Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ)

Abstract

Herein, we performed a proteomic analysis of tenderloin and flank steaks from Simmental cattle using the isobaric tags for a relative and absolute quantification (iTRAQ) approach. We identified 17 amino acids in both steaks, and Gly, Cys, Ile, Lys, and Pro differed most in abundance between the steak types (p < 0.05). A comparison of the expression patterns in steaks revealed 128 differentially expressed proteins (DEPs), of which 44 were up-regulated and 84 were down-regulated. Furthermore, 27 DEPs (p < 0.05) were subjected to gene ontology (GO) analysis, and many were found to be related to oxidation-reduction, metabolism, hydrogen ion transmembrane transport, transport, the tricarboxylic acid (TCA) cycle, mitochondrial electron transport, and the conversion of nicotinamide adenine dinucleotide (NADH) to ubiquinone. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis also implicated these DEPs in various signalling pathways, including oxidative phosphorylation, cardiac muscle contraction, the TCA cycle, biosynthesis, and the metabolism. These findings provide a new insight into key proteins involved in the determination of amino acid composition in beef.

Strand-Specific RNA Sequencing Reveals Gene Expression Patterns in F1 Chick Breast Muscle and Liver after Hatching

“…Oxidative phosphorylation is a pathway that uses energy released from the oxidation of nutrients to produce ATP [61]. A recent study showed the downregulation of differentially expressed proteins associated with oxidative phosphorylation, indicating a high level of energy metabolism in muscle [62]. The TGF-beta signaling pathway is involved in cell proliferation, differentiation, and signal transduction [63].…”

Proteomic Analysis and Comparison of Protein in Tibetan pork and Ordinary pork using TMT-coupled LC-MS/MS

“…The e cient utilization of energy in Tibetan pigs was also helpful for subsequent research on the meat quality, and speculating the changes in energy reserves and metabolism in human body after eating Tibetan pork. Furthermore, proteomics studies of other meats have found that differentially expressed proteins from grouper llets were mainly involved in carbon metabolism and microbial metabolism in diverse environments [44], most of the differentially expressed proteins of beef tenderloin and ank steaks were related to amino acid and energy metabolism [45], and the differentially expressed proteins of chicken skeletal muscle were mainly involved in ribosome and muscle contraction pathways [46]. Tibetan pork's proteomics research results are different from other types of meats, its advantages are worthy of further study.…”

Insights from proteome to phosphorylated proteome: deciphering different regulatory mechanisms in goat muscles with high‐ and low‐meat quality

“…Recently, proteomics analysis based on the ‘isobaric tag’ for relative and absolute quantitation (iTRAQ) technology has been widely applied to the research of meat quality (Lei et al ., 2020). The iTRAQ technology exhibits high efficiency, which can enormously reduce the sample processing and experimental errors caused by different groups.…”

“…The iTRAQ technology exhibits high efficiency, which can enormously reduce the sample processing and experimental errors caused by different groups. It can systematically study as many proteins as possible in the organization, and detect protein present at both low and high levels (Shi et al ., 2018; Lei et al ., 2020). Phosphorylation proteomics is based on the enrichment and purification of phosphorylated peptides, with the difference of phosphorylated proteins analyzed by mass spectrometry (MS).…”