Proteomic analysis of Arabidopsis root gravitropism

Kamada, Motoshi; Higashitani, Akihiro; Ishioka, Noriaki

doi:10.2187/bss.19.148

Cited by 26 publications

(13 citation statements)

References 31 publications

(26 reference statements)

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“…Because it is well documented that the regulation of cellular events can be determined by proteins with no apparent change in mRNA steady‐state levels, proteomic analysis should provide important information. The first proteomic analysis on gravitropism was conducted on Arabidopsis root (Kamada et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation

Azri

Chambon

Herbette

et al. 2009

Physiologia Plantarum

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Gravity is a constant force guiding the direction of plant growth. In young poplar stem, reorientation of the apical region is mainly obtained by differential growth of elongating primary tissues. At the base, where elongation is achieved but where the cambium is active, reorientation is due to asymmetrical formation of reaction wood. After 45 min of gravistimulation, the stem showed no reorientation, but 1 week later, reaction wood was observed at the base of the stem. To determine the molecular mechanisms taking place at the top and base of the stem, after 45 min or 1 week of inclination, the changes induced in protein accumulation were studied by two-dimensional polyacrylamide gel electrophoresis and quantitatively analyzed using image analysis software. Around 300 protein spots were reproducibly detected and analyzed. Forty percent of these proteins showed significant changes after inclination. Mass spectrometry analysis of 135 spots led to the identification of 60 proteins involved in a wide range of activities and metabolisms. Very different patterns of protein expression were obtained according to conditions tested, highlighting the complexity of gravitropic responses. Our results suggest that primary and secondary tissues present specific mechanisms to sense reorientation and to respond to inclination. Some selected proteins are discussed.

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Section: Introductionmentioning

confidence: 99%

Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation

Azri

Chambon

Herbette

et al. 2009

Physiologia Plantarum

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“…In addition, we observed alterations in levels of transcripts involved in signaling, DNA regulation and various cellular processes at 0.5 h and 6 h. Plant glutathione S-transferases (GSTs) were suggested to be related to plant gravitropism [24], [47]. Moseyko (2005) found that GST belongs to the oxidative burst plant defense group, which is the largest functional category of gravity-regulated transcripts in Arabidopsis , but the gravitropic functions of those corresponding transcripts in rice are not clear.…”

Section: Resultsmentioning

confidence: 98%

Microarray Analyses and Comparisons of Upper or Lower Flanks of Rice Shoot Base Preceding Gravitropic Bending

Mei

Zang

et al. 2013

PLoS ONE

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Gravitropism is a complex process involving a series of physiological pathways. Despite ongoing research, gravitropism sensing and response mechanisms are not well understood. To identify the key transcripts and corresponding pathways in gravitropism, a whole-genome microarray approach was used to analyze transcript abundance in the shoot base of rice (Oryza sativa sp. japonica) at 0.5 h and 6 h after gravistimulation by horizontal reorientation. Between upper and lower flanks of the shoot base, 167 transcripts at 0.5 h and 1202 transcripts at 6 h were discovered to be significantly different in abundance by 2-fold. Among these transcripts, 48 were found to be changed both at 0.5 h and 6 h, while 119 transcripts were only changed at 0.5 h and 1154 transcripts were changed at 6 h in association with gravitropism. MapMan and PageMan analyses were used to identify transcripts significantly changed in abundance. The asymmetric regulation of transcripts related to phytohormones, signaling, RNA transcription, metabolism and cell wall-related categories between upper and lower flanks were demonstrated. Potential roles of the identified transcripts in gravitropism are discussed. Our results suggest that the induction of asymmetrical transcription, likely as a consequence of gravitropic reorientation, precedes gravitropic bending in the rice shoot base.

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“…Dissolved protein (1µl) plus matrix i.e. CHCA/ SA (1µl) was spotted on to MALDI plate and scanned 42,43 . The mass/charge region 1000 to 10000 was selected to obtain protein fingerprint pattern for all selected plants.…”

Section: Fig 10: Shows Maldi-tof Spectra Of Hemidesmus Indicus Freshmentioning

confidence: 99%

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2010

IJPSR

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Randomly amplified polymorphisms DNA (RAPD) has been widely used for authentication of plant species of medicinal importance. It is particularly useful in case of plants that are frequently substituted or adulterated with other species or varieties that are morphologically and/or phytochemically indistinguishable. In this study Hemidesmus indicus and its substitute plants were selected to develop gene and protein based coding for medicinal plants. DNA and protein was isolated from all the plants, i.e. the medicinal plant as well as the substitute plants. A modified DNA and protein extraction procedure was used for dry roots and powder of Hemidesmus indicus. Further a RAPD technique was used to develop DNA fingerprint pattern for all plants using self designed random primers. Sequence Characteristic Amplified Region (SCAR) marker was developed for Hemidesmus indicus. MALDI-TOF technique was used to differentiate between plant parts. Unique markers were developed for Hemidesmus indicus, to identify whether main plant is used or substitute plant, which part is added in formulation.

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Proteomic analysis of Arabidopsis root gravitropism

Cited by 26 publications

References 31 publications

Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation

Proteome analysis of apical and basal regions of poplar stems under gravitropic stimulation

Microarray Analyses and Comparisons of Upper or Lower Flanks of Rice Shoot Base Preceding Gravitropic Bending

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