Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride

Leite, Aline L.; Lobo, Janete Gualiume Vaz Madureira; Pereira, Heloísa Aparecida Barbosa da Silva; Fernandes, Marcelo Adorna; Martini, Tatiani; Zucki, Fernanda; Sumida, Dóris Hissako; Rigalli, Alfredo; Buzalaf, Marília Afonso Rabelo

doi:10.1371/journal.pone.0106646

Cited by 50 publications

(40 citation statements)

References 44 publications

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“…Functional enrichment was performed with ClueGo, a Cytoscape plugin. Bioinformatics analysis was performed for each comparison, as described previously (Orchard, ; Bauer‐Mehren, ; Millan, ; Lima Leite et al, ).…”

Section: Methodsmentioning

confidence: 79%

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The effect of fluoride on the structure, function, and proteome of a renal epithelial cell monolayer

Antonio

Jeggle

MacVinish

et al. 2016

Environmental Toxicology

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High concentrations of fluoride in the body may cause toxic effects. Here, we investigated the effects of fluoride on the structure, function, and proteome of a cortical collecting duct epithelium in vitro. Kidney tubule cells (M-1) were chosen because the concentration of fluoride in the kidney is 4-5-fold higher than that in plasma. Mouse M-1 cell monolayers were incubated in fluoride-containing media, and the amiloride-sensitive short-circuit current and transepithelial resistance were measured. The Young's modulus of the epithelium was determined using atomic force microscopy, and the effect of fluoride on epithelial structure was assessed using scanning and transmission electron microscopy, and immunofluorescence. Differences in the expression of membrane proteins were evaluated using proteomics and bioinformatics. Fluoride exposure reduced both transepithelial Na transport and resistance. The IC for fluoride was ∼300 µM for both effects, and the half-times for the decays of ion transport and resistance were 8.4 h and 3.6 days, respectively. Fluoride treatment did not affect the sensitivity of Na transport to amiloride. The Young's modulus of the epithelium was also unaffected by fluoride; however, the functional effects of fluoride were accompanied by marked structural effects. Proteomic analysis revealed changes in expression of a number of proteins, and particularly mitochondrial proteins. Treatment with fluoride had profound effects on the structure, function and proteome of a model cortical collecting duct epithelium. Significantly, however, these effects were produced only at concentrations considerably higher than those likely to be encountered in vivo. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 1455-1467, 2017.

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Section: Methodsmentioning

confidence: 79%

“…Identification of peptides was performed on a nanoAcquity UPLC‐XEVO QToF mass spectometry system (Waters), as previously described (Lima Leite et al, ). Difference in expression between groups was determined using PLGS software and expressed as P < 0.05 for down‐regulated and P > 0.95 for up‐regulated proteins.…”

Section: Methodsmentioning

confidence: 99%

The effect of fluoride on the structure, function, and proteome of a renal epithelial cell monolayer

Antonio

Jeggle

MacVinish

et al. 2016

Environmental Toxicology

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“…The fraction containing hydrophobic proteins was collected and the proteins were purified using detergent removal spin columns (Thermo Scientific). Proteins were extracted, reduced, alkylated, and digested as previously described, and then analyzed using a nanoAcquity UPLC‐XEVO QToF mass spectrometry system . Differences in expression between the groups were assessed using PLGS software and expressed as P < .05 for down‐regulated and (1 – P ) > 0.05 for up‐regulated proteins.…”

Section: Methodsmentioning

confidence: 99%

“…Functional enrichment was analyzed using ClueGo, a Cytoscape plugin. For each comparison, bioinformatics analysis was performed, as described previously …”

Section: Methodsmentioning

confidence: 99%

The effect of fluoride on the structure, function, and proteome of intestinal epithelia

