Proteomic analysis of flowers at two developmental stages in Thermopsis turcica (Fabaceae)

Abstract

IntroductionPlant development is controlled by the interplay of multiple environmental cues and endogenous signals.High-throughput approaches such as proteomics, transcriptomics, and metabolomics have provided immense amounts of data, leading to a better understanding of biological processes (Hochholdinger et al., 2006). As the relationship between mRNA expression levels and protein abundance is poor, proteomics provides functional gene expression profiles during biological processes (Komatsu and Hossain, 2013). Flower development is controlled by a combination of genetics and environmental factors such as light and temperature affecting hormone levels (Davis, 2009;Domagalska et al., 2010). Studies in Arabidopsis thaliana and other angiosperm species including Antirrhinum majus, Petunia hybrid, and Oryza sativa have helped to elucidate the complex network regulated tightly during flower development (Ó'Maoiléidigh et al., 2014). Based on similarity searches, most of the flowering genes were found to be conserved in some legume species (Hecht et al., 2005). Transcription factors play a crucial role in flower development. The regulatory role of MADS-box transcription factors in regulating floral organ development is well demonstrated in soybean and chickpea (Jung et al., 2012;Singh et al., 2013). To date, several proteomic analyses have been employed to identify proteins involved in plant development. However, only a few proteomics studies on flower development are available (Dafny-Yelin et al., 2005;Ahsan and Komatsu, 2009). Analysis of rose petal proteomes showed that energy, cell rescue, and metabolism-related proteins were differentially regulated during development (Dafny-Yelin et al., 2005). The differential expression of several proteins involved in several biological processes such as energy metabolism, secondary metabolism, and signal transduction pathways provides a good example of a protein networks during flower development of soybean (Ahsan and Komatsu, 2009). Zhang et al. (2013) showed the differential expression of several proteins involved in metabolism, protein fate, signal transduction, cellular transport, and biogenesis during floral initiation in Agapanthus praecox. Recently, Zhang et al. (2016) demonstrated that the largest proportions of identified proteins were involved in metabolism, protein fate, and cell rescue during the flowering transition in Crocus sativus.Fabaceae is a large family comprising three subfamilies including Caesalpinioideae, Mimosoideae,

Characterization of rbcL and trnL Plastid DNA Sequences of Vuralia turcica (Fabaceae; Papilionoideae)

“…Previous work has reported that the dominant bacteria at the rhizomes of V. turcica is Bacillus megaterium (Çiftçi, 2018) and the researcher assumed that B. megaterium has the potential growth-promoting effects on V. turcica. Yildiz et al (2017) studied the proteomic analysis in V. turcica and proteins involved in flower development were identified. There has been only one study existed on the genetic classification of V. turcica, and it has been based on the nucleotide sequence of the nuclear ribosomal internal transcribed spacer region (ITS) (Uysal et al, 2014).…”