Proteomic analysis of AQP11-null kidney: Proximal tubular type polycystic kidney disease

Saito, Tatsuya; Tanaka, Yasuko; Morishita, Yoshiyuki; Ishibashi, Keiichiro

doi:10.1016/j.bbrep.2017.11.003

Cited by 16 publications

(15 citation statements)

References 41 publications

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“…In addition, PSP/REG Iα has been demonstrated to increase β cell growth and regeneration by inducing cellular proliferation. PSP/REG Iα messenger ribonucleic acid (mRNA) is mainly found in the pancreas, but its expression has also been detected in the gastric mucosa and the kidneys [9,12]. It has been found in the urine and renal calculi of healthy individuals [13], which suggested a physiological role of PSP/REG Iα in the kidney.…”

Section: Introductionmentioning

confidence: 99%

Association of Serum PSP/REG Iαwith Renal Function in Type 2 Diabetes Mellitus

HuiMin

Zhu

Lin

et al. 2020

Journal of Diabetes Research

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Purpose. Pancreatic stone protein/regenerating protein I (PSP/REG Iα) is a secretory protein mainly detected in the pancreas. Recent studies revealed increased serum PSP/REG Iα levels may reflect renal dysfunction. The purpose of this study was to detect the relationship between PSP/REG Iα and renal function in subjects with and without type 2 diabetes mellitus (T2DM). Methods. This cross-sectional study was conducted at Zhongda Hospital, affiliated with Southeast University in China. Serum PSP/REG Iα levels were measured using a method of enzyme-linked immunosorbent assay. Baseline characteristics and biochemical parameters, such as blood urea nitrogen (BUN), serum creatinine (SCr), and uric acid (UA), were collected. The estimated glomerular filtration rate (eGFR) of each individual was calculated using the diagnostic criteria for renal function. Correlations between PSP/REG Iα and renal function parameters were analyzed by Spearman's rank correlation coefficient using SPSS 20.0 software. Results. Serum PSP/REG Iα levels were significantly higher in T2DM patients than those without T2DM (P < 0:05). The level of PSP/REG Iα was positively correlated with age, SCr, and BUN and negatively correlated with eGFR. The ordinal multiple logistic regression analysis further illustrated that PSP/REG Iα levels were negatively related with eGFR in both groups after adjusting for other parameters. Conclusions. Serum PSP/REG Iα level is significantly upregulated in T2DM patients and reflects renal function in both T2DM and nondiabetic control groups. The relationship between PSP/REG Iα and eGFR suggested that PSP/REG Iα might be a potential indicator of renal dysfunction.

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Section: Introductionmentioning

confidence: 99%

Association of Serum PSP/REG Iαwith Renal Function in Type 2 Diabetes Mellitus

HuiMin

Zhu

Lin

et al. 2020

Journal of Diabetes Research

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“…Studies of renal mRNAs have suggested that cell proliferation, remodeling of the extracellular matrix, apoptosis, and ER stress are possibly involved in the pathogenesis of the kidney injury [12] . Analytical studies of proteins in Aqp11 -/- mice have shown that decreased levels of mitochondrial proteins are likely associated with kidney injury due to AQP11 deficiency [13] . Previously, our group has demonstrated that in Aqp11 sjds/sjds mice the renal level of superoxide was increased and that an antioxidant ameliorated the reactive oxygen species (ROS)-related kidney injury in Aqp11 sjds /+ mice [14] .…”

Section: Introductionmentioning

confidence: 99%

Involvement of the NADPH oxidase 2 pathway in renal oxidative stress in Aqp11-/- mice

Hoshino

Sonoda

Nishimura

et al. 2019

Biochemistry and Biophysics Reports

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Aquaporin-11 (AQP11) is an intracellular AQP. Several studies with Aqp11-/- mice have shown that AQP11 has a role in normal development of the kidney after birth. Our previous studies have suggested that alteration of oxygen homeostasis may be involved in the kidney injury caused by AQP11 deficiency, although the underlying mechanism is largely unknown. To clarify this issue, we examined genes that are related to oxygen homeostasis in Aqp11-/- mice. Among 62 genes that are involved in oxygen homeostasis, 35 were upregulated by more than 2-fold in Aqp11-/- mice in comparison with wild-type mice. Pathway analysis using these genes extracted the pathway responsible for production of reactive oxygen species in macrophages. As expression of the genes involved in the NADPH oxidase 2 (NOX2) complex was dramatically increased by more than 14-fold, we further analyzed NOX2 at the protein level. Immunoblotting analysis demonstrated a dramatic increase of NOX2 protein in the kidney of Aqp11-/- mice, and immunohistochemistry showed that NOX2 protein and a marker protein for macrophages were increased in the renal interstitium. These results indicate that NOX2-induced oxidative stress accompanied by macrophage infiltration plays an important role in alteration of oxygen homeostasis in Aqp11-/- mice.

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“…Capillary electrophoresis-mass spectrometry (CE-MS) Kaiser et al (2003), Wittke et al (2003), Metzger et al (2010), Alkhalaf et al (2010), Rossing et al (2008), Good et al (2010), Mischak, Delles, Klein, and Schanstra (2010) Tandem mass tag (TMT) Saito, Tanaka, Morishita, and Ishibashi (2017) single-cell genomics, transcriptomes, and protein abundance from comparable individual cells will provide substantial additional insight into the regulation of homeostatic and pathologic cell phenotype and function (Macaulay, Ponting, & Voet, 2017). Zhou et al (2019) demonstrated that integrating single cell RNA-seq, single cell DNA methylation analysis, and single cell combined displacement preamplification and PCR amplification (MALBAC) wholegenome sequencing revealed the molecular dynamics in an in vitro model of human implantation (Zhou et al, 2019).…”

Section: Proteomics Referencesmentioning

confidence: 99%