Proteomic analysis of changes in protein expression in liver mitochondria in apoE knockout mice

Suski, Maciej; Olszanecki, Rafał; Madej, Józef; Totoń‐Żurańska, Justyna; Niepsuj, Anna; Jawień, Jacek; Bujak-Giżycka, Beata; Okoń, Krzysztof; Korbut, Ryszard

doi:10.1016/j.jprot.2011.03.003

Cited by 18 publications

(13 citation statements)

References 30 publications

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“…This result indicates that the detection of MUP1 in isolated mitochondria was not a result of contact between mitochondria and ER. Indeed, similar to our results, MUP1 was also found in isolated liver mitochondria via immunoblotting in another study . Besides, MUP1 has been reported to enhance mitochondrial biogenesis .…”

Section: Discussionsupporting

confidence: 92%

Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures

Zhou

Liu

et al. 2019

FEBS Letters

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Laboratory mice are standardly housed at around 23 °C, setting them under chronic cold stress. Metabolic changes in the liver in mice housed at thermoneutral, standard and cold temperatures remain unknown. In the present study, we isolated lipid droplets and mitochondria from their livers in a comparative proteomic study aiming to investigate the changes. According to proteomic analysis, mitochondrial tricarboxylic acid cycle (TCA cycle) and retinol metabolism are enhanced, whereas oxidative phosphorylation is not affected obviously under cold conditions, suggesting that liver mitochondria may increase TCA cycle capacity in biosynthetic pathways, as well as retinol metabolism, to help the liver to adapt. Based on proteomic and immunoblotting results, perilipin 5 and major urinary proteins are increased significantly, whereas mitochondrial pyruvate carrier is decreased dramatically under cold conditions, indicating their involvement in liver adaptation.

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Section: Discussionsupporting

confidence: 92%

Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures

Zhou

Liu

et al. 2019

FEBS Letters

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“…44 In our previous study, we have shown that GPX1 is down‐regulated in liver mitochondria of apoE −/− mice, compared to C57BL/6J mice. 45 Furthermore, it was demonstrated that deficiency of GPX1 accelerates the progression of atherosclerosis in apoE −/− mice. 46–47 Of note, G6PDX, up‐regulated by Alda‐1 at the gene level, catalyzes the rate‐determining step in the pentose phosphate pathway and produces the NADPH necessary to fuel glutathione recycling for use by GPX to remove excess hydrogen peroxides.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Aldehyde Dehydrogenase Activation by Alda‐1 Inhibits Atherosclerosis and Attenuates Hepatic Steatosis in Apolipoprotein E‐Knockout Mice

Stachowicz

Olszanecki

Suski

et al. 2014

JAHA

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BackgroundMitochondrial dysfunction has been shown to play an important role in the development of atherosclerosis and nonalcoholic fatty liver disease (NAFLD). Mitochondrial aldehyde dehydrogenase (ALDH2), an enzyme responsible for the detoxification of reactive aldehydes, is considered to exert protective function in mitochondria. We investigated the influence of Alda‐1, an activator of ALDH2, on atherogenesis and on the liver steatosis in apolipoprotein E knockout (apoE−/−) mice.Methods and ResultsAlda‐1 caused decrease of atherosclerotic lesions approximately 25% as estimated by “en face” and “cross‐section” methods without influence on plasma lipid profile, atherosclerosis‐related markers of inflammation, and macrophage and smooth muscle content in the plaques. Plaque nitrotyrosine was not changed upon Alda‐1 treatment, and there were no changes in aortic mRNA levels of factors involved in antioxidative defense, regulation of apoptosis, mitogenesis, and autophagy. Hematoxylin/eosin staining showed decrease of steatotic changes in liver of Alda‐1‐treated apoE−/− mice. Alda‐1 attenuated formation of 4‐hydroxy‐2‐nonenal (4‐HNE) protein adducts and decreased triglyceride content in liver tissue. Two‐dimensional electrophoresis coupled with mass spectrometry identified 20 differentially expressed mitochondrial proteins upon Alda‐1 treatment in liver of apoE−/− mice, mostly proteins related to metabolism and oxidative stress. The most up‐regulated were the proteins that participated in beta oxidation of fatty acids.ConclusionsCollectively, Alda‐1 inhibited atherosclerosis and attenuated NAFLD in apoE−/− mice. The pattern of changes suggests a beneficial effect of Alda‐1 in NAFLD; however, the exact liver functional consequences of the revealed alterations as well as the mechanism(s) of antiatherosclerotic Alda‐1 action require further investigation.

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“…Pooling also reduced the experimental variations in the data and minimized the data files subjected to computer‐intensive comparative analysis. Many published research findings were from pooled samples [8,11]. Our pretest data indicated that the profiles for the pooled samples could generally mirror the protein profiles of individuals.…”

Section: Resultsmentioning

confidence: 68%

“…Gel electrophoresis and image analysis were performed as described elsewhere [8] with some modifications. Each sample, containing 150 μg protein with the final volume of 410 μL solubilization buffer, was loaded onto 24‐cm immobiline DryStrips (pH 3–10, non‐linear) on an IEF system (Bio‐Rad).…”

Section: Methodsmentioning

confidence: 99%

Proteomic analysis of sputum in patients with active pulmonary tuberculosis

Guan

et al. 2012

Clinical Microbiology and Infection

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The protein composition of sputum most faithfully reflects the state of the lungs. The aim of this study was to determine whether relative qualitative and quantitative differences in protein expression of sputum could be related to active pulmonary tuberculosis. Sputum samples were collected from 65 patients with active pulmonary tuberculosis and 38 healthy controls. Comprehensive proteomic approaches were used to profile the proteome changes of host sputum in response to Mycobacterium tuberculosis infection using two-dimensional electrophoresis in combination with matrix-assisted laser desorption ionization time-of-flight/time-of-flight mass spectrometry. Mascot software was used to identify proteins from protein databases. Enzyme-linked immunosorbent assay was used to confirm the proteomic results. A total of 62 differentially expressed proteins were identified, among which, 15 proteins were up-regulated and 47 proteins were down-regulated in the tuberculosis sputum compared with the controls. Bacterial protein UqhC was the most increased protein, whereas serum albumin was the most decreased protein in the tuberculosis sputum compared with the controls. The enzyme-linked immunosorbent assay analysis was consistent with proteomic data. Bioinformatics analysis suggested that multiple host cell pathways were involved in the tuberculosis infection processes, including acute phase response, signal transduction, cytoskeleton structure, immune response and so on. In all, for the first time, our results revealed that a number of proteins were differentially expressed during active pulmonary tuberculosis infection. These data will provide valuable clues for further investigation of tuberculosis pathogenesis and biomarkers for detection of active pulmonary tuberculosis infection.

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Proteomic analysis of changes in protein expression in liver mitochondria in apoE knockout mice

Cited by 18 publications

References 30 publications

Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures

Comparative proteomic study of liver lipid droplets and mitochondria in mice housed at different temperatures

Mitochondrial Aldehyde Dehydrogenase Activation by Alda‐1 Inhibits Atherosclerosis and Attenuates Hepatic Steatosis in Apolipoprotein E‐Knockout Mice

Proteomic analysis of sputum in patients with active pulmonary tuberculosis

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