Proteomic analysis of core breakdown disorder in Conference pears (Pyrus communisL.)

Abstract

2-DE was applied to study core breakdown disorder in controlled atmosphere stored 'Conference' pears. This physiological disorder is characterized by internal browning of the fruit tissue and the development of cavities. Suitable protein phenol extraction/ammonium acetate-methanol precipitation and 2-DE protocols for a wide pH range were established for pear tissue. The protein expression profiles of healthy, sound (intact tissue of pears with core breakdown) and brown tissue were analyzed with the univariate non-parametric Kolmogorov-Smirnov test and multivariate statistical techniques such as principal component analysis and partial least square discriminant analysis. Both statistical approaches revealed interesting differentially expressed proteins between healthy and disordered pears. LC-ESI-MS/MS identification of differentially expressed proteins between healthy and sound tissue revealed their participation in the energy metabolism, the antioxidant system and ethylene biosynthesis. Up-regulated characteristic proteins in brown tissue were mainly involved in energy metabolism and defense mechanisms. Proteomics coupled to univariate and multivariate statistical techniques seems to be an efficient approach to get a better insight into the different mechanisms and pathways leading to the core breakdown disorder.

Transcriptome and metabolome analysis of Citrus fruit to elucidate puffing disorder

“…TCA cycle genes such as fumarase and succinyl-CoA ligase were upregulated. These findings are consistent with studies showing that fumarase was upregulated in fruit tissues affected by core breakdown disorder in pear [30]. Further experiments at the proteomic and enzymatic levels may be necessary to determine whether this perturbation in glycolysis associated with puffing disorder decreases production of ATP, the reduced form of nicotinamide adenine dinucleotide (NADH), or other high energy cofactors.…”

Optimizing protein extraction from plant tissues for enhanced proteomics analysis

“…Moreover, protein content in fruits (e. g., grape and apple) is relatively low. Recently, protocols have been developed to make successful extraction of proteins from grape, olive, apple, banana, strawberry, and pear among others [34,37,39,54].…”

Insights into fruit function from the proteome of the hypanthium

“…This makes proteomics an important tool in systems biology, as it allows the global analysis of a subset of gene products expressed and/or co-expressed in a tissue or cell at a specific physiological state (Blackstock and Weir, 1999). The use of proteomic approaches in the area of vegetable and fruit physiology, including tomato (Lycopersicon esculentum; Rocco et al, 2008), strawberry (Fragaria ananassa; Hjernø et al, 2006), pear (Pedreschia et al, 2007(Pedreschia et al, , 2009; Thomas et al, 2010) and apple (Guarino et al, 2007;Herndl et al, 2007;Cao et al, 2008;Marzban et al, 2008;Maghuly et al, 2009;Qin et al, 2009) has recently emerged. They revealed the importance of proteomics to qualitatively and quantitatively infer regulatory events occurring during plant and fruit development.…”