Proteomic analysis of exhaled breath condensate for diagnosis of pathologies of the respiratory system

Kononikhin, Alexey; Fedorchenko, Kristina; Ryabokon, Anna; Стародубцева, Н. Л.; Popov, Igor; Zavialova, Maria G.; Анаев, Э. Х.; Чучалин, А. Г.; Варфоломеев, С. Д.; Nikolaev, E. N.

doi:10.18097/pbmc20156106777

Cited by 7 publications

(3 citation statements)

References 15 publications

(24 reference statements)

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“…Finally, 119 studies were selected in this systematic review. Details and related findings of selected papers (age, sex, sample type, type of technique used, statistical analysis, number of increased or decreased proteins, model characteristics, validation test) are shown in supplementary files 20–123 . It listed for COPD, IPF, Asthma, BO, and mustard gas‐exposed victims, respectively.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of common protein biomarkers involved in the pathogenesis of respiratory diseases with proteomic methods: A systematic review

Rezaeeyan,

Arabfard,

Rasouli

et al. 2023

Immunity Inflam & Disease

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AimRespiratory disease (RD) is one of the most common diseases characterized by lung dysfunction. Many diagnostic mechanisms have been used to identify the pathogenic agents of responsible for RD. Among these, proteomics emerges as a valuable diagnostic method for pinpointing the specific proteins involved in RD pathogenesis. Therefore, in this study, for the first time, we examined the protein markers involved in the pathogenesis of chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis (IPF), asthma, bronchiolitis obliterans (BO), and chemical warfare victims exposed to mustard gas, using the proteomics method as a systematic study.Materials and MethodsA systematic search was performed up to September 2023 on several databases, including PubMed, Scopus, ISI Web of Science, and Cochrane. In total, selected 4246 articles were for evaluation according to the criteria. Finally, 119 studies were selected for this systematic review.ResultsA total of 13,806 proteins were identified, 6471 in COPD, 1603 in Asthma, 5638 in IPF, three in BO, and 91 in mustard gas exposed victims. Alterations in the expression of these proteins were observed in the respective diseases. After evaluation, the results showed that 31 proteins were found to be shared among all five diseases.ConclusionAlthough these 31 proteins regulate different factors and molecular pathways in all five diseases, they ultimately lead to the regulation of inflammatory pathways. In other words, the expression of some proteins in COPD and mustard‐exposed patients increases inflammatory reactions, while in IPF, they cause lung fibrosis. Asthma, causes allergic reactions due to T‐cell differentiation toward Th2.

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Section: Resultsmentioning

confidence: 99%

Evaluation of common protein biomarkers involved in the pathogenesis of respiratory diseases with proteomic methods: A systematic review

Rezaeeyan,

Arabfard,

Rasouli

et al. 2023

Immunity Inflam & Disease

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“…In previous studies cytoskeleton keratins were shown as to be main proteins in the EBC of healthy peoples [44,45]. Some of them are also associated with different pathologies of the respiratory system such as pneumonia and lung cancer [37,38,46]. Due to Protein Atlas, keratins KRT1 , KRT 2 , KRT 9 , and KRT 10 have epidermal origin and can contaminate samples during sample preparation.…”

Section: Resultsmentioning

confidence: 99%

Proteome Profiling of the Exhaled Breath Condensate after Long-Term Spaceflights

Kononikhin

Brzhozovskiy

Ryabokon

et al. 2019

IJMS

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Comprehensive studies of the effects of prolonged exposure to space conditions and the overload experienced during landing on physiological and biochemical changes in the human body are extremely important in the context of planning long-distance space flights, which can be associated with constant overloads and various risk factors for significant physiological changes. Exhaled breath condensate (EBC) can be considered as a valuable subject for monitoring physiological changes and is more suitable for long-term storage than traditional monitoring subjects such as blood and urine. Herein, the EBC proteome changes due to the effects of spaceflight factors are analyzed. Thirteen EBC samples were collected from five Russian cosmonauts (i) one month before flight (background), (ii) immediately upon landing modules in the field (R0) after 169–199 days spaceflights, and (iii) on the seventh day after landing (R+7). Semi-quantitative label-free EBC proteomic analysis resulted in 164 proteins, the highest number of which was detected in EBC after landing (R0). Pathways enrichment analysis using the GO database reveals a large group of proteins which take part in keratinization processes (CASP14, DSG1, DSP, JUP, and so on). Nine proteins (including KRT2, KRT9, KRT1, KRT10, KRT14, DCD, KRT6C, KRT6A, and KRT5) were detected in all three groups. A two-sample Welch’s t-test identified a significant change in KRT2 and KRT9 levels after landing. Enrichment analysis using the KEGG database revealed the significant participation of detected proteins in pathogenic E. coli infection (ACTG1, TUBA1C, TUBA4A, TUBB, TUBB8, and YWHAZ), which may indicate microbiota changes associated with being in space. This assumption is confirmed by microbial composition analysis. In general, the results suggest that EBC can be used for noninvasive monitoring of health status and respiratory tract pathologies during spaceflights, and that the obtained data are important for the development of medicine for use in extreme situations. Data are available from ProteomeXchange using the identifier PXD014191.

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“…Another study in this area was carried out by Kononikhin et al who investigated the protein composition of EBC from patients with different respiratory diseases (seventeen COPD patients, thirteen patients with pneumonia and twenty‐three healthy non‐smokers). Three differentially expressed proteins (dermcidin,DCD; α1‐microglobulin/bikunin precursor and SHROOM3) attracted authors’ attention.…”

Section: Biological Fluidsmentioning

confidence: 99%

Searching for biomarkers of chronic obstructive pulmonary disease using proteomics: The current state

et al. 2018

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Detection of proteins which may be potential biomarkers of disorders represents a big step forward in understanding the molecular mechanisms that underlie pathological processes. In this context proteomics plays the important role of opening a path for the identification of molecular signatures that can potentially assist in early diagnosis of several clinical disturbances. Aim of this report is to provide an overview of the wide variety of proteomic strategies that have been applied to the investigation of chronic obstructive pulmonary disease (COPD), a severe disorder that causes an irreversible damage to the lungs and for which there is no cure yet. The results in this area published over the past decade show that proteomics indeed has the ability of monitoring alterations in expression profiles of proteins from fluids/tissues of patients affected by COPD and healthy controls. However, these data also suggest that proteomics, while being an attractive tool for the identification of novel pathological mediators of COPD, remains a technique mainly generated and developed in research laboratories. Great efforts dedicated to the validation of these biological signatures will result in the proof of their clinical utility.

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Proteomic analysis of exhaled breath condensate for diagnosis of pathologies of the respiratory system

Cited by 7 publications

References 15 publications

Evaluation of common protein biomarkers involved in the pathogenesis of respiratory diseases with proteomic methods: A systematic review

Evaluation of common protein biomarkers involved in the pathogenesis of respiratory diseases with proteomic methods: A systematic review

Proteome Profiling of the Exhaled Breath Condensate after Long-Term Spaceflights

Searching for biomarkers of chronic obstructive pulmonary disease using proteomics: The current state

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