Proteomic analysis of chicken embryonic trachea and kidney tissues after infection in ovo by avian infectious bronchitis coronavirus

Abstract

BackgroundAvian infectious bronchitis (IB) is one of the most serious diseases of economic importance in chickens; it is caused by the avian infectious coronavirus (IBV). Information remains limited about the comparative protein expression profiles of chicken embryonic tissues in response to IBV infection in ovo. In this study, we analyzed the changes of protein expression in trachea and kidney tissues from chicken embryos, following IBV infection in ovo, using two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF-TOF MS).Results17 differentially expressed proteins from tracheal tissues and 19 differentially expressed proteins from kidney tissues were identified. These proteins mostly related to the cytoskeleton, binding of calcium ions, the stress response, anti-oxidative, and macromolecular metabolism. Some of these altered proteins were confirmed further at the mRNA level using real-time RT-PCR. Moreover, western blotting analysis further confirmed the changes of annexin A5 and HSPB1 during IBV infection.ConclusionsTo the best of our knowledge, we have performed the first analysis of the proteomic changes in chicken embryonic trachea and kidney tissues during IBV infection in ovo. The data obtained should facilitate a better understanding of the pathogenesis of IBV infection.

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“…Cao and co-workers used the same approach to analyze the differentially expressed proteins after an IBV infection (Cao et al, 2011(Cao et al, , 2012. They analyzed chicken embryonic trachea and kidney tissues after in ovo infection by IBV using 2-DE with Coomassie Blue staining and MALDI-TOF-TOF/MS.…”

“…They analyzed chicken embryonic trachea and kidney tissues after in ovo infection by IBV using 2-DE with Coomassie Blue staining and MALDI-TOF-TOF/MS. Here, they found 17 differentially expressed proteins from tracheal tissue and 19 from kidney tissue (Cao et al, 2011). In their subsequent report, they analyzed the differentially expressed proteins in trachea and kidney tissues from chicken at different stages infected in vivo with two different IBV strains -a highly virulent and an attenuated one (Cao et al, 2012).…”

Isolamento e propagação de Astrovírus, Adenovírus, Coronavírus, Parvovírus, Rotavírus e Reovírus de aves comerciais com problemas entéricos em ovos embrionados

“…Foi caracterizado em pombos e patos mortos ou doentes, mostrando similaridade significativa com cepas do ANV, comprovando que o vírus está se disseminando em mais espécies de aves (BIDIN et al, 2011;SHAO et al, 2011).…”

“…A idade embrionária é considerada determinante no sucesso ou fracasso do isolamento viral, idades padrão são consideradas no momento da inoculação, sugerindo de 9 a 11 dias para a inoculação por via saco alantóide, 10 a 11 dias para a inoculação por via membrana corioalantóide, 10 a 11 dias para a infecção por via saco amniótico e de 6 a 8 dias para a inoculação por via saco da gema (SENNE, 1998 Uma marcada presença de hemorragias, edema, nanismo, deformidades e aspecto gelatinoso foram as lesões macroscópicas encontradas nos embriões, e estas correspondem à descrições anteriores IMADA, 2008;SCHULTZ-CHERRY, 2008;ALAMNESH et al, 2010;CAO et al, 2011).…”

“…As características macroscópicas apresentadas nos embriões onde se isolou o vírus da bronquite infecciosa das galinhas foram as mesmas já descritas com isolados provenientes de aves com problemas respiratórios e inoculadas por via saco alantóide (CABANAGH; GELB, 2008;CAO et al, 2011), Neste estudo, nas três primeiras passagens os embriões se apresentavam normais e a partir da terceira passagem ocorreu nanismo, hemorragias e com frequência, o enrolamento .…”