Proteomic analysis of differential protein expression in neuropathic pain and electroacupuncture treatment models

Sung, Ho Joong; Kim, Yoonn Suk; Kim, In Sik; Jang, Su-Jin; Kim, Yoon Rim; Na, Doe Sun; Han, Ki Hoon; Hwang, Byung Gil; Park, Dong Suk; Ko, Jesang

doi:10.1002/pmic.200300821

Cited by 42 publications

(37 citation statements)

References 36 publications

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“…In a rat model of neuropathic pain, the Ca-binding proteins calmodulin and calretinin as well as protein-tyrosin phosphatase A, Ras-related protein RAB-15, and stress-activated protein kinase JNK3 showed lower levels. Higher levels were found for DRP2, interleukin-1, Rab-3A and 1B, somatropin, myosin regulatory light chain, and neuronalkinesin heavy chain [69,70]. In an ischemia model, increased expression for the signaling molecules platelet activation factor receptor and retinal guanyl cyclase 2 were observed [68].…”

Section: Signal Transduction Proteinsmentioning

confidence: 85%

“…In contrast to DS, down-regulation for ubiquitin carboxylterminal esterase L-1 was found in the brain of patients with schizophrenia [66]. In a rat model for neuropathic pain, reduced levels for proteasome component C8 were observed [69].…”

Section: Protein Metabolismmentioning

confidence: 93%

“…Increased levels for fructosebiphosphate aldolase were also observed in bipolar disorder [55]. In an animal model of neuropathic pain, several proteins involved in metabolic pathways were found to have altered levels, alpha enolase, cell division protein kinase 4, delayed recitifier K 1 channel (all increased), nitric oxide synthase 3, phosphorylase B kinase catalytic chain, porphobilinogen deaminase, and ubiquinone biosynthesis protein (decreased) [69,70]. In a rat model of hypoxia, the levels of NADH dehydrogenase Fe-S protein decreased [127] and in a model of stroke, propionyl CoA carboxylase and vasolin-containing protein showed altered phosphorylation patterns [128].…”

Section: Energy Metabolism Proteinsmentioning

confidence: 94%

“…Decreased levels were found for caspase-3, -8, and -9 in the AD and DS brain [47,95,98], as well as for the DNA fragmentation factor 45 (DFF45) in AD, a caspase-activated heat shock protein [98]. Caspase-1 showed decreased levels in a rat model of neuropathic pain [69]. The reduced caspase levels indicate that apoptosis is a mechanism of cell death in AD and DS and that caspases actively contribute to the molecular pathogenesis of these diseases and support the development of caspase inhibitors as potential therapeutic approaches for chronic neurodegenerative disorders [97].…”

Section: Apoptosis-related Proteinsmentioning

confidence: 99%

“…Neuropathic pain, in contrast to nociceptive pain, is described as "burning", "electric", "tingling", and "shooting" in nature [69,70]. Limited proteomic analysis of neuropathic pain exists so far.…”

Section: Neuropathic Painmentioning

confidence: 99%

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Proteomics‐driven progress in neurodegeneration research

