Proteomic Analysis of Cassava Mosaic Virus (CMV) Responsive Proteins in Cassava Leaf

Abstract

Proteomics is becoming an increasingly important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying hostpathogen interaction, plant physiology, development and differentiation. Cassava mosaic disease (CMD), caused by cassava mosaic virus (CMV), is the most serious disease in cassava. However, the molecular mechanisms underlying CMD in cassava during CMV infection is not yet clearly understood. The current study determined and identifies the differentially expressed proteins from cassava leaves during the infection of CMV viz., Indian Cassava mosaic virus (ICMV) and Sri Lankan Cassava Mosaic Virus (SLCMV). 2D gel electrophoresis was used to identify the cassava responsive proteins during the virus infection and the differentially expressed proteins were analysed by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. There are 19 proteins were differentially expressed in cassava leaves by CMV infection. Among them 18 were giving good spectra by MALDI-TOF mass spectrometry. Analysis of Peptide Mass Fingerprint (PMF) data of these 18 proteins revealed the identity of the differentially expressed proteins, which suggest their importance and relevance on plant growth and development, and defence. This work paves the way towards a comprehensive analysis of CMV infection of cassava. Identification of the differentially expressed proteins by their sequence homology to known proteins suggests a possible direct or indirect role on plant defence during CMV infection. This study revealed the differentially expressed proteins, expressed during interaction between cassava and CMV that might play important roles either in viral pathogenesis or resistance.

Proteome Mapping of South African Cassava Mosaic Virus-Infected Susceptible and Tolerant Landraces of Cassava

“…Ribosomal proteins are not only known to be components of the protein synthesis machinery, but are also involved in stress signalling [ 49 ]. In cassava infected with Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV), components of the 80S ribosome and 40S small subunit were upregulated in susceptible cassava cultivar H226 [ 32 ]. In a proteome study of the begomovirus, tomato chlorotic mottle virus (ToCMoV), in resistant tomato, two ribosomal proteins (40S ribosomal protein S7 and ribosomal protein L11/12) were also differentially expressed [ 25 ].…”

“…A cassava proteome has been investigated in storage roots and leaves under abiotic stress and physiological conditions [ 26 , 27 , 28 , 29 , 30 , 31 ]; however, there has only been one study that investigated the leaf proteome in response to viral infection. In this study, cassava was infected with both Indian cassava mosaic virus (ICMV) and Sri Lankan cassava mosaic virus (SLCMV), which causes CMD [ 32 ]. Proteins such as the DnaJ-like proteins involved in molecular chaperone functions, signalling, and disease resistance (ascorbate peroxidase and protein kinase-coding resistance proteins) were over-expressed, implying their involvement in viral infection resistance.…”