Proteomic analysis of Chilo iridescent virus

İnce, İkbal Agah; Boeren, Sjef; Oers, Monique M. van; Vervoort, Jacques; Vlak, Just M.

doi:10.1016/j.virol.2010.05.038

Cited by 42 publications

(30 citation statements)

References 42 publications

(60 reference statements)

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“…Surprisingly, at odd with icosahedral DNA viruses32333536434445464748, the major capsid protein was not found to be the most abundant in the virion proteomes. Instead, it is a 150 residues long protein of unknown function, specific and highly conserved in all Marseilleviridae (>77% identity).…”

Section: Discussionmentioning

confidence: 96%

Noumeavirus replication relies on a transient remote control of the host nucleus

et al. 2017

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Acanthamoeba are infected by a remarkable diversity of large dsDNA viruses, the infectious cycles of which have been characterized using genomics, transcriptomics and electron microscopy. Given their gene content and the persistence of the host nucleus throughout their infectious cycle, the Marseilleviridae were initially assumed to fully replicate in the cytoplasm. Unexpectedly, we find that their virions do not incorporate the virus-encoded transcription machinery, making their replication nucleus-dependent. However, instead of delivering their DNA to the nucleus, the Marseilleviridae initiate their replication by transiently recruiting the nuclear transcription machinery to their cytoplasmic viral factory. The nucleus recovers its integrity after becoming leaky at an early stage. This work highlights the importance of virion proteomic analyses to complement genome sequencing in the elucidation of the replication scheme and evolution of large dsDNA viruses.

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Section: Discussionmentioning

confidence: 96%

Noumeavirus replication relies on a transient remote control of the host nucleus

et al. 2017

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“…IIV-6 was kindly supplied by C. Joel Funk (US Department of Agriculture-Agricultural Research Service Western Cotton Research Laboratory, Phoenix, AZ) and was propagated in Galleria mellonella (greater wax moth) larvae and purified by 25-65% (wt/vol) sucrose density gradient centrifugation as described previously (70,71). IIV-6 UV-inactivated virus was produced by exposing the virus stock to a total of 12,000 mJ of UV light in four intervals of 3 min (72).…”

Section: Methodsmentioning

confidence: 99%

The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery

Bronkhorst

Cleef²,

Vodovar³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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132

155

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RNA viruses in insects are targets of an RNA interference (RNAi)-based antiviral immune response, in which viral replication intermediates or viral dsRNA genomes are processed by Dicer-2 (Dcr-2) into viral small interfering RNAs (vsiRNAs). Whether dsDNA virus infections are controlled by the RNAi pathway remains to be determined. Here, we analyzed the role of RNAi in DNA virus infection using Drosophila melanogaster infected with Invertebrate iridescent virus 6 (IIV-6) as a model. We show that Dcr-2 and Argonaute-2 mutant flies are more sensitive to virus infection, suggesting that vsiRNAs contribute to the control of DNA virus infection. Indeed, small RNA sequencing of IIV-6-infected WT and RNAi mutant flies identified abundant vsiRNAs that were produced in a Dcr-2-dependent manner. We observed a highly uneven distribution with strong clustering of vsiRNAs to small defined regions (hotspots) and modest coverage at other regions (coldspots). vsiRNAs mapped in similar proportions to both strands of the viral genome, suggesting that long dsRNA derived from convergent overlapping transcripts serves as a substrate for Dcr-2. In agreement, strand-specific RT-PCR and Northern blot analyses indicated that antisense transcripts are produced during infection. Moreover, we show that vsiRNAs are functional in silencing reporter constructs carrying fragments of the IIV-6 genome. Together, our data indicate that RNAi provides antiviral defense against dsDNA viruses in animals. Thus, RNAi is the predominant antiviral defense mechanism in insects that provides protection against all major classes of viruses.insect immunity | Iridoviridae | siRNA | antiviral immunity | small RNA profiling | next-generation sequencing

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“…In-gel protein digestions and peptide extractions were performed at 25 uC according to a method described previously (Ince et al, 2010). The peptides resulting from this digestion were analysed by LC-MS/ MS by injecting 18 ml sample onto a 0.10632 mm Prontosil 300-5-C18H pre-concentration column (Bischoff) at a flow rate of 6 ml min 21 for 5 min.…”

Section: Methods Preparation Of Virus Particles and Analysis By Lc-msmentioning

confidence: 99%

Proteomic analysis of Glossina pallidipes salivary gland hypertrophy virus virions for immune intervention in tsetse fly colonies

Kariithi

İnce

Boeren

et al. 2010

Journal of General Virology

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Many species of tsetse flies (Diptera: Glossinidae) can be infected by a virus that causes salivary gland hypertrophy (SGH). The genomes of viruses isolated from Glossina pallidipes (GpSGHV) and Musca domestica (MdSGHV) have recently been sequenced. Tsetse flies with SGH have reduced fecundity and fertility which cause a serious problem for mass rearing in the frame of sterile insect technique (SIT) programmes to control and eradicate tsetse populations in the wild. A potential intervention strategy to mitigate viral infections in fly colonies is neutralizing of the GpSGHV infection with specific antibodies against virion proteins. Two major GpSGHV virion proteins of about 130 and 50 kDa, respectively, were identified by Western analysis using a polyclonal rabbit antibody raised against whole GpSHGV virions. The proteome of GpSGHV, containing the antigens responsible for the immune-response, was investigated by liquid chromatography tandem mass spectrometry and 61 virion proteins were identified by comparison with the genome sequence. Specific antibodies were produced in rabbits against seven candidate proteins, including the ORF10/C-terminal fragment, ORF47 and ORF96 as well as proteins involved in peroral infectivity PIF-1 (ORF102), PIF-2 (ORF53), PIF-3 (ORF76) and P74 (ORF1). Antiserum against ORF10 specifically reacted to the 130 kDa protein in a Western blot analysis and to the envelope protein of GpSGHV, detected by using immunogold-electron microscopy. This result suggests that immune intervention of viral infections in colonies of G. pallidipes is a realistic option.

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Proteomic analysis of Chilo iridescent virus

Cited by 42 publications

References 42 publications

Noumeavirus replication relies on a transient remote control of the host nucleus

Noumeavirus replication relies on a transient remote control of the host nucleus

The DNA virus Invertebrate iridescent virus 6 is a target of the Drosophila RNAi machinery

Proteomic analysis of Glossina pallidipes salivary gland hypertrophy virus virions for immune intervention in tsetse fly colonies

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