Proteomic analysis of Exosomes derived from the Aqueous Humor of Myopia Patients

Tsai, Ching Yao; Chen, Chueh Tan; Lin, Chin Hui; Liao, Chen Chung; Hua, Kate; Hsu, Cheng Hsiung; Chen, Chian Feng

doi:10.7150/ijms.51735

Cited by 16 publications

(7 citation statements)

References 39 publications

(47 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lines of evidence suggest abundant exosomes are common in AH [15,16]. Recent research has linked exosomal proteins or miRNAs to variety of ophthalmic diseases [17][18][19][20]. To examine the changes of exosomal proteins that occur with the development of AMD, we aimed to compare the individual protein profiles from patients with AMD, using liquid chromatography-tandem mass spectrometry (LC-MS/MS).…”

Section: Introductionmentioning

confidence: 99%

Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients

Tsai¹,

Chen²,

Wu³

et al. 2022

Int. J. Med. Sci.

Self Cite

View full text Add to dashboard Cite

Purpose:The alteration of the exosomal proteins in the aqueous humor (AH) is linked to the development of eye diseases. The goal of this study was to examine the exosomal protein profile of patients with age-related macular degeneration (AMD) to better understand their role in the pathogenesis of AMD. Methods: Exosomes were isolated from the AH of 28 AMD and 25 control eyes. The quality, concentration, and size distribution of exosomes were measured using a nanoparticle tracking analysis system (NTA). Total exosomal proteins from each sample were purified and digested with trypsin for liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Results: Based on LC-MS/MS analysis, we got 105 exosomal peptides from AMD and control patients. Gene ontology (GO) analysis in the biology process revealed that exosomal proteins of AMD were enriched in the lipoprotein metabolic process. T-test analysis revealed six exosomal proteins in patients with AMD were significantly different from controls. Comparing the exosomal protein profile of AMD patients who were receiving anti-VEGF therapy, we observed the amount of two proteins decreased with the duration of the anti-VEGF treatment time. Conclusions: In this study, we successfully isolated and purified AH exosomes. Our results provide pioneering findings for the exosomal protein profile in AMD development and under therapy. These unique proteins could be the new targets for drug discovery or biological markers for evaluating therapeutic efficacy.

show abstract

Section: Introductionmentioning

confidence: 99%

Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients

Tsai¹,

Chen²,

Wu³

et al. 2022

Int. J. Med. Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…4 MicroRNAs and proteins in aqueous humor exosomes differentiate between patients with high myopia and non-myopia. 5 , 6 It has been reported that exosomes secreted by mesenchymal stem cells and neural progenitor cells can protect retinal photoreceptors and combat retinal degeneration through a variety of microRNA. 7 , 8 In this way, exosomal microRNAs may play an important role in myopic maculopathy.…”

mentioning

confidence: 99%

Exosomal MicroRNA Profiling in Vitreous Humor Derived From Pathological Myopia Patients

You

et al. 2023

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

Purpose Pathologic myopia (PM) is one of the primary causes of blindness. This study aims to explore the possible relations between the composition of microRNA in vitreous exosomes of patients with PM and the progression of myopic maculopathy. Methods Vitreous humor (VH) samples were collected from patients undergoing retinal surgery. A total of 15 and 12 VH samples were obtained from patients with PM and control, respectively. The PM group was divided into PM-L (G2) and PM-H groups (G3 and G4) in order to explore differentially expressed microRNAs (DEMs) that account for the relatively poor prognosis in G3 and G4 myopic maculopathy. A Weighted Gene Co-Expression Network Analysis (WGCNA) was conducted to find the persistently altered key microRNAs in myopic maculopathy progression. The Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analysis were used. Results High purity exosomes were extracted from the vitreous fluid of patients with PM and control. The top five downregulated DEMs of PM-H versus PM-L can reflect the tendency of deterioration of PM-H myopic maculopathy. MiR-143-3p and miR-145-5p , which were found in WGCNA, may participate in the development of myopic maculopathy. These microRNAs all relate to the insulin resistance pathway. Conclusions This is the first study to explore the relations between the progression of myopic maculopathy and vitreous exosomal microRNAs. Vitreous exosomal miR-143-3p and miR-145-5p can be considered biomarkers for patients with PM, and the vitreous exosomal DEM associated with PM-H may represent alarming signals of myopic maculopathy deterioration.

show abstract

“…Accordingly, we enrolled 3 groups, including the non-MM, MAM, and pmCNV groups, for proteomic testing. Several studies have investigated alterations in AH protein expression in human samples in myopic populations through high-throughput techniques [ 14 , 15 ], but few have strictly classified the different types of MM depending on the practical and newest category—the ATN grading system [ 12 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Insights into myopic choroidal neovascularization based on quantitative proteomics analysis of the aqueous humor

Yu,

Zhong,

Zhao

et al. 2023

BMC Genomics

View full text Add to dashboard Cite

Background Previous studies on the biomarkers of pathologic myopia choroidal neovascularization (pmCNV) development merely detected limited types of proteins and provide a meagre illustration of the underlying pathways. Hence, a landscape of protein changes in the aqueous humor (AH) of pmCNV patients is lacking. Here, to explore the potential mechanisms and biomarkers of pmCNV, we analyzed the clinical data and protein profile among atrophic (A) lesions, tractional lesions (T) and neovascular (N) lesions in myopic patients based on the ATN grading system for myopic maculopathy (MM). Results After investigating demographic data of our patients, a correlation was found between A and N lesions (R = 0.5753, P < 0.0001). Accordingly, groups were divided into patients without MM, patients with myopic atrophic maculopathy (MAM), and patients with pmCNV (N2a lesion). In proteomics analysis, the increased protein level of GFAP and complement-associated molecules in AH samples of the 3 groups also indicated that MAM and pmCNV shared similar characteristics. The GO enrichment and KEGG pathway analysis were performed, which mapped that differential expressed proteins mainly engaged in JAK-STAT pathway between the pmCNV group and two controls. Furthermore, we identified several potential biomarkers for pmCNV, including FCN3, GFAP, EGFR, SFRP3, PPP2R1A, SLIT2, and CD248. Conclusions Atrophic lesions under pathologic myopic conditions demonstrated similarities to neovascularization development. Potential biomarkers including GFAP were associated with the pathogenesis of pmCNV. In summary, our study provides new insights for further research on pmCNV development.

show abstract

Proteomic analysis of Exosomes derived from the Aqueous Humor of Myopia Patients

Cited by 16 publications

References 39 publications

Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients

Proteomic Profiling of Aqueous Humor Exosomes from Age-related Macular Degeneration Patients

Exosomal MicroRNA Profiling in Vitreous Humor Derived From Pathological Myopia Patients

Insights into myopic choroidal neovascularization based on quantitative proteomics analysis of the aqueous humor

Contact Info

Product

Resources

About