Oliveira

MacVinish

Amin

et al. 2017

Environmental Toxicology

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Fluoride exposure is widespread, with drinking water commonly containing natural and artificially added sources of the ion. Ingested fluoride undergoes absorption across the gastric and intestinal epithelia. Previous studies have reported adverse gastrointestinal effects with high levels of fluoride exposure. Here, we examined the effects of fluoride on the transepithelial ion transport and resistance of three intestinal epithelia. We used the Caco-2 cell line as a model of human intestinal epithelium, and rat and mouse colonic epithelia for purposes of comparison. Fluoride caused a concentration-dependent decline in forskolin-induced Cl -secretion and transepithelial resistance of Caco-2 cell monolayers, with an IC 50 for fluoride of about 3 mM for both parameters. In the presence of 5 mM fluoride, transepithelial resistance fell exponentially with time, with a t 1/2 of about 7 hours. Subsequent imaging by immunofluorescence and scanning electron microscopy showed structural abnormalities in Caco-2 cell monolayers exposed to fluoride. The Young's modulus of the epithelium was not affected by fluoride, although proteomic analysis revealed changes in expression of a number of proteins, particularly those involved in cell-cell adhesion. In line with its effects on Caco-2 cell monolayers, fluoride, at 5 mM, also had profound effects on Cl -secretion and transepithelial resistance of both rat and mouse colonic epithelia. Our results show that treatment with fluoride has major effects on the structure, function, and proteome of intestinal epithelia, but only at concentrations considerably higher than those likely to be encountered in vivo, when much lower fluoride doses are normally ingested on a chronic basis.

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“…Among these are cell cycle, ion transport, apoptosis, signaling pathways and metabolism of free radicals. These effects have been described in several organs and tissues such as liver, kidney, heart, brain, muscle and intestine (Whitford, 1996;Chlubek et al, 2003;Shanthakumari et al, 2004;Strunecka, 2007;Strunecka et al, 2007;Xiong et al, 2007;Blaszczyk et al, 2008;Chouhan e Flora, 2008;Kobayashi et al, 2009;Barbier et al, 2010;Inkielewicz-Stepniak e Czarnowski, 2010;Dey et al, 2011;Pereira et al, 2013;Lima Leite et al, 2014;Pereira et al, 2016;Melo et al, 2017;Dionizio, A. et al, 2018;Dionizio, A. S. et al, 2018;Malvezzi et al, 2018;Pereira et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of mitochondria from rat liver exposed to chronic fluoride exposure in two experimental periods

Araújo¹

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Agradeço a Deus por permitir que mais uma etapa possa ser concluída com muita dignidade, paz de espírito e sabedoria. Obrigada por permitir que eu passasse com muita saúde por todos estes momentos até hoje, sou grata pela vida que tenho, pela família, casa, meus animais, namorado, amigos e profissão. Sou grata senhor por me conceder tamanha sabedoria, paciência e força necessária para acordar todos os dias feliz e em paz para tentar fazer um dia melhor para mim e para as pessoas que estão ao meu redor, gratidão. Aos meus pais que nunca mediram esforços para me criar com muita dignidade, caráter e humildade, obrigada por terem se sacrificado tanto pagando meus estudos, comprando meus materiais, por pagarem meu transporte. Obrigado por me darem a oportunidade de crescer em um lar cheio de paz, amor, respeito, perseverança e persistência. Com isso levo para minha vida como é que uma família deve ser mediante as alegrias e as dificuldades, cheia de amor, respeito, gratidão e com muitos animais ne?!! Hoje estamos aqui, formando a primeira mestre da família, e quem diria que seria a filha de vocês não é mesmo?! Mas como sempre digo, Deus sabe de todas as coisas em nossas vidas e seu propósito é grande. Saibam que serei eternamente grata a vocês, dedico essa e todas as fases da minha vida á vocês. Muito obrigada, amo vocês. Ao meu namorado Junior Você sabe o quão Deus tem sido bom com a gente né?! Como eu poderia imaginar que aquele rapaz andando de moto na rua que eu mexi, hoje viraria parte de tanta coisa na minha vida. Você me completa, me acrescenta e me impulsiona todos os dias. Você é uma das pessoas mais pacientes que eu já conheci, uma das pessoas mais família, que se importa com o bemestar de todos ao seu redor. Você é uma pessoa muito iluminada, que eu tive a sorte de poder conhecer e ter você todos os dias na minha vida. Seu coração genuíno te faz um grande homem. Obrigada por sempre me oferecer uma palavra de conforto ou de incentivo todas as vezes que eu necessitava, obrigada pelo amor puro e sincero, companheirismo, respeito e paciência, você foi essencial para a conclusão desta fase, Te amo.

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Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride

Cited by 50 publications

References 44 publications

The effect of fluoride on the structure, function, and proteome of a renal epithelial cell monolayer

The effect of fluoride on the structure, function, and proteome of a renal epithelial cell monolayer

The effect of fluoride on the structure, function, and proteome of intestinal epithelia

Proteomic analysis of mitochondria from rat liver exposed to chronic fluoride exposure in two experimental periods

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