Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules

Ciregia, Federica; Giusti, Laura; Molinaro, Angelo; Niccolai, Filippo; Mazzoni, Maria Rosa; Rago, Teresa; Tonacchera, Massimo; Vitti, Paolo; Giannaccini, Gino; Lucacchini, Antonio

doi:10.1016/j.trsl.2016.04.004

Cited by 27 publications

(25 citation statements)

References 48 publications

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“…ANXA1 has been found to regulate an EMT-like phenotypic switch and promote metastasis (Graauw & Thompson 2010). Equally important, it has been reported that ANXA1 may help to distinguish benign vs malignant thyroid nodules (Ciregia et al 2016). Our data provide evidence that ANXA1 represents a potential biomarker of thyroid cancer.…”

Section: Spes In Ptc Patients With Lnm Play Critical Roles In Epithelsupporting

confidence: 52%

Proteomics study of serum exosomes from papillary thyroid cancer patients

Luo¹,

Zhan²,

Xia³

et al. 2018

Endocrine-Related Cancer

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Lymph node metastasis (LNM) in papillary thyroid cancer (PTC) is related to increased risk of recurrence and poor prognosis. Tumour exosomes have been shown to be associated with metastasis of cancer cells. Therefore, we aim to identify the characteristics and biological functions of serum exosomes in lymph node metastases of PTC. We compared proteome profiles of serum-purified exosomes (SPEs) from PTC patients with LNM, PTC patients without LNM, and healthy donors, using a combination of liquid chromatography-tandem mass spectroscopy analyses and tandem mass tag label quantitation analysis. We identified 1569 proteins by two or more unique peptides. Compared with the SPEs of PTC patients without LNM, we found 697 differentially expressed proteins in the SPEs of PTC patients with LNM. Our results revealed overexpression of specific proteins with well-established links to cancer cell metastasis, such as SRC, TLN1, ITGB2 and CAPNS1. Consistent with mass spectrum results, we performed Western blot to detect the expression of these proteins in individual sample. Biological pathway analyses showed that integrin signalling was aberrantly activated in the SPEs of PTC patients with LNM compared to those without LNM. Our study reveals that SPEs of PTC patients with lymph node metastases promote BHT101 thyroid cancer cell invasiveness, but have no apparent influence on cell migration. In the serum exosomes of PTC patients with LNM, integrin-associated proteins are obviously upregulated. These proteomic findings will contribute to elucidation of the pathophysiological functions of tumour-derived exosomes.

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Section: Spes In Ptc Patients With Lnm Play Critical Roles In Epithelsupporting

confidence: 52%

Proteomics study of serum exosomes from papillary thyroid cancer patients

Luo¹,

Zhan²,

Xia³

et al. 2018

Endocrine-Related Cancer

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“…Additionally, PARK7 is expressed in 100%, 89.5%, 92.3%, and 88.9% of patients with papillary, follicular, medullary, and anaplastic thyroid cancer, respectively [32]. PARK7 is highly expressed in patients with malignant thyroid cancer, indicating that PARK7 may be involved in thyroid cancer [42]. Furthermore, PARK7 acts as a regulatory subunit of an RNA binding protein and is upregulated in 86% of patients with prostate cancer and closely correlated with reduced survival of prostate cancer patients [43] and in prostate cancer cells [43].…”

Section: Role Of Park7 In Cancer Progressionmentioning

confidence: 99%

Novel Insights into PARK7 (DJ-1), a Potential Anti-Cancer Therapeutic Target, and Implications for Cancer Progression

Jin

2020

JCM

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The expression of PARK7 is upregulated in various types of cancer, suggesting its potential role as a critical regulator of the pathogenesis of cancer and in the treatment of cancer and neurodegenerative diseases, including Parkinson’s disease, Alzheimer’s disease, and Huntington disease. PARK7 activates various intracellular signaling pathways that have been implicated in the induction of tumor progression, which subsequently enhances tumor initiation, continued proliferation, metastasis, recurrence, and resistance to chemotherapy. Additionally, secreted PARK7 has been identified as a high-risk factor for the pathogenesis and survival of various cancers. This review summarizes the current understanding of the correlation between the expression of PARK7 and tumor progression.

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“…CRNN was initially discovered in esophageal cells (Xu et al, 2000). Subsequent studies have shown that CRNN is also expressed in other squamous cells and epithelial cells (Arnouk et al, 2009;Ciregia et al, 2016;Contzler et al, 2005;Imai et al, 2005;Lieden et al, 2009;Salahshourifar et al, 2015;Schrö der et al, 2016;Xiao et al, 2015). It is located primarily in the upper layers of differentiated squamous tissues and plays an important role in epidermal differentiation (Contzler et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Cornulin Is Induced in Psoriasis Lesions and Promotes Keratinocyte Proliferation via Phosphoinositide 3-Kinase/Akt Pathways

Li¹,

Xiao

Jia

et al. 2019

Journal of Investigative Dermatology

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Psoriasis is a chronic inflammatory skin disease characterized by abnormal proliferation of epidermal keratinocytes and infiltration of inflammatory cells. CRNN is a major component of the cornified cell envelope and implicated in several epithelial malignancies. Here, we show that CRNN expression was increased in the lesioned epidermis from the patients with psoriasis vulgaris and skin lesions from the imiquimod (IMQ)-treated mice. Expression of CRNN in cultured keratinocytes (HEKa and HaCaT) was also induced by M5, a mixture of five pro-inflammatory cytokines (i.e., IL-17A, IL-22, IL-1a, oncostatin M, and TNF-a). Lentiviral expression of CRNN increased cell proliferation by inducing cyclin D1. Conversely, knockdown of CRNN by small interfering RNA suppressed G1/S transition and attenuated the M5-induced proliferation. In addition, CRNN overexpression increased the phosphorylation and activation of phosphoinositide 3-kinase and Akt. Inactivation of the phosphoinositide 3-kinase and Akt pathways using small interfering RNA or selective inhibitors (LY294002 and MK2206) reduced the proliferative effects of CRNN. Furthermore, topical use of anti-psoriatic calcipotriol effectively decreased expression of CRNN, inhibited the Akt activation and improved the IMQ-stimulated psoriasis-like pathologies. Taken together, these results suggest that induced expression of CRNN may contribute to the pathogenesis of psoriasis.

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Proteomic analysis of fine-needle aspiration in differential diagnosis of thyroid nodules

Cited by 27 publications

References 48 publications

Proteomics study of serum exosomes from papillary thyroid cancer patients

Proteomics study of serum exosomes from papillary thyroid cancer patients

Novel Insights into PARK7 (DJ-1), a Potential Anti-Cancer Therapeutic Target, and Implications for Cancer Progression

Cornulin Is Induced in Psoriasis Lesions and Promotes Keratinocyte Proliferation via Phosphoinositide 3-Kinase/Akt Pathways

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