Proteomic analysis of heterosis during maize seed germination

Fu, Zhiyuan; Jin, Xining; Ding, Dong; Li, Yongling; Fu, Zhongjun; Tang, Jihua

doi:10.1002/pmic.201000481

Cited by 63 publications

(63 citation statements)

References 64 publications

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“…Similar results were observed by Fu et al (2011), who compared proteomic analyses and heterosis during maize seed germination. These authors also concluded that heterosis in the hybrid results from the interaction between the effects of various proteins, which cannot be associated with only one protein.…”

Section: Discussionsupporting

confidence: 86%

“…There was a positive correlation between the gibberellin content found in the seedlings and the increases in growth rate, leaf area, and plant height of maize. Fu et al (2011) carried out proteomic analyses in seeds during germination and concluded that the capacity to repress abscisic acid in the hybrid seeds can in part explain the heterosis.…”

Section: Introductionmentioning

confidence: 99%

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Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

et al. 2015

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ABSTRACT. In this study, we analyzed heterosis, amylase enzyme gene expression, and the physiological quality of maize seeds with different genotypes and sizes, which were subjected to aging and not subjected to aging. We used seeds from 2 maize lines that differed with regard to physiological quality, the hybrid, and the reciprocal hybrid; they were classified into 2 sizes and were subjected to aging and not subjected to aging. Physiological quality was assessed by performing tests for germination, emergence, emergence speed index, and artificial aging. Expressions of the genes alpha amylase B73, alpha amylase (LOC542522), isoamylase mRNA clone 353244, and the endogenous controls ubiquitin and alcohol dehydrogenase in the seeds were studied using quantitative real-time-polymerase chain reaction. We observed heterosis for seed quality and for expression of amylase genes in the G.E. Oliveira et al. 8624©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8623-8633 (2015) genotypes studied. We found no difference in seed quality between large and small seeds.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

et al. 2015

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“…Several studies suggest that proteomic application between maize hybrids and their parents can identify posttranscriptional protein differences (Hoecker et al, 2008;Muthreich et al, 2010;Fu et al, 2011), and proteins related to heterosis during maize germination stages have been identified (Hoecker et al, 2008). The observed patterns indicate important roles of dominance, partial dominance and over-dominance in maize (Fu et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of muscle between hybrid abalone and parental lines Haliotis gigantea Reeve and Haliotis discus hannai Ino

Luo

Wang

et al. 2015

Heredity

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To understand the potential molecular mechanism of heterosis, protein expression patterns were compared from hybrids of Haliotis gigantea (G) and Haliotis discus hannai (D) using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight analyses. Expression differences were observed in muscle samples from the four groups with 673 ± 21.0 stained spots for H. discus hannai ♀ × H. discus hannai ♂ (DD), 692 ± 25.6 for H. gigantea ♀ × H. gigantea ♂ (GG), 679 ± 16.2 for H. discus hannai ♀ × H. gigantea ♂ (DG) (F 1 hybrid) and 700 ± 19 for H. gigantea ♀ × H. discus hannai ♂ (GD) (F 1 hybrid). Different 2-DE image muscle protein spots had a mirrored relationship between purebreds and the F 1 hybrid, suggesting that all stained spots in F 1 hybrid muscle were on 2-DEs from parents. DD and DG clustered together first, and then clustered with GD, whereas the distance of DD and GG was maximal according to hierarchical cluster analysis. We identified 136 differentially expressed protein spots involved in major biological processes, including energy metabolism and stress response. Most energy metabolism proteins were additive, and stress-induced proteins displayed additivity or overdominance. In these 136 identified protein spots, hybrid offspring with additivity or over-dominance accounted for 68.38%. Data show that a proteomic approach can provide functional prediction of abalone interspecific hybridization.

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“…Cupins belong to superfamily of functionally diverse proteins, including seed storage proteins, auxin binding proteins, enzymes, transcription factors, and stressrelated proteins, which are involved in seed germination and seedlings development (Dunwell et al 2004;Gong et al 2013). Fu et al (2011) suggested that cupin proteins, such as native germin and germin-like proteins, protected plant cells from the oxidative stress during seed germination.…”

Section: Resultsmentioning

confidence: 99%

Exogenous melatonin expediently modifies proteome of maize (Zea mays L.) embryo during seed germination

et al. 2016

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Melatonin (N-acetyl-5-methoxytryptamine) has a great potential for plant biostimulation. Its role in plant physiology is intensively explored, and its important function in plant stress defence frequently underlined. Melatonin is particularly effective when applied as an additional factor of seed priming. In the presented research, hydroconditioning was chosen experimentally as optimal for maize (Zea mays L.) seeds. The following seed variants were compared: controlled non-treated, hydroprimed with water and hydroprimed with melatonin solutions 50 and 500 lM. To identify modifications in proteome of maize seeds caused by the applied hydroconditioning techniques, protein extracts of germinated seed embryos (24 h, 25°C) were separated by 2D-PAGE. Next, obtained maps of proteomes were compared (statistically and graphically) using PDQuest software, and characteristic spots of proteins were analysed qualitatively by mass-spectrometric techniques and identified in the Mascot protein databases. Research helped to identify hydropriming-associated proteins, and for the first time those which were expressed only in the presence of melatonin. Study confirmed that suitably selected pre-sowing treatment with melatonin, by embryo proteome modification, effectively prepares plants to adverse environmental conditions. In melatonin treated seeds during the initial state of embryos growth, even under optimal conditions, additional antioxidative, detoxifying, anti-stresses proteins were synthesized. Moreover, the supply of energy from seed storage substances was pretty intensified. The presented results partially explain how melatonin acts in plant stress defence, and why plants with higher melatonin content have exhibited increased capacity for stress tolerance.

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Proteomic analysis of heterosis during maize seed germination

Cited by 63 publications

References 64 publications

Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

Relationship among physiological quality, heterosis, and amylase gene expression in maize seeds

Proteomic analysis of muscle between hybrid abalone and parental lines Haliotis gigantea Reeve and Haliotis discus hannai Ino

Exogenous melatonin expediently modifies proteome of maize (Zea mays L.) embryo during seed germination

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