Proteomic analysis of BRCA1‐depleted cell line reveals a putative role for replication protein A2 up‐regulation in BRCA1 breast tumor development

Abstract

RPA2 up-regulation may thus be involved in the growth and/or survival of BRCA1 tumor cells and useful in immunohistochemical discrimination of triple-negative BRCA1 tumors.

New Insight on Entangled DNA Repair Pathways: Stable Silenced Human Cells for Unraveling the DDR Jigsaw

“…Among the targeted genes and in using our approach we have always obtained at least one vector able to impose long-term shut down greater than 80% as compared with control cells (as evidenced by realtime RT-PCR). Using this technology, more than 160 human genes in different human cell models such as HeLa (Ame et al, 2009, Amine et al, 2009, Aressy et al, 2008, Betous et al, 2009, Biard, 2007, Biard et al, 2005, Biard & Angulo, 2007, Boehler et al, 2011, Bouley et al, 2010, Britton et al, 2009a, Despras et al, 2007, Godon et al, 2008, Le May et al, 2010b, Ousset et al, 2010, Pennarun et al, 2008, Pennarun et al, 2010, Wu et al, 2007, U2OS (Betous et al, 2009) and MRC5-V1 (Bouquet et al, 2011, Britton et al, 2009b, Schmutz et al, 2010 cells have been silenced. Our approach has also been successfully tested in other human tumorderived cell lines, such as RKO (Biard & Angulo, 2007), HCT-116 (Aressy et al, 2008), Caco2 (Coant et al, 2010), SH-SY5Y cells (Schulte et al, 2008), MCF7, MDA-MB 231, K562, UT7 (papers in preparation), and even in mouse NIH-3T3 cells (Meulle et al, 2008).…”