the proteins in the environment. Methodologies to determine the corona and to understand its dependence on nanomaterial properties are likely to become important in bionanoscience. Here, we study the long-lived (''hard'') protein corona formed from human plasma for a range of nanoparticles that differ in surface properties and size. Six different polystyrene nanoparticles were studied: three different surface chemistries (plain PS, carboxyl-modified, and amine-modified) and two sizes of each (50 and 100 nm), enabling us to perform systematic studies of the effect of surface properties and size on the detailed protein coronas. Proteins in the corona that are conserved and unique across the nanoparticle types were identified and classified according to the protein functional properties. Remarkably, both size and surface properties were found to play a very significant role in determining the nanoparticle coronas on the different particles of identical materials. We comment on the future need for scientific understanding, characterization, and possibly some additional emphasis on standards for the surfaces of nanoparticles.bionanoscience ͉ mass spectrometry ͉ interactions ͉ proteomics ͉ human plasma

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Section: White) (D and E)mentioning

confidence: 99%

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts

Lundqvist

Stigler

Elia

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

2,614

2,340

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“…HDL protein profiling is increasingly being used to determine the biochemical composition of HDL (20,27,28). During acute systemic inflammation HDL becomes proinflammatory, loses its protective properties, and can even enhance atherogenesis (12,29).…”

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confidence: 99%

Improvement of lipid profile is accompanied by atheroprotective alterations in high‐density lipoprotein composition upon tumor necrosis factor blockade: A prospective cohort study in ankylosing spondylitis

Eijk¹,

Vries²,

Levels³

et al. 2009

Arthritis & Rheumatism

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Objective. Cardiovascular mortality is increased in ankylosing spondylitis (AS), and inflammation plays an important role. Inflammation deteriorates the lipid profile and alters high-density lipoprotein cholesterol (HDL-c) composition, reflected by increased concentrations of serum amyloid A (SAA) within the particle. Anti-tumor necrosis factor (anti-TNF) treatment may improve these parameters. We therefore undertook the present study to investigate the effects of etanercept on lipid profile and HDL composition in AS.Methods. In 92 AS patients, lipid levels and their association with the inflammation markers C-reactive protein (CRP), erythrocyte sedimentation rate, and SAA were evaluated serially during 3 months of etanercept treatment. HDL composition and its relationship to inflammation markers was determined in a subgroup of patients, using surface-enhanced laser desorption/ ionization time-of-flight analysis.Results. With anti-TNF treatment, levels of all parameters of inflammation decreased significantly, whereas total cholesterol, HDL-c, and apolipoprotein A-I (Apo A-I) levels increased significantly. This resulted in a better total cholesterol:HDL-c ratio (from 3.9 to 3.7) (although the difference was not statistically significant), and an improved Apo B:Apo A-I ratio, which decreased by 7.5% over time (P ‫؍‬ 0.008). In general, increases in levels of all lipid parameters were associated with reductions in inflammatory activity. In addition, SAA was present at high levels within HDL particles from AS patients with increased CRP levels and disappeared during treatment, in parallel with declining plasma levels of SAA.Conclusion. Our results show for the first time that during anti-TNF therapy for AS, along with favorable changes in the lipid profile, HDL composition is actually altered whereby SAA disappears from the HDL particle, increasing its atheroprotective ability. These findings demonstrate the importance of understanding the role of functional characteristics of HDL-c in cardiovascular diseases related to chronic inflammatory conditions.

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“…Many lines of evidence strongly link them to the immune system and macrophage biology (91)(92)(93) . Several approaches have been developed for lipoprotein isolation (94)(95)(96)(97) ; delipidation (98,99) ; sample preparation (solubilisation and digestion) (100)(101)(102)(103) ; and the characterisation of protein component of lipoproteins using MS (104,105) .…”

Section: Analysis Of Post-translational Modificationsmentioning

confidence: 99%

Nutriproteomics: technologies and applications for identification and quantification of biomarkers and ingredients

Sénéchal

Kussmann

2011

Proc. Nutr. Soc.

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Nutrition refers to the process by which a living organism ingests and digests food and uses the nutrients therein for growth, tissue maintenance and all other functions essential to life. Food components interact with our body at molecular, cellular, organ and system level. Nutrients come in complex mixtures, in which the presence and concentration of single compounds as well as their interactions with other compounds and the food matrix influence their bioavailability and bioefficacy. Traditionally, nutrition research mainly concentrated on supplying nutrients of quality to nourish populations and on preventing specific nutrient deficiencies. More recently, it investigates health-related aspects of individual ingredients or of complete diets, in view of health promotion, performance optimisation, disease prevention and risk assessment. This review focuses on proteins and peptides, their role as nutrients and biomarkers and on the technologies developed for their analysis. In the first part of this review, we provide insights into the way proteins are currently characterised and analysed using classical and emerging proteomic approaches. The scope of the second part is to review major applications of proteomics to nutrition, from characterisation of food proteins and peptides, via investigation of health-related food benefits to understanding disease-related mechanisms.

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Proteomic analysis of high‐density lipoprotein

Cited by 161 publications

References 36 publications

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts

Improvement of lipid profile is accompanied by atheroprotective alterations in high‐density lipoprotein composition upon tumor necrosis factor blockade: A prospective cohort study in ankylosing spondylitis

Nutriproteomics: technologies and applications for identification and quantification of biomarkers and ingredients

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