Fountoulakis

Κοσσίδα

2006

Electrophoresis

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Proteomics-driven progress in neurodegeneration researchProteomics technologies have been widely used in the investigation of neurodegenerative and psychiatric disorders, and in particular in the detection of differences between healthy individuals and patients suffering from such diseases. Thus, brain and cerebrospinal fluid (CSF) samples from patients with Alzheimer's disease, Down syndrome, Pick's disease, Parkinson's disease, schizophrenia, and other disorders as well as brain and CSF from animals serving as models of neurological disorders have been analyzed by proteomics. 2-DE followed by MALDI-TOF-MS has been mainly applied as this proteomics approach provides the possibility of convenient quantification of protein levels and detection of post-translational modifications. About 330 unique proteins with deranged levels and modifications have been detected by proteomics approaches to be related to neurodegeneration and psychiatric disorders. They are mainly involved in metabolism pathways, cytoskeleton formation, signal transduction, guidance, detoxification, transport, and conformational changes. In this article, we provide a summary of the major contributions of proteomics technologies in the study of neurodegenerative and psychiatric diseases, in particular, in the detection of changes in protein levels and modifications related to these disorders. IntroductionNeurodegeneration is a major hallmark of a series of genetic and acquired disorders of the central nervous system (CNS), like Alzheimer's disease (AD), Down syndrome (DS), Pick's disease, Creutzfeldt-Jakob disease (CJD), and Parkinson's disease, which result in severe cognitive and motor deficits. A common characteristic of these diseases is that they are multifactorial.Their molecular basis is unknown and individual biomarkers are not reliable. Genomics and proteomics approaches have been applied to obtain a better understanding of neurodegeneration and psychiatric disorders. Genomics has been used to map and identify genes that are mutated in a wide variety of such disorders, like presenilins in AD. Next to genomics, which only provides a partial view of the biological problem, proteomics is widely used in clinical diagnosis as well and many changes in protein levels associated with specific diseases have been identified [1,2].Proteomics is a technology-driven science that studies the proteins of the various biological systems, their structures, interactions, post-translational modifications, and in particular the changes in their levels and modifications, which are the result of specific diseases or are induced by external factors.

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Section: Signal Transduction Proteinsmentioning

confidence: 85%

Section: Protein Metabolismmentioning

confidence: 93%

Section: Energy Metabolism Proteinsmentioning

confidence: 94%

Section: Apoptosis-related Proteinsmentioning

confidence: 99%

Section: Neuropathic Painmentioning

confidence: 99%

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Proteomics‐driven progress in neurodegeneration research

Fountoulakis

Κοσσίδα

2006

Electrophoresis

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Current awareness on comparative and functional genomics

2005

Comp Funct Genom

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Weed genomics: New tools to understand weed biology (Review). Trends Plant Sci 9: (8) 391. Baxevanis AD. 2003. Using genomic databases for sequence-based biological discovery (Review). Mol Med 9: (9-12) 185. Benning C. 2004. Genetic mutant screening by direct metabolite analysis (Review). Anal Biochem 332: (1) 1. Brouzes E, Farge E. 2004. Interplay of mechanical deformation and patterned gene expression in developing embryos. Curr Opin Genet Dev 14: (4) 367. Brunner AM, Nilsson O. 2004. Revisiting tree maturation and floral initiation in the poplar functional genomics era (Review). New Phytol 164: (1) 43.

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Pigment epithelium-derived factor is differentially expressed in peripheral neuropathies

Conti¹,

Ricchiuto²,

Iannaccone³

et al. 2005

Proteomics

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Peripheral neuropathies are characterized by asymmetrical slowly progressive weakness with no upper motor neuron signs, and can occur either with or without pain. Due to poor knowledge of the disease mechanisms, available pain treatment is very limited. Because of the difficulties and invasiveness involved when performing direct analysis on peripheral and CNS, pathological markers can be searched for in the cerebrospinal fluid (CSF) as an alternative. To investigate pain mechanisms in peripheral neuropathy and find diagnostic markers, CSF samples were analyzed by a differential expression proteomic approach. We studied CSF from: neuropathic patients with pain (PN), without pain (NPN) and healthy controls (CN). 2-DE analysis showed ten protein spots differentially expressed, and six of these were identified by MS. In NPN patients we found an expression level decrease of three pigment epithelium-derived factor (PEDF) protein isoforms. Immunoblot with a specific antibody revealed the presence of additional PEDF isoforms not highlighted by differential expression analysis. Fucose residues on the oligosaccharide chain were found only in the isoforms down regulated in NPN patients. Considered as PEDF has important neurobiological effects, it might be considered an interesting pathology marker.

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Proteomic analysis of differential protein expression in neuropathic pain and electroacupuncture treatment models

Cited by 42 publications

References 36 publications

Proteomics‐driven progress in neurodegeneration research

Proteomics‐driven progress in neurodegeneration research

Current awareness on comparative and functional genomics

Pigment epithelium-derived factor is differentially expressed in peripheral neuropathies

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